Effects of Long-Term Low-Protein Diets Supplemented with Sodium Dichloroacetate and Glucose on Metabolic Biomarkers and Intestinal Microbiota of Finishing Pigs
The objective of this study was to evaluate the effects of low-protein (LP) diets supplemented with sodium dichloroacetate (DCA) and glucose (GLUC) on metabolic markers and intestinal microbiota of finishing pigs. A total of 80 crossbred growing barrows were allocated randomly to one of the five tre...
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MDPI AG
2022-09-01
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| Online Access: | https://www.mdpi.com/2076-2615/12/19/2522 |
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| author | Yetong Xu Huiyuan Chen Ke Wan Zhiru Tang Weizhong Sun Liuting Wu Zhongxiang Ren Qi Ding Kaiyang Liang Zhihong Sun |
| author_facet | Yetong Xu Huiyuan Chen Ke Wan Zhiru Tang Weizhong Sun Liuting Wu Zhongxiang Ren Qi Ding Kaiyang Liang Zhihong Sun |
| author_sort | Yetong Xu |
| collection | DOAJ |
| description | The objective of this study was to evaluate the effects of low-protein (LP) diets supplemented with sodium dichloroacetate (DCA) and glucose (GLUC) on metabolic markers and intestinal microbiota of finishing pigs. A total of 80 crossbred growing barrows were allocated randomly to one of the five treatments, including the normal protein level diet (CON), the LP diets, LP with 120 mg/kg DCA (LP + DCA) or 1.8% glucose (LP + GLUC), and LP with 120 mg/kg DCA and 1.8% glucose (LP + DCA + GLUC). The LP diet increased the plasma HDL, triglyceride, and cholesterol concentrations and reduced the bile acid, urea nitrogen, albumin, and total protein concentrations compared to the CON diet (<i>p</i> < 0.05). The LP + DCA + GLUC diet reduced the plasma VLDL, triglyceride, and cholesterol concentrations and increased the bile acid concentration compared with the LP diet (<i>p</i> < 0.05). Pigs fed the LP + DCA and LP + GLUC diets showed reduced 3-Hydroxy-3-Methylglutaryl-CoA Reductase content and increased Cytochrome P450 Family 7 Subfamily A Member 1 activity of liver compared that of the CON diet (<i>p</i> < 0.05). Moreover, the LP diets with or without DCA and GLUC supplementation increased the relative abundance of colonic microbiota related to carbohydrate fermentation in finishing pigs. In conclusion, 120 mg/kg DCA or 1.8% GLUC supplementation in an LP diet modulated the hepatic lipid metabolism of pigs, while the DCA along with GLUC supplementation likely improved the lipid metabolism by stimulating bile acid secretion. |
| first_indexed | 2024-03-09T22:09:52Z |
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| id | doaj.art-e29c6881bc5e4ea0ae917353bbcd48b0 |
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| language | English |
| last_indexed | 2024-03-09T22:09:52Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-e29c6881bc5e4ea0ae917353bbcd48b02023-11-23T19:35:02ZengMDPI AGAnimals2076-26152022-09-011219252210.3390/ani12192522Effects of Long-Term Low-Protein Diets Supplemented with Sodium Dichloroacetate and Glucose on Metabolic Biomarkers and Intestinal Microbiota of Finishing PigsYetong Xu0Huiyuan Chen1Ke Wan2Zhiru Tang3Weizhong Sun4Liuting Wu5Zhongxiang Ren6Qi Ding7Kaiyang Liang8Zhihong Sun9Laboratory for Bio-Feed and Molecular Nutrition, Department of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaLaboratory for Bio-Feed and Molecular Nutrition, Department of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaLaboratory for Bio-Feed and Molecular Nutrition, Department of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaLaboratory for Bio-Feed and Molecular Nutrition, Department of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaLaboratory for Bio-Feed and Molecular Nutrition, Department of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaLaboratory for Bio-Feed and Molecular Nutrition, Department of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaLaboratory for Bio-Feed and Molecular Nutrition, Department of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaLaboratory for Bio-Feed and Molecular Nutrition, Department of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaLaboratory for Bio-Feed and Molecular Nutrition, Department of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaLaboratory for Bio-Feed and Molecular Nutrition, Department of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaThe objective of this study was to evaluate the effects of low-protein (LP) diets supplemented with sodium dichloroacetate (DCA) and glucose (GLUC) on metabolic markers and intestinal microbiota of finishing pigs. A total of 80 crossbred growing barrows were allocated randomly to one of the five treatments, including the normal protein level diet (CON), the LP diets, LP with 120 mg/kg DCA (LP + DCA) or 1.8% glucose (LP + GLUC), and LP with 120 mg/kg DCA and 1.8% glucose (LP + DCA + GLUC). The LP diet increased the plasma HDL, triglyceride, and cholesterol concentrations and reduced the bile acid, urea nitrogen, albumin, and total protein concentrations compared to the CON diet (<i>p</i> < 0.05). The LP + DCA + GLUC diet reduced the plasma VLDL, triglyceride, and cholesterol concentrations and increased the bile acid concentration compared with the LP diet (<i>p</i> < 0.05). Pigs fed the LP + DCA and LP + GLUC diets showed reduced 3-Hydroxy-3-Methylglutaryl-CoA Reductase content and increased Cytochrome P450 Family 7 Subfamily A Member 1 activity of liver compared that of the CON diet (<i>p</i> < 0.05). Moreover, the LP diets with or without DCA and GLUC supplementation increased the relative abundance of colonic microbiota related to carbohydrate fermentation in finishing pigs. In conclusion, 120 mg/kg DCA or 1.8% GLUC supplementation in an LP diet modulated the hepatic lipid metabolism of pigs, while the DCA along with GLUC supplementation likely improved the lipid metabolism by stimulating bile acid secretion.https://www.mdpi.com/2076-2615/12/19/2522low-protein dietsodium dichloroacetatemetabolic markersintestinal microbiotapigs |
| spellingShingle | Yetong Xu Huiyuan Chen Ke Wan Zhiru Tang Weizhong Sun Liuting Wu Zhongxiang Ren Qi Ding Kaiyang Liang Zhihong Sun Effects of Long-Term Low-Protein Diets Supplemented with Sodium Dichloroacetate and Glucose on Metabolic Biomarkers and Intestinal Microbiota of Finishing Pigs Animals low-protein diet sodium dichloroacetate metabolic markers intestinal microbiota pigs |
| title | Effects of Long-Term Low-Protein Diets Supplemented with Sodium Dichloroacetate and Glucose on Metabolic Biomarkers and Intestinal Microbiota of Finishing Pigs |
| title_full | Effects of Long-Term Low-Protein Diets Supplemented with Sodium Dichloroacetate and Glucose on Metabolic Biomarkers and Intestinal Microbiota of Finishing Pigs |
| title_fullStr | Effects of Long-Term Low-Protein Diets Supplemented with Sodium Dichloroacetate and Glucose on Metabolic Biomarkers and Intestinal Microbiota of Finishing Pigs |
| title_full_unstemmed | Effects of Long-Term Low-Protein Diets Supplemented with Sodium Dichloroacetate and Glucose on Metabolic Biomarkers and Intestinal Microbiota of Finishing Pigs |
| title_short | Effects of Long-Term Low-Protein Diets Supplemented with Sodium Dichloroacetate and Glucose on Metabolic Biomarkers and Intestinal Microbiota of Finishing Pigs |
| title_sort | effects of long term low protein diets supplemented with sodium dichloroacetate and glucose on metabolic biomarkers and intestinal microbiota of finishing pigs |
| topic | low-protein diet sodium dichloroacetate metabolic markers intestinal microbiota pigs |
| url | https://www.mdpi.com/2076-2615/12/19/2522 |
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