Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychography
Kidney microscopy is a mainstay in studying the morphological structure, physiology and pathology of kidney tissues, as histology provides important results for a reliable diagnosis. A microscopy modality providing at same time high-resolution images and a wide field of view could be very useful for...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1120099/full |
| _version_ | 1828034660464066560 |
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| author | Marika Valentino Marika Valentino Vittorio Bianco Lisa Miccio Pasquale Memmolo Valentina Brancato Paolo Libretti Marcello Gambacorta Marco Salvatore Pietro Ferraro |
| author_facet | Marika Valentino Marika Valentino Vittorio Bianco Lisa Miccio Pasquale Memmolo Valentina Brancato Paolo Libretti Marcello Gambacorta Marco Salvatore Pietro Ferraro |
| author_sort | Marika Valentino |
| collection | DOAJ |
| description | Kidney microscopy is a mainstay in studying the morphological structure, physiology and pathology of kidney tissues, as histology provides important results for a reliable diagnosis. A microscopy modality providing at same time high-resolution images and a wide field of view could be very useful for analyzing the whole architecture and the functioning of the renal tissue. Recently, Fourier Ptychography (FP) has been proofed to yield images of biology samples such as tissues and in vitro cells while providing high resolution and large field of view, thus making it a unique and attractive opportunity for histopathology. Moreover, FP offers tissue imaging with high contrast assuring visualization of small desirable features, although with a stain-free mode that avoids any chemical process in histopathology. Here we report an experimental measuring campaign for creating the first comprehensive and extensive collection of images of kidney tissues captured by this FP microscope. We show that FP microscopy unlocks a new opportunity for the physicians to observe and judge renal tissue slides through the novel FP quantitative phase-contrast microscopy. Phase-contrast images of kidney tissue are analyzed by comparing them with the corresponding renal images taken under a conventional bright-field microscope both for stained and unstained tissue samples of different thicknesses. In depth discussion on the advantages and limitations of this new stain-free microscopy modality is reported, showing its usefulness over the classical light microscopy and opening a potential route for using FP in clinical practice for histopathology of kidney. |
| first_indexed | 2024-04-10T15:36:06Z |
| format | Article |
| id | doaj.art-e2bdbfe647314da183a5f6f3ea0f6e6f |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-10T15:36:06Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-e2bdbfe647314da183a5f6f3ea0f6e6f2023-02-13T06:53:59ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-02-011410.3389/fphys.2023.11200991120099Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychographyMarika Valentino0Marika Valentino1Vittorio Bianco2Lisa Miccio3Pasquale Memmolo4Valentina Brancato5Paolo Libretti6Marcello Gambacorta7Marco Salvatore8Pietro Ferraro9National Research Council (CNR) of Italy, Institute of Applied Sciences and Intelligent Systems (ISASI), Pozzuoli, ItalyDepartment of Electric and Information Technologies Engineering, University of Naples “Federico II”, Naples, ItalyNational Research Council (CNR) of Italy, Institute of Applied Sciences and Intelligent Systems (ISASI), Pozzuoli, ItalyNational Research Council (CNR) of Italy, Institute of Applied Sciences and Intelligent Systems (ISASI), Pozzuoli, ItalyNational Research Council (CNR) of Italy, Institute of Applied Sciences and Intelligent Systems (ISASI), Pozzuoli, ItalyIRCCS SYNLAB SDN, Naples, ItalyIRCCS SYNLAB SDN, Naples, ItalyIRCCS SYNLAB SDN, Naples, ItalyIRCCS SYNLAB SDN, Naples, ItalyNational Research Council (CNR) of Italy, Institute of Applied Sciences and Intelligent Systems (ISASI), Pozzuoli, ItalyKidney microscopy is a mainstay in studying the morphological structure, physiology and pathology of kidney tissues, as histology provides important results for a reliable diagnosis. A microscopy modality providing at same time high-resolution images and a wide field of view could be very useful for analyzing the whole architecture and the functioning of the renal tissue. Recently, Fourier Ptychography (FP) has been proofed to yield images of biology samples such as tissues and in vitro cells while providing high resolution and large field of view, thus making it a unique and attractive opportunity for histopathology. Moreover, FP offers tissue imaging with high contrast assuring visualization of small desirable features, although with a stain-free mode that avoids any chemical process in histopathology. Here we report an experimental measuring campaign for creating the first comprehensive and extensive collection of images of kidney tissues captured by this FP microscope. We show that FP microscopy unlocks a new opportunity for the physicians to observe and judge renal tissue slides through the novel FP quantitative phase-contrast microscopy. Phase-contrast images of kidney tissue are analyzed by comparing them with the corresponding renal images taken under a conventional bright-field microscope both for stained and unstained tissue samples of different thicknesses. In depth discussion on the advantages and limitations of this new stain-free microscopy modality is reported, showing its usefulness over the classical light microscopy and opening a potential route for using FP in clinical practice for histopathology of kidney.https://www.frontiersin.org/articles/10.3389/fphys.2023.1120099/fullrenal tissue morphologyfourier ptychographic microscopyunstained morphologyquantitative phase imagingkidney physiology |
| spellingShingle | Marika Valentino Marika Valentino Vittorio Bianco Lisa Miccio Pasquale Memmolo Valentina Brancato Paolo Libretti Marcello Gambacorta Marco Salvatore Pietro Ferraro Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychography Frontiers in Physiology renal tissue morphology fourier ptychographic microscopy unstained morphology quantitative phase imaging kidney physiology |
| title | Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychography |
| title_full | Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychography |
| title_fullStr | Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychography |
| title_full_unstemmed | Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychography |
| title_short | Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychography |
| title_sort | beyond conventional microscopy observing kidney tissues by means of fourier ptychography |
| topic | renal tissue morphology fourier ptychographic microscopy unstained morphology quantitative phase imaging kidney physiology |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1120099/full |
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