Squamous intraepithelial lesions of the anal squamocolumnar junction: Histopathological classification and HPV genotyping
Background: Human papillomavirus (HPV)-related anal cancer lesions are often found adjacent to the squamocolumnar junction (SCJ). We have assessed the histopathology and associated HPV genotypes in anal SCJ lesions in surgically excised anal warts in HIV-negative and âpositive patients. Methods: His...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-06-01
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| Series: | Papillomavirus Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405852116300374 |
| _version_ | 1818494923528208384 |
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| author | Omar Clavero Jenny McCloskey Vicente Marco Molina Beatriz Quirós Ignacio G. Bravo Silvia de Sanjosé F. Xavier Bosch Ville N. Pimenoff |
| author_facet | Omar Clavero Jenny McCloskey Vicente Marco Molina Beatriz Quirós Ignacio G. Bravo Silvia de Sanjosé F. Xavier Bosch Ville N. Pimenoff |
| author_sort | Omar Clavero |
| collection | DOAJ |
| description | Background: Human papillomavirus (HPV)-related anal cancer lesions are often found adjacent to the squamocolumnar junction (SCJ). We have assessed the histopathology and associated HPV genotypes in anal SCJ lesions in surgically excised anal warts in HIV-negative and âpositive patients. Methods: Histopathology identified 47 squamous intraepithelial lesions (SILs) adjacent to the SCJ amongst a total of 145 cases of clinically diagnosed anal condylomata. The anal SCJ lesions were further analyzed with p16, CK7 and p63 immunohistochemistry and HPV genotyping. Results: Sixteen (16/47) of the excised anal wart lesions contained HSIL; Three were HSIL and exclusively associated with oncogenic HPVs. A further thirteen (13/47) were mixed lesions. Of these eight were HSILs with LSIL and six were HSILs with papillary immature metaplasia (PIM); Ten of the mixed lesions were associated with one or more oncogenic HPVs, while three cases were exclusively associated with HPV6. Conclusions: Clinically diagnosed anal warts cannot be assumed to be limited to low-grade lesions as anal warts of the SCJ often show heterogeneous lesions, with coexistence of LSIL, PIM, and HSIL. Lesions showing PIM, however, may mimic HSIL, because they are hypercellular, but lack the nuclear atypia and conspicuous mitotic activity of HSIL; and are p16 negative. Keywords: Anal squamocolumnar junction, Low-grade squamous intraepithelial lesion (LSIL), High-grade squamous intraepithelial lesion (HSIL), Papillary immature metaplasia (PIM), HPV, HIV |
| first_indexed | 2024-12-10T18:13:37Z |
| format | Article |
| id | doaj.art-e2983701e06f4c9cb6d0c55cf616114e |
| institution | Directory Open Access Journal |
| issn | 2405-8521 |
| language | English |
| last_indexed | 2024-12-10T18:13:37Z |
| publishDate | 2017-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Papillomavirus Research |
| spelling | doaj.art-e2983701e06f4c9cb6d0c55cf616114e2022-12-22T01:38:24ZengElsevierPapillomavirus Research2405-85212017-06-0131117Squamous intraepithelial lesions of the anal squamocolumnar junction: Histopathological classification and HPV genotypingOmar Clavero0Jenny McCloskey1Vicente Marco Molina2Beatriz Quirós3Ignacio G. Bravo4Silvia de Sanjosé5F. Xavier Bosch6Ville N. Pimenoff7Infections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology, Barcelona, Spain; Hospital Quiron Salud, Barcelona, SpainSexual Health Clinic, Royal Perth Hospital, School of Laboratory and Pathology Medicine, University of WA, Perth, AustraliaHospital Quiron Salud, Barcelona, SpainInfections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology, Barcelona, SpainInfections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology, Barcelona, Spain; Bellvitge Institute of Biomedical Research (IDIBELL), Barcelona, SpainInfections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology, Barcelona, Spain; CIBER en EpidemiologÃa y Salud Pública (CIBERESP), SpainInfections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology, Barcelona, SpainInfections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology, Barcelona, Spain; Bellvitge Institute of Biomedical Research (IDIBELL), Barcelona, Spain; Correspondence to: Infections and Cancer Laboratory, Bellvitge Institute for Biomedical Research (IDIBELL), Catalan Institute of Oncology, Avda. Gran VÃa, KM 199-203, 08908 L'Hospitalet de Llobregat, Barcelona, Spain.Background: Human papillomavirus (HPV)-related anal cancer lesions are often found adjacent to the squamocolumnar junction (SCJ). We have assessed the histopathology and associated HPV genotypes in anal SCJ lesions in surgically excised anal warts in HIV-negative and âpositive patients. Methods: Histopathology identified 47 squamous intraepithelial lesions (SILs) adjacent to the SCJ amongst a total of 145 cases of clinically diagnosed anal condylomata. The anal SCJ lesions were further analyzed with p16, CK7 and p63 immunohistochemistry and HPV genotyping. Results: Sixteen (16/47) of the excised anal wart lesions contained HSIL; Three were HSIL and exclusively associated with oncogenic HPVs. A further thirteen (13/47) were mixed lesions. Of these eight were HSILs with LSIL and six were HSILs with papillary immature metaplasia (PIM); Ten of the mixed lesions were associated with one or more oncogenic HPVs, while three cases were exclusively associated with HPV6. Conclusions: Clinically diagnosed anal warts cannot be assumed to be limited to low-grade lesions as anal warts of the SCJ often show heterogeneous lesions, with coexistence of LSIL, PIM, and HSIL. Lesions showing PIM, however, may mimic HSIL, because they are hypercellular, but lack the nuclear atypia and conspicuous mitotic activity of HSIL; and are p16 negative. Keywords: Anal squamocolumnar junction, Low-grade squamous intraepithelial lesion (LSIL), High-grade squamous intraepithelial lesion (HSIL), Papillary immature metaplasia (PIM), HPV, HIVhttp://www.sciencedirect.com/science/article/pii/S2405852116300374 |
| spellingShingle | Omar Clavero Jenny McCloskey Vicente Marco Molina Beatriz Quirós Ignacio G. Bravo Silvia de Sanjosé F. Xavier Bosch Ville N. Pimenoff Squamous intraepithelial lesions of the anal squamocolumnar junction: Histopathological classification and HPV genotyping Papillomavirus Research |
| title | Squamous intraepithelial lesions of the anal squamocolumnar junction: Histopathological classification and HPV genotyping |
| title_full | Squamous intraepithelial lesions of the anal squamocolumnar junction: Histopathological classification and HPV genotyping |
| title_fullStr | Squamous intraepithelial lesions of the anal squamocolumnar junction: Histopathological classification and HPV genotyping |
| title_full_unstemmed | Squamous intraepithelial lesions of the anal squamocolumnar junction: Histopathological classification and HPV genotyping |
| title_short | Squamous intraepithelial lesions of the anal squamocolumnar junction: Histopathological classification and HPV genotyping |
| title_sort | squamous intraepithelial lesions of the anal squamocolumnar junction histopathological classification and hpv genotyping |
| url | http://www.sciencedirect.com/science/article/pii/S2405852116300374 |
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