Lipid transfer from plants to arbuscular mycorrhiza fungi
Arbuscular mycorrhiza (AM) symbioses contribute to global carbon cycles as plant hosts divert up to 20% of photosynthate to the obligate biotrophic fungi. Previous studies suggested carbohydrates as the only form of carbon transferred to the fungi. However, de novo fatty acid (FA) synthesis has not...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-07-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/29107 |
| _version_ | 1811253401563955200 |
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| author | Andreas Keymer Priya Pimprikar Vera Wewer Claudia Huber Mathias Brands Simone L Bucerius Pierre-Marc Delaux Verena Klingl Edda von Röpenack-Lahaye Trevor L Wang Wolfgang Eisenreich Peter Dörmann Martin Parniske Caroline Gutjahr |
| author_facet | Andreas Keymer Priya Pimprikar Vera Wewer Claudia Huber Mathias Brands Simone L Bucerius Pierre-Marc Delaux Verena Klingl Edda von Röpenack-Lahaye Trevor L Wang Wolfgang Eisenreich Peter Dörmann Martin Parniske Caroline Gutjahr |
| author_sort | Andreas Keymer |
| collection | DOAJ |
| description | Arbuscular mycorrhiza (AM) symbioses contribute to global carbon cycles as plant hosts divert up to 20% of photosynthate to the obligate biotrophic fungi. Previous studies suggested carbohydrates as the only form of carbon transferred to the fungi. However, de novo fatty acid (FA) synthesis has not been observed in AM fungi in absence of the plant. In a forward genetic approach, we identified two Lotus japonicus mutants defective in AM-specific paralogs of lipid biosynthesis genes (KASI and GPAT6). These mutants perturb fungal development and accumulation of emblematic fungal 16:1ω5 FAs. Using isotopolog profiling we demonstrate that 13C patterns of fungal FAs recapitulate those of wild-type hosts, indicating cross-kingdom lipid transfer from plants to fungi. This transfer of labelled FAs was not observed for the AM-specific lipid biosynthesis mutants. Thus, growth and development of beneficial AM fungi is not only fueled by sugars but depends on lipid transfer from plant hosts. |
| first_indexed | 2024-04-12T16:50:22Z |
| format | Article |
| id | doaj.art-e2b8c66090bd44cd887b9c37312f974a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:50:22Z |
| publishDate | 2017-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e2b8c66090bd44cd887b9c37312f974a2022-12-22T03:24:26ZengeLife Sciences Publications LtdeLife2050-084X2017-07-01610.7554/eLife.29107Lipid transfer from plants to arbuscular mycorrhiza fungiAndreas Keymer0https://orcid.org/0000-0001-9933-3662Priya Pimprikar1Vera Wewer2Claudia Huber3Mathias Brands4https://orcid.org/0000-0001-6548-1448Simone L Bucerius5Pierre-Marc Delaux6https://orcid.org/0000-0002-6211-157XVerena Klingl7Edda von Röpenack-Lahaye8Trevor L Wang9Wolfgang Eisenreich10Peter Dörmann11https://orcid.org/0000-0002-5845-9370Martin Parniske12https://orcid.org/0000-0001-8561-747XCaroline Gutjahr13https://orcid.org/0000-0001-6163-745XFaculty of Biology, Genetics, LMU Munich, Biocenter Martinsried, Munich, GermanyFaculty of Biology, Genetics, LMU Munich, Biocenter Martinsried, Munich, GermanyInstitute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Bonn, GermanyBiochemistry, Technical University Munich, Garching, GermanyInstitute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Bonn, GermanyFaculty of Biology, Genetics, LMU Munich, Biocenter Martinsried, Munich, GermanyLaboratoire de Recherche en Sciences Végétale, Centre National de la Recherche Scientifique, Toulouse, FranceFaculty of Biology, Genetics, LMU Munich, Biocenter Martinsried, Munich, GermanyFaculty of Biology, Plant Sciences, LMU Munich, Biocenter Martinsried, Munich, GermanyJohn Innes Centre, Norwich Research Park, Norwich, United KingdomBiochemistry, Technical University Munich, Garching, GermanyInstitute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Bonn, GermanyFaculty of Biology, Genetics, LMU Munich, Biocenter Martinsried, Munich, GermanyFaculty of Biology, Genetics, LMU Munich, Biocenter Martinsried, Munich, GermanyArbuscular mycorrhiza (AM) symbioses contribute to global carbon cycles as plant hosts divert up to 20% of photosynthate to the obligate biotrophic fungi. Previous studies suggested carbohydrates as the only form of carbon transferred to the fungi. However, de novo fatty acid (FA) synthesis has not been observed in AM fungi in absence of the plant. In a forward genetic approach, we identified two Lotus japonicus mutants defective in AM-specific paralogs of lipid biosynthesis genes (KASI and GPAT6). These mutants perturb fungal development and accumulation of emblematic fungal 16:1ω5 FAs. Using isotopolog profiling we demonstrate that 13C patterns of fungal FAs recapitulate those of wild-type hosts, indicating cross-kingdom lipid transfer from plants to fungi. This transfer of labelled FAs was not observed for the AM-specific lipid biosynthesis mutants. Thus, growth and development of beneficial AM fungi is not only fueled by sugars but depends on lipid transfer from plant hosts.https://elifesciences.org/articles/29107Lotus japonicusRhizophagus irregularisarbuscular mycorrhizaroot symbiosislipids |
| spellingShingle | Andreas Keymer Priya Pimprikar Vera Wewer Claudia Huber Mathias Brands Simone L Bucerius Pierre-Marc Delaux Verena Klingl Edda von Röpenack-Lahaye Trevor L Wang Wolfgang Eisenreich Peter Dörmann Martin Parniske Caroline Gutjahr Lipid transfer from plants to arbuscular mycorrhiza fungi eLife Lotus japonicus Rhizophagus irregularis arbuscular mycorrhiza root symbiosis lipids |
| title | Lipid transfer from plants to arbuscular mycorrhiza fungi |
| title_full | Lipid transfer from plants to arbuscular mycorrhiza fungi |
| title_fullStr | Lipid transfer from plants to arbuscular mycorrhiza fungi |
| title_full_unstemmed | Lipid transfer from plants to arbuscular mycorrhiza fungi |
| title_short | Lipid transfer from plants to arbuscular mycorrhiza fungi |
| title_sort | lipid transfer from plants to arbuscular mycorrhiza fungi |
| topic | Lotus japonicus Rhizophagus irregularis arbuscular mycorrhiza root symbiosis lipids |
| url | https://elifesciences.org/articles/29107 |
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