Induction of Different Apoptosis Pathways by Two Proteusmirabilis Clinical Isolates Strains in Prostatic Epithelial Cells
Bacterial prostatitis is believed to be the leading cause of recurrent urinary tract infections (UTIs) in men under 50 years of age and occurs both as an acute febrile disease responsive to antibiotics and as a chronic infection that is often unresponsive to antibiotic treatment. Proteusmirabilis is...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.01855/full |
| _version_ | 1811254577483218944 |
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| author | Alessandra Fusco Vittoria Savio Anna De Filippis Antonio Tufano Giovanna Donnarumma |
| author_facet | Alessandra Fusco Vittoria Savio Anna De Filippis Antonio Tufano Giovanna Donnarumma |
| author_sort | Alessandra Fusco |
| collection | DOAJ |
| description | Bacterial prostatitis is believed to be the leading cause of recurrent urinary tract infections (UTIs) in men under 50 years of age and occurs both as an acute febrile disease responsive to antibiotics and as a chronic infection that is often unresponsive to antibiotic treatment. Proteusmirabilis is more commonly associated with UTIs in these abnormalities, especially in patients undergoing catheterisation. This pathogen is able to colonise the host’s tissues and to cause disease thanks to the production of many virulence factors such as fimbriae, flagella, immune avoidance, host-damaging factors, and the ability to form crystalline biofilms. In addition, Proteus lipid A may exhibit apoptotic activity and induce desquamation of epithelial cells. The aim of this work was to evaluate the ability of two clinically isolated strains of P. mirabilis that are phenotypically different, named PM1 of PM2, respectively, to induce apoptosis in human prostatic adenocarcinoma PC-3. Our results demonstrate that PM1 and PM2 are able to activate two different apoptotic pathways, and this different behaviour is confirmed by the expression level of the ZapA gene, molecular fingerprinting and different spectrum of antibiotic resistance. The identification and knowledge of relations between the microorganism and host may provide the basis for new solutions to clinical problems with regard to diagnosis and therapy. |
| first_indexed | 2024-04-12T17:09:38Z |
| format | Article |
| id | doaj.art-e2b84f766f624682984bec22b93cdb14 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-12T17:09:38Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-e2b84f766f624682984bec22b93cdb142022-12-22T03:23:50ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-12-01910.3389/fphys.2018.01855386683Induction of Different Apoptosis Pathways by Two Proteusmirabilis Clinical Isolates Strains in Prostatic Epithelial CellsAlessandra Fusco0Vittoria Savio1Anna De Filippis2Antonio Tufano3Giovanna Donnarumma4Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania Luigi Vanvitelli, Naples, ItalyDepartment of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania Luigi Vanvitelli, Naples, ItalyDepartment of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania Luigi Vanvitelli, Naples, ItalyUrology School, Sapienza University of Rome, Rome, ItalyDepartment of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania Luigi Vanvitelli, Naples, ItalyBacterial prostatitis is believed to be the leading cause of recurrent urinary tract infections (UTIs) in men under 50 years of age and occurs both as an acute febrile disease responsive to antibiotics and as a chronic infection that is often unresponsive to antibiotic treatment. Proteusmirabilis is more commonly associated with UTIs in these abnormalities, especially in patients undergoing catheterisation. This pathogen is able to colonise the host’s tissues and to cause disease thanks to the production of many virulence factors such as fimbriae, flagella, immune avoidance, host-damaging factors, and the ability to form crystalline biofilms. In addition, Proteus lipid A may exhibit apoptotic activity and induce desquamation of epithelial cells. The aim of this work was to evaluate the ability of two clinically isolated strains of P. mirabilis that are phenotypically different, named PM1 of PM2, respectively, to induce apoptosis in human prostatic adenocarcinoma PC-3. Our results demonstrate that PM1 and PM2 are able to activate two different apoptotic pathways, and this different behaviour is confirmed by the expression level of the ZapA gene, molecular fingerprinting and different spectrum of antibiotic resistance. The identification and knowledge of relations between the microorganism and host may provide the basis for new solutions to clinical problems with regard to diagnosis and therapy.https://www.frontiersin.org/article/10.3389/fphys.2018.01855/fullProteusUTIsprostatitisapoptosisantibiotic resistance |
| spellingShingle | Alessandra Fusco Vittoria Savio Anna De Filippis Antonio Tufano Giovanna Donnarumma Induction of Different Apoptosis Pathways by Two Proteusmirabilis Clinical Isolates Strains in Prostatic Epithelial Cells Frontiers in Physiology Proteus UTIs prostatitis apoptosis antibiotic resistance |
| title | Induction of Different Apoptosis Pathways by Two Proteusmirabilis Clinical Isolates Strains in Prostatic Epithelial Cells |
| title_full | Induction of Different Apoptosis Pathways by Two Proteusmirabilis Clinical Isolates Strains in Prostatic Epithelial Cells |
| title_fullStr | Induction of Different Apoptosis Pathways by Two Proteusmirabilis Clinical Isolates Strains in Prostatic Epithelial Cells |
| title_full_unstemmed | Induction of Different Apoptosis Pathways by Two Proteusmirabilis Clinical Isolates Strains in Prostatic Epithelial Cells |
| title_short | Induction of Different Apoptosis Pathways by Two Proteusmirabilis Clinical Isolates Strains in Prostatic Epithelial Cells |
| title_sort | induction of different apoptosis pathways by two proteusmirabilis clinical isolates strains in prostatic epithelial cells |
| topic | Proteus UTIs prostatitis apoptosis antibiotic resistance |
| url | https://www.frontiersin.org/article/10.3389/fphys.2018.01855/full |
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