pH Coupled Citric Acid Feeding Strategy to Promote Spore Production of Bacillus licheniformis
The effects of citric acid addition coupled with pH on cell growth, metabolism, and spore yield of B. licheniformis were studied in a 30 L bioreactor, and the gene expression differences related to energy metabolism, oxidative stress effect, and amino acid transport at the log-phase were examined by...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030261 |
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| author | Yue DING Junfeng HE Ruofei GONG Hang DAI Xiong CHEN Yanan HUANG Zhi WANG |
| author_facet | Yue DING Junfeng HE Ruofei GONG Hang DAI Xiong CHEN Yanan HUANG Zhi WANG |
| author_sort | Yue DING |
| collection | DOAJ |
| description | The effects of citric acid addition coupled with pH on cell growth, metabolism, and spore yield of B. licheniformis were studied in a 30 L bioreactor, and the gene expression differences related to energy metabolism, oxidative stress effect, and amino acid transport at the log-phase were examined by qPCR. Results showed that: Citric acid supplement coupled with pH7.5 during 19.5~28 h was conducted, reaching a final content of 78 μmol/L in the medium. The feeding strategy prolonged the periods of cell growth and spore formation, and the peak cells (36 h) reached 4.2×1010 CFU/mL that was 42.9% higher than the control (without citric acid supplement). Meanwhile, spore yield (36 h) reached 4.1×1010 CFU/mL, increased by 215.4% compared with the control (24 h, 1.3×1010 CFU/mL). The qPCR assays showed that the expression levels of icd, zwf and cydA was 2.6, 4.1 and 1.9 times higher than that of the control, respectively. Meanwhile, brnQ, vpr, yvbW, katA, yqjM, ahpF, sodA and abrB were decreased by 88%, 18%, 65%, 89%, 10%, 90%, 41% and 60% compared with the control, respectively. The data suggested that citric acid supplementation enhanced the efficiencies of TCA cycle, HMP pathway and electron transport, weakened the oxidative stress effects on cells, and improved the phosphorylation level of Spo0A, thus promoting the sporulation efficiency. The results would provide theoretical supports for the industrial production of Bacillus fermentation efficiently. |
| first_indexed | 2024-04-11T00:00:33Z |
| format | Article |
| id | doaj.art-e2ae9aa4484d4189abd3c21e935254b8 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T00:00:33Z |
| publishDate | 2023-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-e2ae9aa4484d4189abd3c21e935254b82023-01-10T06:06:20ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-01-0144215215810.13386/j.issn1002-0306.20220302612022030261-2pH Coupled Citric Acid Feeding Strategy to Promote Spore Production of Bacillus licheniformisYue DING0Junfeng HE1Ruofei GONG2Hang DAI3Xiong CHEN4Yanan HUANG5Zhi WANG6Key Laboratory of Fermentation Engineering (Ministry of Education), Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, ChinaHenan Province Nanjiecun Group Co., Ltd., Luohe 462600, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, ChinaThe effects of citric acid addition coupled with pH on cell growth, metabolism, and spore yield of B. licheniformis were studied in a 30 L bioreactor, and the gene expression differences related to energy metabolism, oxidative stress effect, and amino acid transport at the log-phase were examined by qPCR. Results showed that: Citric acid supplement coupled with pH7.5 during 19.5~28 h was conducted, reaching a final content of 78 μmol/L in the medium. The feeding strategy prolonged the periods of cell growth and spore formation, and the peak cells (36 h) reached 4.2×1010 CFU/mL that was 42.9% higher than the control (without citric acid supplement). Meanwhile, spore yield (36 h) reached 4.1×1010 CFU/mL, increased by 215.4% compared with the control (24 h, 1.3×1010 CFU/mL). The qPCR assays showed that the expression levels of icd, zwf and cydA was 2.6, 4.1 and 1.9 times higher than that of the control, respectively. Meanwhile, brnQ, vpr, yvbW, katA, yqjM, ahpF, sodA and abrB were decreased by 88%, 18%, 65%, 89%, 10%, 90%, 41% and 60% compared with the control, respectively. The data suggested that citric acid supplementation enhanced the efficiencies of TCA cycle, HMP pathway and electron transport, weakened the oxidative stress effects on cells, and improved the phosphorylation level of Spo0A, thus promoting the sporulation efficiency. The results would provide theoretical supports for the industrial production of Bacillus fermentation efficiently.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030261bacillus licheniformiscitric acidbiomasssporesqpcr |
| spellingShingle | Yue DING Junfeng HE Ruofei GONG Hang DAI Xiong CHEN Yanan HUANG Zhi WANG pH Coupled Citric Acid Feeding Strategy to Promote Spore Production of Bacillus licheniformis Shipin gongye ke-ji bacillus licheniformis citric acid biomass spores qpcr |
| title | pH Coupled Citric Acid Feeding Strategy to Promote Spore Production of Bacillus licheniformis |
| title_full | pH Coupled Citric Acid Feeding Strategy to Promote Spore Production of Bacillus licheniformis |
| title_fullStr | pH Coupled Citric Acid Feeding Strategy to Promote Spore Production of Bacillus licheniformis |
| title_full_unstemmed | pH Coupled Citric Acid Feeding Strategy to Promote Spore Production of Bacillus licheniformis |
| title_short | pH Coupled Citric Acid Feeding Strategy to Promote Spore Production of Bacillus licheniformis |
| title_sort | ph coupled citric acid feeding strategy to promote spore production of bacillus licheniformis |
| topic | bacillus licheniformis citric acid biomass spores qpcr |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030261 |
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