Over-expression of mammaglobin-B in canine mammary tumors
Abstract Background Mammaglobin, a member of secretoglobin family has been recognized as a breast cancer associated protein. Though the exact function of the protein is not fully known, its expression has been reported to be upregulated in human breast cancer.We focused on studying the expression of...
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1507-z |
| _version_ | 1818864460981338112 |
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| author | Mamta Pandey B. V. Sunil Kumar Kuldip Gupta Ram Saran Sethi Ashwani Kumar Ramneek Verma |
| author_facet | Mamta Pandey B. V. Sunil Kumar Kuldip Gupta Ram Saran Sethi Ashwani Kumar Ramneek Verma |
| author_sort | Mamta Pandey |
| collection | DOAJ |
| description | Abstract Background Mammaglobin, a member of secretoglobin family has been recognized as a breast cancer associated protein. Though the exact function of the protein is not fully known, its expression has been reported to be upregulated in human breast cancer.We focused on studying the expression of mammaglobin-B gene and protein in canine mammary tumor (CMT) tissue. Expression of mammaglobin-B mRNA and protein were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC), respectively. Results High levels of mammaglobin-B mRNA expression (6.663 ± 0.841times) was observed in CMT as compared to age and breed matched healthy controls. Further, expression of mammaglobin-B protein was detected in paraffin-embedded mammary tumor tissues from the same subjects by IHC. Mammaglobin-B protein was overexpressed only in 6.67% of healthy mammary glands while, a high level of its expression was scored in 76.7% of the CMT subjects. Moreover, no significant differences in terms of IHC score and qRT-PCR score with respect to CMT histotypes or tumor grades were observed, indicating that mammaglobin-B over-expression occurred irrespective of CMT types or grades. Conclusion Overall, significantly increased expression of mammaglobin-B protein was found in CMTs with respect to healthy mammary glands, which positively correlates to its transcript. These findings suggest that overexpression of mammaglobin-B is associated with tumors of canine mammary glands. |
| first_indexed | 2024-12-19T10:32:01Z |
| format | Article |
| id | doaj.art-e29b1f553802430386c3bb882fc10de5 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-19T10:32:01Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-e29b1f553802430386c3bb882fc10de52022-12-21T20:25:44ZengBMCBMC Veterinary Research1746-61482018-06-011411810.1186/s12917-018-1507-zOver-expression of mammaglobin-B in canine mammary tumorsMamta Pandey0B. V. Sunil Kumar1Kuldip Gupta2Ram Saran Sethi3Ashwani Kumar4Ramneek Verma5School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences UniversitySchool of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences UniversityDepartment of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences UniversitySchool of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences UniversityDepartment of Veterinary Surgery and Radiology, Guru Angad Dev Veterinary and Animal Sciences UniversitySchool of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences UniversityAbstract Background Mammaglobin, a member of secretoglobin family has been recognized as a breast cancer associated protein. Though the exact function of the protein is not fully known, its expression has been reported to be upregulated in human breast cancer.We focused on studying the expression of mammaglobin-B gene and protein in canine mammary tumor (CMT) tissue. Expression of mammaglobin-B mRNA and protein were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC), respectively. Results High levels of mammaglobin-B mRNA expression (6.663 ± 0.841times) was observed in CMT as compared to age and breed matched healthy controls. Further, expression of mammaglobin-B protein was detected in paraffin-embedded mammary tumor tissues from the same subjects by IHC. Mammaglobin-B protein was overexpressed only in 6.67% of healthy mammary glands while, a high level of its expression was scored in 76.7% of the CMT subjects. Moreover, no significant differences in terms of IHC score and qRT-PCR score with respect to CMT histotypes or tumor grades were observed, indicating that mammaglobin-B over-expression occurred irrespective of CMT types or grades. Conclusion Overall, significantly increased expression of mammaglobin-B protein was found in CMTs with respect to healthy mammary glands, which positively correlates to its transcript. These findings suggest that overexpression of mammaglobin-B is associated with tumors of canine mammary glands.http://link.springer.com/article/10.1186/s12917-018-1507-zCanineExpressionImmunohistochemistryMammaglobin-BMammary tumorqRT-PCR |
| spellingShingle | Mamta Pandey B. V. Sunil Kumar Kuldip Gupta Ram Saran Sethi Ashwani Kumar Ramneek Verma Over-expression of mammaglobin-B in canine mammary tumors BMC Veterinary Research Canine Expression Immunohistochemistry Mammaglobin-B Mammary tumor qRT-PCR |
| title | Over-expression of mammaglobin-B in canine mammary tumors |
| title_full | Over-expression of mammaglobin-B in canine mammary tumors |
| title_fullStr | Over-expression of mammaglobin-B in canine mammary tumors |
| title_full_unstemmed | Over-expression of mammaglobin-B in canine mammary tumors |
| title_short | Over-expression of mammaglobin-B in canine mammary tumors |
| title_sort | over expression of mammaglobin b in canine mammary tumors |
| topic | Canine Expression Immunohistochemistry Mammaglobin-B Mammary tumor qRT-PCR |
| url | http://link.springer.com/article/10.1186/s12917-018-1507-z |
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