The Abundance of the nifH Gene Became Higher and the nifH-Containing Diazotrophic Bacterial Communities Changed During Primary Succession in the Hailuogou Glacier Chronosequence, China
Primary successional ecosystems and the related soil development are often N limited. To date, N2-fixing communities during primary succession in alpine ecosystems have remained underexplored. In this study, we applied quantitative PCR (qPCR) quantitation and targeted amplicon sequencing of nifH in...
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| Language: | English |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.672656/full |
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| author | Yingyan Wang Yulan Chen Qinyu Xue Quanju Xiang Ke Zhao Xiumei Yu Qiang Chen Menggen Ma Hao Jiang Xiaoping Zhang Petri Penttinen Yunfu Gu |
| author_facet | Yingyan Wang Yulan Chen Qinyu Xue Quanju Xiang Ke Zhao Xiumei Yu Qiang Chen Menggen Ma Hao Jiang Xiaoping Zhang Petri Penttinen Yunfu Gu |
| author_sort | Yingyan Wang |
| collection | DOAJ |
| description | Primary successional ecosystems and the related soil development are often N limited. To date, N2-fixing communities during primary succession in alpine ecosystems have remained underexplored. In this study, we applied quantitative PCR (qPCR) quantitation and targeted amplicon sequencing of nifH in the Hailuogou Glacier foreland to investigate the succession of N2-fixing communities in five sites along a 62-year chronosequence. The abundance of the nifH gene increased along the primary succession in the chronosequence and correlated positively with pH, acetylene reduction activity, and water, organic C, total and available N, and available P contents. The increases in alpha diversity along the chronosequence may have been partly due to less competition for resources. In contrast to the clear separation based on soil properties, the changes in the diazotrophic community composition lacked a clear trend and were associated mostly with changes in soil available K and organic C contents. The changes among differentially abundant genera were possibly due to the changes in plant coverage and species composition. The whole primary succession of the diazotrophic communities was consistent with stochastic community assembly, which is indicative of low competitive pressure. |
| first_indexed | 2024-12-17T01:15:08Z |
| format | Article |
| id | doaj.art-e2aff4993adb4ea99ad35dd149756139 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T01:15:08Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e2aff4993adb4ea99ad35dd1497561392022-12-21T22:09:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-05-011210.3389/fmicb.2021.672656672656The Abundance of the nifH Gene Became Higher and the nifH-Containing Diazotrophic Bacterial Communities Changed During Primary Succession in the Hailuogou Glacier Chronosequence, ChinaYingyan Wang0Yulan Chen1Qinyu Xue2Quanju Xiang3Ke Zhao4Xiumei Yu5Qiang Chen6Menggen Ma7Hao Jiang8Xiaoping Zhang9Petri Penttinen10Yunfu Gu11Department of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaInstitute of Chengdu Mountain Hazards and Environment, Chinese Academy Sciences, Chengdu, ChinaDepartment of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaDepartment of Microbiology, College of Resource Sciences and Technology, Sichuan Agricultural University, Chengdu, ChinaPrimary successional ecosystems and the related soil development are often N limited. To date, N2-fixing communities during primary succession in alpine ecosystems have remained underexplored. In this study, we applied quantitative PCR (qPCR) quantitation and targeted amplicon sequencing of nifH in the Hailuogou Glacier foreland to investigate the succession of N2-fixing communities in five sites along a 62-year chronosequence. The abundance of the nifH gene increased along the primary succession in the chronosequence and correlated positively with pH, acetylene reduction activity, and water, organic C, total and available N, and available P contents. The increases in alpha diversity along the chronosequence may have been partly due to less competition for resources. In contrast to the clear separation based on soil properties, the changes in the diazotrophic community composition lacked a clear trend and were associated mostly with changes in soil available K and organic C contents. The changes among differentially abundant genera were possibly due to the changes in plant coverage and species composition. The whole primary succession of the diazotrophic communities was consistent with stochastic community assembly, which is indicative of low competitive pressure.https://www.frontiersin.org/articles/10.3389/fmicb.2021.672656/fullHailuogou Glacier chronosequenceprimary successionN2-fixing microorganismcommunity compositionstochastic processes |
| spellingShingle | Yingyan Wang Yulan Chen Qinyu Xue Quanju Xiang Ke Zhao Xiumei Yu Qiang Chen Menggen Ma Hao Jiang Xiaoping Zhang Petri Penttinen Yunfu Gu The Abundance of the nifH Gene Became Higher and the nifH-Containing Diazotrophic Bacterial Communities Changed During Primary Succession in the Hailuogou Glacier Chronosequence, China Frontiers in Microbiology Hailuogou Glacier chronosequence primary succession N2-fixing microorganism community composition stochastic processes |
| title | The Abundance of the nifH Gene Became Higher and the nifH-Containing Diazotrophic Bacterial Communities Changed During Primary Succession in the Hailuogou Glacier Chronosequence, China |
| title_full | The Abundance of the nifH Gene Became Higher and the nifH-Containing Diazotrophic Bacterial Communities Changed During Primary Succession in the Hailuogou Glacier Chronosequence, China |
| title_fullStr | The Abundance of the nifH Gene Became Higher and the nifH-Containing Diazotrophic Bacterial Communities Changed During Primary Succession in the Hailuogou Glacier Chronosequence, China |
| title_full_unstemmed | The Abundance of the nifH Gene Became Higher and the nifH-Containing Diazotrophic Bacterial Communities Changed During Primary Succession in the Hailuogou Glacier Chronosequence, China |
| title_short | The Abundance of the nifH Gene Became Higher and the nifH-Containing Diazotrophic Bacterial Communities Changed During Primary Succession in the Hailuogou Glacier Chronosequence, China |
| title_sort | abundance of the nifh gene became higher and the nifh containing diazotrophic bacterial communities changed during primary succession in the hailuogou glacier chronosequence china |
| topic | Hailuogou Glacier chronosequence primary succession N2-fixing microorganism community composition stochastic processes |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.672656/full |
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