Assessment of acetylcholinesterase activity in CD9-positive exosomes from patients with Parkinson’s disease
IntroductionParkinson’s disease (PD) is a neurodegenerative disorder characterized by dopaminergic dysfunction and associated with abnormalities in the cholinergic system. However, the relationship between PD and cholinergic dysfunction, particularly in exosomes, is not fully understood.MethodsWe en...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-02-01
|
| Series: | Frontiers in Aging Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnagi.2024.1332455/full |
| _version_ | 1797322525980491776 |
|---|---|
| author | Sumin Jeong Sumin Jeong Kyu Hwan Shim Danyeong Kim Danyeong Kim Heewon Bae Da-Eun Jeong Min Ju Kang Seong Soo A. An |
| author_facet | Sumin Jeong Sumin Jeong Kyu Hwan Shim Danyeong Kim Danyeong Kim Heewon Bae Da-Eun Jeong Min Ju Kang Seong Soo A. An |
| author_sort | Sumin Jeong |
| collection | DOAJ |
| description | IntroductionParkinson’s disease (PD) is a neurodegenerative disorder characterized by dopaminergic dysfunction and associated with abnormalities in the cholinergic system. However, the relationship between PD and cholinergic dysfunction, particularly in exosomes, is not fully understood.MethodsWe enrolled 37 patients with PD and 44 healthy controls (HC) to investigate acetylcholinesterase (AChE) activity in CD9-positive and L1CAM-positive exosomes. Exosomes were isolated from plasma using antibody-coupled magnetic beads, and their sizes and concentrations were assessed using transmission electron microscopy, nanoparticle tracking analysis, and western blotting. Subsequently, the AChE activity in these exosomes was analyzed in relation to various clinical parameters.ResultsA significant decrease in AChE activity was observed in CD9-positive exosomes derived from patients with PD, whereas no significant differences were found in L1CAM-positive exosomes. Further analysis with a larger sample size confirmed a substantial reduction in AChE activity in CD9-positive exosomes from the PD plasma, with moderate diagnostic accuracy. The decrease in AChE activity of CD9-positive exosomes did not show an association with cognitive impairment but displayed a trend toward correlation with PD progression.DiscussionThe reduction in AChE activity in CD9-positive exosomes suggests potential peripheral cholinergic dysfunction in PD, independent of the central cholinergic system. The observed alterations in AChE activity provide valuable insights into the association between cholinergic dysfunction and the pathogenesis of PD. |
| first_indexed | 2024-03-08T05:15:33Z |
| format | Article |
| id | doaj.art-e2b46e49284a4e2d99da1c32898918c2 |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-03-08T05:15:33Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-e2b46e49284a4e2d99da1c32898918c22024-02-07T04:36:42ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652024-02-011610.3389/fnagi.2024.13324551332455Assessment of acetylcholinesterase activity in CD9-positive exosomes from patients with Parkinson’s diseaseSumin Jeong0Sumin Jeong1Kyu Hwan Shim2Danyeong Kim3Danyeong Kim4Heewon Bae5Da-Eun Jeong6Min Ju Kang7Seong Soo A. An8Department of Bionano Technology, Gachon University, Seongnam, Republic of KoreaDepartment of Neurology, Veterans Health Service Medical Center, Veterans Medical Research Institute, Seoul, Republic of KoreaDepartment of Bionano Technology, Gachon University, Seongnam, Republic of KoreaDepartment of Bionano Technology, Gachon University, Seongnam, Republic of KoreaDepartment of Neurology, Veterans Health Service Medical Center, Veterans Medical Research Institute, Seoul, Republic of KoreaDepartment of Neurology, Veterans Health Service Medical Center, Veterans Medical Research Institute, Seoul, Republic of KoreaDepartment of Neurology, Veterans Health Service Medical Center, Veterans Medical Research Institute, Seoul, Republic of KoreaDepartment of Neurology, Veterans Health Service Medical Center, Veterans Medical Research Institute, Seoul, Republic of KoreaDepartment of Bionano Technology, Gachon University, Seongnam, Republic of KoreaIntroductionParkinson’s disease (PD) is a neurodegenerative disorder characterized by dopaminergic dysfunction and associated with abnormalities in the cholinergic system. However, the relationship between PD and cholinergic dysfunction, particularly in exosomes, is not fully understood.MethodsWe enrolled 37 patients with PD and 44 healthy controls (HC) to investigate acetylcholinesterase (AChE) activity in CD9-positive and L1CAM-positive exosomes. Exosomes were isolated from plasma using antibody-coupled magnetic beads, and their sizes and concentrations were assessed using transmission electron microscopy, nanoparticle tracking analysis, and western blotting. Subsequently, the AChE activity in these exosomes was analyzed in relation to various clinical parameters.ResultsA significant decrease in AChE activity was observed in CD9-positive exosomes derived from patients with PD, whereas no significant differences were found in L1CAM-positive exosomes. Further analysis with a larger sample size confirmed a substantial reduction in AChE activity in CD9-positive exosomes from the PD plasma, with moderate diagnostic accuracy. The decrease in AChE activity of CD9-positive exosomes did not show an association with cognitive impairment but displayed a trend toward correlation with PD progression.DiscussionThe reduction in AChE activity in CD9-positive exosomes suggests potential peripheral cholinergic dysfunction in PD, independent of the central cholinergic system. The observed alterations in AChE activity provide valuable insights into the association between cholinergic dysfunction and the pathogenesis of PD.https://www.frontiersin.org/articles/10.3389/fnagi.2024.1332455/fullParkinson’s diseasebloodacetylcholinesteraseexosomesCD9cholinergic dysfunction |
| spellingShingle | Sumin Jeong Sumin Jeong Kyu Hwan Shim Danyeong Kim Danyeong Kim Heewon Bae Da-Eun Jeong Min Ju Kang Seong Soo A. An Assessment of acetylcholinesterase activity in CD9-positive exosomes from patients with Parkinson’s disease Frontiers in Aging Neuroscience Parkinson’s disease blood acetylcholinesterase exosomes CD9 cholinergic dysfunction |
| title | Assessment of acetylcholinesterase activity in CD9-positive exosomes from patients with Parkinson’s disease |
| title_full | Assessment of acetylcholinesterase activity in CD9-positive exosomes from patients with Parkinson’s disease |
| title_fullStr | Assessment of acetylcholinesterase activity in CD9-positive exosomes from patients with Parkinson’s disease |
| title_full_unstemmed | Assessment of acetylcholinesterase activity in CD9-positive exosomes from patients with Parkinson’s disease |
| title_short | Assessment of acetylcholinesterase activity in CD9-positive exosomes from patients with Parkinson’s disease |
| title_sort | assessment of acetylcholinesterase activity in cd9 positive exosomes from patients with parkinson s disease |
| topic | Parkinson’s disease blood acetylcholinesterase exosomes CD9 cholinergic dysfunction |
| url | https://www.frontiersin.org/articles/10.3389/fnagi.2024.1332455/full |
| work_keys_str_mv | AT suminjeong assessmentofacetylcholinesteraseactivityincd9positiveexosomesfrompatientswithparkinsonsdisease AT suminjeong assessmentofacetylcholinesteraseactivityincd9positiveexosomesfrompatientswithparkinsonsdisease AT kyuhwanshim assessmentofacetylcholinesteraseactivityincd9positiveexosomesfrompatientswithparkinsonsdisease AT danyeongkim assessmentofacetylcholinesteraseactivityincd9positiveexosomesfrompatientswithparkinsonsdisease AT danyeongkim assessmentofacetylcholinesteraseactivityincd9positiveexosomesfrompatientswithparkinsonsdisease AT heewonbae assessmentofacetylcholinesteraseactivityincd9positiveexosomesfrompatientswithparkinsonsdisease AT daeunjeong assessmentofacetylcholinesteraseactivityincd9positiveexosomesfrompatientswithparkinsonsdisease AT minjukang assessmentofacetylcholinesteraseactivityincd9positiveexosomesfrompatientswithparkinsonsdisease AT seongsooaan assessmentofacetylcholinesteraseactivityincd9positiveexosomesfrompatientswithparkinsonsdisease |