Development and Validation of Multi-Residue Method for Drugs Analysis in Human Feces by Liquid Chromatography–Tandem Mass Spectrometry
The use of veterinary drugs in animal production is a common practice to secure animal and human health. However, residues of administrated drugs could be present in animal food products. Levels of drugs in food of animal origin are regulated within the European Union. In recent years, residues have...
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/5/1474 |
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| author | Gabriel Míguez-Suárez Alejandra Cardelle-Cobas Laura Sinisterra-Loaiza Beatriz Vázquez Alberto Cepeda Carolina Nebot |
| author_facet | Gabriel Míguez-Suárez Alejandra Cardelle-Cobas Laura Sinisterra-Loaiza Beatriz Vázquez Alberto Cepeda Carolina Nebot |
| author_sort | Gabriel Míguez-Suárez |
| collection | DOAJ |
| description | The use of veterinary drugs in animal production is a common practice to secure animal and human health. However, residues of administrated drugs could be present in animal food products. Levels of drugs in food of animal origin are regulated within the European Union. In recent years, residues have been detected not only in food, but also in the environmental elements such as water or soil, meaning that humans are involuntarily exposed to these substances. This article presents a multiclass method for the analysis of various therapeutic groups of pharmaceuticals in human feces. Pharmaceuticals are extracted from feces with an acid extraction solvent, and after filtration the extract was analyzed by HPLC–MS/MS. A limit of detection of 10 ng/g was achieved for 9 pharmaceuticals, with linearity over 0.99 and repeatability and reproducibility lower than 20%. The method was satisfactorily applied in 25 feces samples of individuals that had declared not to be under medical treatment for the last two months. Results indicate the presence of six different compounds at concentration between 10 and 456 ng/g. This preliminary study showed the involuntary exposure of human gut microbiota to active substances such as pharmaceuticals |
| first_indexed | 2024-03-09T20:30:35Z |
| format | Article |
| id | doaj.art-e2b1256206ca4c069bedfa51d137ccf5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T20:30:35Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e2b1256206ca4c069bedfa51d137ccf52023-11-23T23:24:53ZengMDPI AGMolecules1420-30492022-02-01275147410.3390/molecules27051474Development and Validation of Multi-Residue Method for Drugs Analysis in Human Feces by Liquid Chromatography–Tandem Mass SpectrometryGabriel Míguez-Suárez0Alejandra Cardelle-Cobas1Laura Sinisterra-Loaiza2Beatriz Vázquez3Alberto Cepeda4Carolina Nebot5Laboratory of Hygiene, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, 27002 Lugo, SpainLaboratory of Hygiene, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, 27002 Lugo, SpainLaboratory of Hygiene, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, 27002 Lugo, SpainLaboratory of Hygiene, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, 27002 Lugo, SpainLaboratory of Hygiene, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, 27002 Lugo, SpainLaboratory of Hygiene, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, Universidade de Santiago de Compostela, 27002 Lugo, SpainThe use of veterinary drugs in animal production is a common practice to secure animal and human health. However, residues of administrated drugs could be present in animal food products. Levels of drugs in food of animal origin are regulated within the European Union. In recent years, residues have been detected not only in food, but also in the environmental elements such as water or soil, meaning that humans are involuntarily exposed to these substances. This article presents a multiclass method for the analysis of various therapeutic groups of pharmaceuticals in human feces. Pharmaceuticals are extracted from feces with an acid extraction solvent, and after filtration the extract was analyzed by HPLC–MS/MS. A limit of detection of 10 ng/g was achieved for 9 pharmaceuticals, with linearity over 0.99 and repeatability and reproducibility lower than 20%. The method was satisfactorily applied in 25 feces samples of individuals that had declared not to be under medical treatment for the last two months. Results indicate the presence of six different compounds at concentration between 10 and 456 ng/g. This preliminary study showed the involuntary exposure of human gut microbiota to active substances such as pharmaceuticalshttps://www.mdpi.com/1420-3049/27/5/1474antibioticfood of animal originwaterfecesfoodHPLC–MS/MS |
| spellingShingle | Gabriel Míguez-Suárez Alejandra Cardelle-Cobas Laura Sinisterra-Loaiza Beatriz Vázquez Alberto Cepeda Carolina Nebot Development and Validation of Multi-Residue Method for Drugs Analysis in Human Feces by Liquid Chromatography–Tandem Mass Spectrometry Molecules antibiotic food of animal origin water feces food HPLC–MS/MS |
| title | Development and Validation of Multi-Residue Method for Drugs Analysis in Human Feces by Liquid Chromatography–Tandem Mass Spectrometry |
| title_full | Development and Validation of Multi-Residue Method for Drugs Analysis in Human Feces by Liquid Chromatography–Tandem Mass Spectrometry |
| title_fullStr | Development and Validation of Multi-Residue Method for Drugs Analysis in Human Feces by Liquid Chromatography–Tandem Mass Spectrometry |
| title_full_unstemmed | Development and Validation of Multi-Residue Method for Drugs Analysis in Human Feces by Liquid Chromatography–Tandem Mass Spectrometry |
| title_short | Development and Validation of Multi-Residue Method for Drugs Analysis in Human Feces by Liquid Chromatography–Tandem Mass Spectrometry |
| title_sort | development and validation of multi residue method for drugs analysis in human feces by liquid chromatography tandem mass spectrometry |
| topic | antibiotic food of animal origin water feces food HPLC–MS/MS |
| url | https://www.mdpi.com/1420-3049/27/5/1474 |
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