Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960
Tuberculosis is caused by Mycobacterium tuberculosis (M.tb) as well as Non-tubercular mycobacterium (NTM) with similar clinical presentation. Infections due to NTM are reported to have increased in the past few years. Growth of M.tb is inhibited by p-Nitrobenzoic acid (PNB), whereas, NTM are resista...
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| Format: | Article |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2010-07-01
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| Series: | Journal of Laboratory Physicians |
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| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.72157 |
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| author | Babita Sharma Nita Pal Bharti Malhotra Leela Vyas |
| author_facet | Babita Sharma Nita Pal Bharti Malhotra Leela Vyas |
| author_sort | Babita Sharma |
| collection | DOAJ |
| description | Tuberculosis is caused by Mycobacterium tuberculosis (M.tb) as well as Non-tubercular mycobacterium (NTM) with similar clinical presentation. Infections due to NTM are reported to have increased in the past few years. Growth of M.tb is inhibited by p-Nitrobenzoic acid (PNB), whereas, NTM are resistant. One hundred and nine isolates from various clinical samples were identified up to species level by their growth rate, pigmentation, and a battery of biochemical tests, including niacin accumulation, nitrate reduction, and heat-stable catalase (68°C) reactions. Para-nitrobenzoic acid (PNB) inhibition test was performed to differentiate between M.tb and NTM. PNB was added to the Lowenstein-Jensen (LJ) medium and BACTECTM MIGIT (Mycobacteria Growth Indicator Tube)960 medium to a final concentration of 500 μg/ml. All the M.tb isolates, including Mycobacterium tuberculosis H37Rv (standard strain), were inhibited by PNB on both LJ and MGIT 960. Of the NTM isolates, all were resistant to PNB on MGIT 960 and on LJ PNB, except one isolate of Mycobacterium marinum that was resistant to MGIT 960 PNB, but was susceptible to LJ PNB. The reporting time for M.tb ranged from 4–11 days (median 5.9 days) by MGIT 960 and for NTM it was 2–10 days with an average of 4.5 days. This study was carried out to establish the accuracy and efficiency of MGIT 960 PNB and to differentiate between M.tb and NTM. |
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| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-22T19:47:32Z |
| publishDate | 2010-07-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-e2bf2cd5c18d42bbb2ab006816ae0bf62022-12-21T18:14:39ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262010-07-0120208909210.4103/0974-2727.72157Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960Babita Sharma0Nita Pal1Bharti Malhotra2Leela Vyas3Department of Microbiology, SMS Medical College, Jaipur, IndiaDepartment of Microbiology, SMS Medical College, Jaipur, IndiaDepartment of Microbiology, SMS Medical College, Jaipur, IndiaDepartment of Microbiology, SMS Medical College, Jaipur, IndiaTuberculosis is caused by Mycobacterium tuberculosis (M.tb) as well as Non-tubercular mycobacterium (NTM) with similar clinical presentation. Infections due to NTM are reported to have increased in the past few years. Growth of M.tb is inhibited by p-Nitrobenzoic acid (PNB), whereas, NTM are resistant. One hundred and nine isolates from various clinical samples were identified up to species level by their growth rate, pigmentation, and a battery of biochemical tests, including niacin accumulation, nitrate reduction, and heat-stable catalase (68°C) reactions. Para-nitrobenzoic acid (PNB) inhibition test was performed to differentiate between M.tb and NTM. PNB was added to the Lowenstein-Jensen (LJ) medium and BACTECTM MIGIT (Mycobacteria Growth Indicator Tube)960 medium to a final concentration of 500 μg/ml. All the M.tb isolates, including Mycobacterium tuberculosis H37Rv (standard strain), were inhibited by PNB on both LJ and MGIT 960. Of the NTM isolates, all were resistant to PNB on MGIT 960 and on LJ PNB, except one isolate of Mycobacterium marinum that was resistant to MGIT 960 PNB, but was susceptible to LJ PNB. The reporting time for M.tb ranged from 4–11 days (median 5.9 days) by MGIT 960 and for NTM it was 2–10 days with an average of 4.5 days. This study was carried out to establish the accuracy and efficiency of MGIT 960 PNB and to differentiate between M.tb and NTM.http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.72157mgit960 mycobacterium tuberculosis non-tubercular mycobacterium (ntm)p-nitro-benzoic acid |
| spellingShingle | Babita Sharma Nita Pal Bharti Malhotra Leela Vyas Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960 Journal of Laboratory Physicians mgit960 mycobacterium tuberculosis non-tubercular mycobacterium (ntm) p-nitro-benzoic acid |
| title | Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960 |
| title_full | Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960 |
| title_fullStr | Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960 |
| title_full_unstemmed | Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960 |
| title_short | Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960 |
| title_sort | evaluation of a rapid differentiation test for mycobacterium tuberculosis from other mycobacteria by selective inhibition with p nitrobenzoic acid using mgit 960 |
| topic | mgit960 mycobacterium tuberculosis non-tubercular mycobacterium (ntm) p-nitro-benzoic acid |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.72157 |
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