Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium <i>Rhodococcus</i> <i>erythropolis</i> AQ5-07 from Antarctica
With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/17/3878 |
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| author | Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkharnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad |
| author_facet | Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkharnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad |
| author_sort | Salihu Ibrahim |
| collection | DOAJ |
| description | With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, <i>Rhodococcus erythropolis</i> strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (<i>q<sub>max</sub></i>), concentration of substrate at the half maximum specific growth rate, <i>K<sub>s</sub></i>% (<i>v/v</i>) and the inhibition constant <i>K<sub>i</sub></i>% (<i>v/v</i>), with values of 0.142 h<sup>−1</sup>, 7.743% (<i>v/v</i>) and 0.399% (<i>v/v</i>), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. |
| first_indexed | 2024-03-10T16:50:14Z |
| format | Article |
| id | doaj.art-e2b2614a9797462cac84bc8768ae82e4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:50:14Z |
| publishDate | 2020-08-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e2b2614a9797462cac84bc8768ae82e42023-11-20T11:21:29ZengMDPI AGMolecules1420-30492020-08-012517387810.3390/molecules25173878Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium <i>Rhodococcus</i> <i>erythropolis</i> AQ5-07 from AntarcticaSalihu Ibrahim0Khalilah Abdul Khalil1Khadijah Nabilah Mohd Zahri2Claudio Gomez-Fuentes3Peter Convey4Azham Zulkharnain5Suriana Sabri6Siti Aisyah Alias7Gerardo González-Rocha8Siti Aqlima Ahmad9Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, MalaysiaSchool of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor 40450, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, MalaysiaDepartment of Chemical Engineering, Universidad de Magallanes, Avda. Bulnes 01855, Punta Arenas, Región de Magallanes y Antártica Chilena, ChileBritish Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 0ET, UKDepartment of Bioscience and Engineering, College of system Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama 337-8570, JapanDepartment of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, MalaysiaNational Antarctic Research Centre, B303 Level 3, Block B, IPS Building, Universiti Malaya, Kuala Lumpur 50603, MalaysiaLaboratorio de Investigacion en Agentes Antibacterianos, Facultad de Ciencias Biologicas, Universidad de Concepcion, Concepcion 4070386, ChileDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, MalaysiaWith the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, <i>Rhodococcus erythropolis</i> strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (<i>q<sub>max</sub></i>), concentration of substrate at the half maximum specific growth rate, <i>K<sub>s</sub></i>% (<i>v/v</i>) and the inhibition constant <i>K<sub>i</sub></i>% (<i>v/v</i>), with values of 0.142 h<sup>−1</sup>, 7.743% (<i>v/v</i>) and 0.399% (<i>v/v</i>), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions.https://www.mdpi.com/1420-3049/25/17/3878Antarcticabiosurfactantscanola oilkineticshaldanemodelling |
| spellingShingle | Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkharnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium <i>Rhodococcus</i> <i>erythropolis</i> AQ5-07 from Antarctica Molecules Antarctica biosurfactants canola oil kinetics haldane modelling |
| title | Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium <i>Rhodococcus</i> <i>erythropolis</i> AQ5-07 from Antarctica |
| title_full | Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium <i>Rhodococcus</i> <i>erythropolis</i> AQ5-07 from Antarctica |
| title_fullStr | Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium <i>Rhodococcus</i> <i>erythropolis</i> AQ5-07 from Antarctica |
| title_full_unstemmed | Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium <i>Rhodococcus</i> <i>erythropolis</i> AQ5-07 from Antarctica |
| title_short | Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium <i>Rhodococcus</i> <i>erythropolis</i> AQ5-07 from Antarctica |
| title_sort | biosurfactant production and growth kinetics studies of the waste canola oil degrading bacterium i rhodococcus i i erythropolis i aq5 07 from antarctica |
| topic | Antarctica biosurfactants canola oil kinetics haldane modelling |
| url | https://www.mdpi.com/1420-3049/25/17/3878 |
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