Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus
Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms in different geographical areas. Little is known about the mechanism of liver damage and type of local immune response by HEV in swine. Therefore, the aim of this study was to determine the morphological...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2021-12-01
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| Series: | Veterinaria Italiana |
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| Online Access: | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1813 |
| _version_ | 1828217855890423808 |
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| author | Branislav Kureljušić Sanja Aleksić-Kovačević Božidar Savić Radiša Prodanović Nemanja Jezdimirović Vesna Milićević Jelena Maksimović-Zorić Jasna Kureljušić Jadranka Žutić Đorđe Knežević Ljiljana Spalević Vladimir Kukolj |
| author_facet | Branislav Kureljušić Sanja Aleksić-Kovačević Božidar Savić Radiša Prodanović Nemanja Jezdimirović Vesna Milićević Jelena Maksimović-Zorić Jasna Kureljušić Jadranka Žutić Đorđe Knežević Ljiljana Spalević Vladimir Kukolj |
| author_sort | Branislav Kureljušić |
| collection | DOAJ |
| description |
Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms in different geographical areas. Little is known about the mechanism of liver damage and type of local immune response by HEV in swine. Therefore, the aim of this study was to determine the morphological and immunophenotypic characteristics of hepatic lesions caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12 slaughtered 10 weeks old pigs which were RT-PCR positive for HEV RNA in rectal swab samples have been used. Livers were macroscopically examined and samples were taken for histopathological, immunohistochemical (CD3, CD79α and TGF-β1), semiquantitative, morphometric analysis, RT-nested-PCR, PCR and bacteriological analysis. Microscopically, mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically, portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly CD3+ T cells (234.80 ± 79.98). An intense TGF-β1 positive reaction was observed within the mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT-nested-PCR. HEV is noncytopathic, and this finding provides further evidence for an immune mediated pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte damage is clearly demonstrated.
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| first_indexed | 2024-04-12T15:54:32Z |
| format | Article |
| id | doaj.art-e2c0b3e22eff4147b534dfcd3648afe3 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-04-12T15:54:32Z |
| publishDate | 2021-12-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-e2c0b3e22eff4147b534dfcd3648afe32022-12-22T03:26:25ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272021-12-0157410.12834/VetIt.1813.9553.3Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virusBranislav Kureljušić0Sanja Aleksić-Kovačević1Božidar Savić2Radiša Prodanović3Nemanja Jezdimirović4Vesna Milićević5Jelena Maksimović-Zorić6Jasna Kureljušić7Jadranka Žutić8Đorđe Knežević9Ljiljana Spalević10Vladimir Kukolj11epartment of Pathology, Institute of Veterinary Medicine of Serbia, Belgrade, Serbiaepartment of Pathology, Faculty of Veterinary Medicine, University of Belgrade, SerbiaDepartment of Pathology, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaDepartment of Ruminants and Swine Diseases, Faculty of Veterinary Medicine, University of Belgrade, SerbiaDepartment of Pathology, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaDepartment of Virology, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaDepartment of Virology, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaDepartment of Food Hygiene, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaDepartment of Immunology, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaUniversity Clinical Center of Serbia, Clinic for Digestive Surgery, Belgrade, SerbiaDepartment of Immunology, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaUniversity Clinical Center of Serbia, Clinic for Digestive Surgery, Belgrade, Serbia Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms in different geographical areas. Little is known about the mechanism of liver damage and type of local immune response by HEV in swine. Therefore, the aim of this study was to determine the morphological and immunophenotypic characteristics of hepatic lesions caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12 slaughtered 10 weeks old pigs which were RT-PCR positive for HEV RNA in rectal swab samples have been used. Livers were macroscopically examined and samples were taken for histopathological, immunohistochemical (CD3, CD79α and TGF-β1), semiquantitative, morphometric analysis, RT-nested-PCR, PCR and bacteriological analysis. Microscopically, mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically, portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly CD3+ T cells (234.80 ± 79.98). An intense TGF-β1 positive reaction was observed within the mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT-nested-PCR. HEV is noncytopathic, and this finding provides further evidence for an immune mediated pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte damage is clearly demonstrated. https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1813SwineLiverHEVMicroscopic changesImmunohistochemistry |
| spellingShingle | Branislav Kureljušić Sanja Aleksić-Kovačević Božidar Savić Radiša Prodanović Nemanja Jezdimirović Vesna Milićević Jelena Maksimović-Zorić Jasna Kureljušić Jadranka Žutić Đorđe Knežević Ljiljana Spalević Vladimir Kukolj Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus Veterinaria Italiana Swine Liver HEV Microscopic changes Immunohistochemistry |
| title | Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus |
| title_full | Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus |
| title_fullStr | Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus |
| title_full_unstemmed | Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus |
| title_short | Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus |
| title_sort | morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis e virus |
| topic | Swine Liver HEV Microscopic changes Immunohistochemistry |
| url | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1813 |
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