Quantitative molecular analysis of sentinel lymph node may be predictive of axillary node status in breast cancer classified by molecular subtypes.
To determine the performance of intraoperative one-step nucleic acid amplification (OSNA) assay in detecting sentinel lymph node metastases compared to postoperative histology taking into account breast cancer molecular classification and to evaluate whether the level of cytokeratin 19 mRNA copy num...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3606361?pdf=render |
| _version_ | 1811213180464005120 |
|---|---|
| author | Simonetta Buglioni Franco Di Filippo Irene Terrenato Beatrice Casini Enzo Gallo Ferdinando Marandino Carlo L Maini Rossella Pasqualoni Claudio Botti Simona Di Filippo Edoardo Pescarmona Marcella Mottolese |
| author_facet | Simonetta Buglioni Franco Di Filippo Irene Terrenato Beatrice Casini Enzo Gallo Ferdinando Marandino Carlo L Maini Rossella Pasqualoni Claudio Botti Simona Di Filippo Edoardo Pescarmona Marcella Mottolese |
| author_sort | Simonetta Buglioni |
| collection | DOAJ |
| description | To determine the performance of intraoperative one-step nucleic acid amplification (OSNA) assay in detecting sentinel lymph node metastases compared to postoperative histology taking into account breast cancer molecular classification and to evaluate whether the level of cytokeratin 19 mRNA copy number may be useful in predicting the likelihood of a positive axillary lymph node dissection. OSNA assay was performed in a prospective series of 903 consecutive sentinel lymph nodes from 709 breast cancer patients using 2 alternate slices of each sentinel lymph node. The remaining 2 slices were investigated by histology. Cytokeratin 19 mRNA copy number, which distinguishes negative cases (<250 copies), micrometastases (+, ≥250≤5000 copies) and macrometastases (++, >5000 copies), was compared to axillary lymph node dissection status and to the biological tumor profile. Concordance between OSNA and histopathology was 95%, specificity 95% and sensitivity 93%. Multiple Corresponce Analysis and logistic regression evidenced that positive axillary lymph node dissection was significantly associated with a higher cytokeratin 19 mRNA copy number (>5000; p<0.0001), HER2 subtype (p = 0.007) and lymphovascular invasion (p<0.0001). Conversely, breast cancer patients with cytokeratin 19 mRNA copy number <2000 mostly presented a luminal subtype and a negative axillary lymph node dissection. We confirmed that OSNA assay can provide standardized and reproducible results and that it represents a fast and quantitative tool for intraoperative evaluation of sentinel lymph node. Omission of axillary lymph node dissection could be proposed in patients presenting a sentinel lymph node with a cytokeratin 19 mRNA copy number <2000 and a Luminal tumor phenotype. |
| first_indexed | 2024-04-12T05:41:32Z |
| format | Article |
| id | doaj.art-e2a23360aea94d29af2d5d29300be160 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T05:41:32Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e2a23360aea94d29af2d5d29300be1602022-12-22T03:45:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5882310.1371/journal.pone.0058823Quantitative molecular analysis of sentinel lymph node may be predictive of axillary node status in breast cancer classified by molecular subtypes.Simonetta BuglioniFranco Di FilippoIrene TerrenatoBeatrice CasiniEnzo GalloFerdinando MarandinoCarlo L MainiRossella PasqualoniClaudio BottiSimona Di FilippoEdoardo PescarmonaMarcella MottoleseTo determine the performance of intraoperative one-step nucleic acid amplification (OSNA) assay in detecting sentinel lymph node metastases compared to postoperative histology taking into account breast cancer molecular classification and to evaluate whether the level of cytokeratin 19 mRNA copy number may be useful in predicting the likelihood of a positive axillary lymph node dissection. OSNA assay was performed in a prospective series of 903 consecutive sentinel lymph nodes from 709 breast cancer patients using 2 alternate slices of each sentinel lymph node. The remaining 2 slices were investigated by histology. Cytokeratin 19 mRNA copy number, which distinguishes negative cases (<250 copies), micrometastases (+, ≥250≤5000 copies) and macrometastases (++, >5000 copies), was compared to axillary lymph node dissection status and to the biological tumor profile. Concordance between OSNA and histopathology was 95%, specificity 95% and sensitivity 93%. Multiple Corresponce Analysis and logistic regression evidenced that positive axillary lymph node dissection was significantly associated with a higher cytokeratin 19 mRNA copy number (>5000; p<0.0001), HER2 subtype (p = 0.007) and lymphovascular invasion (p<0.0001). Conversely, breast cancer patients with cytokeratin 19 mRNA copy number <2000 mostly presented a luminal subtype and a negative axillary lymph node dissection. We confirmed that OSNA assay can provide standardized and reproducible results and that it represents a fast and quantitative tool for intraoperative evaluation of sentinel lymph node. Omission of axillary lymph node dissection could be proposed in patients presenting a sentinel lymph node with a cytokeratin 19 mRNA copy number <2000 and a Luminal tumor phenotype.http://europepmc.org/articles/PMC3606361?pdf=render |
| spellingShingle | Simonetta Buglioni Franco Di Filippo Irene Terrenato Beatrice Casini Enzo Gallo Ferdinando Marandino Carlo L Maini Rossella Pasqualoni Claudio Botti Simona Di Filippo Edoardo Pescarmona Marcella Mottolese Quantitative molecular analysis of sentinel lymph node may be predictive of axillary node status in breast cancer classified by molecular subtypes. PLoS ONE |
| title | Quantitative molecular analysis of sentinel lymph node may be predictive of axillary node status in breast cancer classified by molecular subtypes. |
| title_full | Quantitative molecular analysis of sentinel lymph node may be predictive of axillary node status in breast cancer classified by molecular subtypes. |
| title_fullStr | Quantitative molecular analysis of sentinel lymph node may be predictive of axillary node status in breast cancer classified by molecular subtypes. |
| title_full_unstemmed | Quantitative molecular analysis of sentinel lymph node may be predictive of axillary node status in breast cancer classified by molecular subtypes. |
| title_short | Quantitative molecular analysis of sentinel lymph node may be predictive of axillary node status in breast cancer classified by molecular subtypes. |
| title_sort | quantitative molecular analysis of sentinel lymph node may be predictive of axillary node status in breast cancer classified by molecular subtypes |
| url | http://europepmc.org/articles/PMC3606361?pdf=render |
| work_keys_str_mv | AT simonettabuglioni quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT francodifilippo quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT ireneterrenato quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT beatricecasini quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT enzogallo quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT ferdinandomarandino quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT carlolmaini quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT rossellapasqualoni quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT claudiobotti quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT simonadifilippo quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT edoardopescarmona quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes AT marcellamottolese quantitativemolecularanalysisofsentinellymphnodemaybepredictiveofaxillarynodestatusinbreastcancerclassifiedbymolecularsubtypes |