Folate (vitamin B9) content analysis in bread wheat (Triticum aestivum L.)
Vitamin B9, particularly folic acid, is an essential molecule for human health. Wheat flour is one of the major sources of calorie intake by humans. The selection of folate-rich genotypes in wheat breeding can enhance the natural folate value in the daily diet. This study used a precise, high-perfor...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.933358/full |
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| author | Jun Zheng Xingsu Wang Bangbang Wu Ling Qiao Jiajia Zhao Mohammad Pourkheirandish Juanling Wang Xingwei Zheng |
| author_facet | Jun Zheng Xingsu Wang Bangbang Wu Ling Qiao Jiajia Zhao Mohammad Pourkheirandish Juanling Wang Xingwei Zheng |
| author_sort | Jun Zheng |
| collection | DOAJ |
| description | Vitamin B9, particularly folic acid, is an essential molecule for human health. Wheat flour is one of the major sources of calorie intake by humans. The selection of folate-rich genotypes in wheat breeding can enhance the natural folate value in the daily diet. This study used a precise, high-performance liquid chromatography (HPLC) assay to analyze folate content in a 262-accession Chinese wheat mini-core collection (MCC) grown under three environments. Four folate derivatives in grains including tetrahydrofolate (THF), 5-methyltetrahydrofolate (5-CH3-THF), 5-formyltetrahydrofolate (5-CHO-THF), and 5,10-methenyltetrahydrofolate (5,10-CH+THF) were considered. An association analysis of water regimes, accession types, released years, geographical origin, and agronomic traits with folate content was conducted for the first time. There was a large amount of variation in folate content in the analyzed accessions, with genotype identified as the main influencing factor. Total folate content was significantly correlated with the content of the four MCC derivatives under the three environments. 5-CH3-THF and 5-CHO-THF were the most abundant among the four folate derivatives and were positively correlated with high folate content. The 12 accessions with the highest folate content showed an average of more than 80 μg/100 g. The analysis demonstrated that this Chinese wheat had not undergone extensive selection for folate content during breeding, which is unrelated to the geographical origin, accession types, winter/spring types, and grain colors of wheat. The content of THF, 5-CH3-THF, and 5,10-CH+THF was significantly negatively correlated with grain width, grain thickness, and thousand kernel weight. A relatively weak negative relationship manifested between folate contents and flowering date, whereas no significant correlation with tiller number, grain number per spike, maturity date, height, and spike length was detected. The investigation benefits wheat breeders for folate enhancement. |
| first_indexed | 2024-04-13T17:53:46Z |
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| id | doaj.art-e2b17c68b18e4356bf9e0257cc258c63 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-13T17:53:46Z |
| publishDate | 2022-10-01 |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-e2b17c68b18e4356bf9e0257cc258c632022-12-22T02:36:36ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-10-01910.3389/fnut.2022.933358933358Folate (vitamin B9) content analysis in bread wheat (Triticum aestivum L.)Jun Zheng0Xingsu Wang1Bangbang Wu2Ling Qiao3Jiajia Zhao4Mohammad Pourkheirandish5Juanling Wang6Xingwei Zheng7Institute of Wheat Research, Shanxi Agricultural University, Linfen, ChinaInstitute of Wheat Research, Shanxi Agricultural University, Linfen, ChinaInstitute of Wheat Research, Shanxi Agricultural University, Linfen, ChinaInstitute of Wheat Research, Shanxi Agricultural University, Linfen, ChinaInstitute of Wheat Research, Shanxi Agricultural University, Linfen, ChinaFaculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, VIC, AustraliaInstitute of Wheat Research, Shanxi Agricultural University, Linfen, ChinaInstitute of Wheat Research, Shanxi Agricultural University, Linfen, ChinaVitamin B9, particularly folic acid, is an essential molecule for human health. Wheat flour is one of the major sources of calorie intake by humans. The selection of folate-rich genotypes in wheat breeding can enhance the natural folate value in the daily diet. This study used a precise, high-performance liquid chromatography (HPLC) assay to analyze folate content in a 262-accession Chinese wheat mini-core collection (MCC) grown under three environments. Four folate derivatives in grains including tetrahydrofolate (THF), 5-methyltetrahydrofolate (5-CH3-THF), 5-formyltetrahydrofolate (5-CHO-THF), and 5,10-methenyltetrahydrofolate (5,10-CH+THF) were considered. An association analysis of water regimes, accession types, released years, geographical origin, and agronomic traits with folate content was conducted for the first time. There was a large amount of variation in folate content in the analyzed accessions, with genotype identified as the main influencing factor. Total folate content was significantly correlated with the content of the four MCC derivatives under the three environments. 5-CH3-THF and 5-CHO-THF were the most abundant among the four folate derivatives and were positively correlated with high folate content. The 12 accessions with the highest folate content showed an average of more than 80 μg/100 g. The analysis demonstrated that this Chinese wheat had not undergone extensive selection for folate content during breeding, which is unrelated to the geographical origin, accession types, winter/spring types, and grain colors of wheat. The content of THF, 5-CH3-THF, and 5,10-CH+THF was significantly negatively correlated with grain width, grain thickness, and thousand kernel weight. A relatively weak negative relationship manifested between folate contents and flowering date, whereas no significant correlation with tiller number, grain number per spike, maturity date, height, and spike length was detected. The investigation benefits wheat breeders for folate enhancement.https://www.frontiersin.org/articles/10.3389/fnut.2022.933358/fullwheatfolatesgenetic variationaccession typesagronomic traits |
| spellingShingle | Jun Zheng Xingsu Wang Bangbang Wu Ling Qiao Jiajia Zhao Mohammad Pourkheirandish Juanling Wang Xingwei Zheng Folate (vitamin B9) content analysis in bread wheat (Triticum aestivum L.) Frontiers in Nutrition wheat folates genetic variation accession types agronomic traits |
| title | Folate (vitamin B9) content analysis in bread wheat (Triticum aestivum L.) |
| title_full | Folate (vitamin B9) content analysis in bread wheat (Triticum aestivum L.) |
| title_fullStr | Folate (vitamin B9) content analysis in bread wheat (Triticum aestivum L.) |
| title_full_unstemmed | Folate (vitamin B9) content analysis in bread wheat (Triticum aestivum L.) |
| title_short | Folate (vitamin B9) content analysis in bread wheat (Triticum aestivum L.) |
| title_sort | folate vitamin b9 content analysis in bread wheat triticum aestivum l |
| topic | wheat folates genetic variation accession types agronomic traits |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.933358/full |
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