Alternative expression of TCRζ related genes in patients with chronic myeloid leukemia
<p>Abstract</p> <p>A previous study has demonstrated a significant decrease in the TCRζ gene expression level in chronic myeloid leukemia (CML); thus, we further investigated the expression of TCRζ-regulating factors, the distribution of the TCRζ 3' untranslated region (3'...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-12-01
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| Series: | Journal of Hematology & Oncology |
| Subjects: | |
| Online Access: | http://www.jhoonline.org/content/5/1/74 |
| _version_ | 1818120434800918528 |
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| author | Zha Xianfeng Yan Xiaojuan Shen Qi Zhang Yuping Wu Xiuli Chen Shaohua Li Bo Yang Lijian Geng Suxia Weng Jianyu Du Xin Li Yangqiu |
| author_facet | Zha Xianfeng Yan Xiaojuan Shen Qi Zhang Yuping Wu Xiuli Chen Shaohua Li Bo Yang Lijian Geng Suxia Weng Jianyu Du Xin Li Yangqiu |
| author_sort | Zha Xianfeng |
| collection | DOAJ |
| description | <p>Abstract</p> <p>A previous study has demonstrated a significant decrease in the TCRζ gene expression level in chronic myeloid leukemia (CML); thus, we further investigated the expression of TCRζ-regulating factors, the distribution of the TCRζ 3' untranslated region (3'-UTR) splice variants, and the expression level and correlation of the alternative splicing factor/splicing factor 2 (ASF/SF-2), FcεRIγ and ZAP-70 genes. TCRζ 3'-UTR splice variants were identified in peripheral blood mononuclear cells (PBMCs) from 14 healthy individuals, 40 patients with CML and 22 patients with CML in complete remission (CML-CR) by RT-PCR. The expression level of the TCRζ, FcεRIγ, ASF/SF-2 and ZAP-70 genes was analyzed by real-time quantitative PCR. While the expression of TCRζ gene in the CML group was significantly lower than that in the healthy individual and CML-CR groups, a significantly higher expression of the FceRIγ and ASF/SF-2 genes was found in the CML group. Two types of splicing forms were detected in all of the healthy individual CML-CR cases: wild type (WT) TCRζ 3'-UTR and alternatively splieced (AS) TCRζ 3'-UTR which have been alternatively splieced in the WT TCRζ 3'-UTR . However, 35% of the CML cases contained only the wild type TCRζ 3'-UTR isoform. Based on the TCRζ 3'-UTR isoform expression characteristic, we divided the patients with CML into two subgroups: the WT<sup>+</sup>AS<sup>-</sup> CML group, containing patients that express only the wild type TCRζ 3'-UTR, and the WT<sup>+</sup>AS<sup>+</sup> CML group, which contained patients that expressed two TCRζ 3'-UTR isoforms. A significantly different ASF/SF-2 and FcεRIγ gene expression pattern was found between the WT<sup>+</sup>AS<sup>-</sup> and WT<sup>+</sup>AS<sup>+</sup>CML groups. We concluded that defective TCRζ expression may be characterized in the WT<sup>+</sup>AS<sup>-</sup>and WT<sup>+</sup>AS<sup>+</sup>CML subgroups by the different gene expression pattern. The overexpression of ASF/SF2, which alternatively splices the TCRζ 3’-UTR, is thought to participate in feedback regulation. The characteristics of TCRζ 3'-UTR alternative splicing may be a novel immunological marker for the evaluation of the CML immune status.</p> |
| first_indexed | 2024-12-11T05:26:02Z |
| format | Article |
| id | doaj.art-e2c16afe6d8c43e28f86a3e396a4574f |
| institution | Directory Open Access Journal |
| issn | 1756-8722 |
| language | English |
| last_indexed | 2024-12-11T05:26:02Z |
| publishDate | 2012-12-01 |
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| series | Journal of Hematology & Oncology |
| spelling | doaj.art-e2c16afe6d8c43e28f86a3e396a4574f2022-12-22T01:19:34ZengBMCJournal of Hematology & Oncology1756-87222012-12-01517410.1186/1756-8722-5-74Alternative expression of TCRζ related genes in patients with chronic myeloid leukemiaZha XianfengYan XiaojuanShen QiZhang YupingWu XiuliChen ShaohuaLi BoYang LijianGeng SuxiaWeng JianyuDu XinLi Yangqiu<p>Abstract</p> <p>A previous study has demonstrated a significant decrease in the TCRζ gene expression level in chronic myeloid leukemia (CML); thus, we further investigated the expression of TCRζ-regulating factors, the distribution of the TCRζ 3' untranslated region (3'-UTR) splice variants, and the expression level and correlation of the alternative splicing factor/splicing factor 2 (ASF/SF-2), FcεRIγ and ZAP-70 genes. TCRζ 3'-UTR splice variants were identified in peripheral blood mononuclear cells (PBMCs) from 14 healthy individuals, 40 patients with CML and 22 patients with CML in complete remission (CML-CR) by RT-PCR. The expression level of the TCRζ, FcεRIγ, ASF/SF-2 and ZAP-70 genes was analyzed by real-time quantitative PCR. While the expression of TCRζ gene in the CML group was significantly lower than that in the healthy individual and CML-CR groups, a significantly higher expression of the FceRIγ and ASF/SF-2 genes was found in the CML group. Two types of splicing forms were detected in all of the healthy individual CML-CR cases: wild type (WT) TCRζ 3'-UTR and alternatively splieced (AS) TCRζ 3'-UTR which have been alternatively splieced in the WT TCRζ 3'-UTR . However, 35% of the CML cases contained only the wild type TCRζ 3'-UTR isoform. Based on the TCRζ 3'-UTR isoform expression characteristic, we divided the patients with CML into two subgroups: the WT<sup>+</sup>AS<sup>-</sup> CML group, containing patients that express only the wild type TCRζ 3'-UTR, and the WT<sup>+</sup>AS<sup>+</sup> CML group, which contained patients that expressed two TCRζ 3'-UTR isoforms. A significantly different ASF/SF-2 and FcεRIγ gene expression pattern was found between the WT<sup>+</sup>AS<sup>-</sup> and WT<sup>+</sup>AS<sup>+</sup>CML groups. We concluded that defective TCRζ expression may be characterized in the WT<sup>+</sup>AS<sup>-</sup>and WT<sup>+</sup>AS<sup>+</sup>CML subgroups by the different gene expression pattern. The overexpression of ASF/SF2, which alternatively splices the TCRζ 3’-UTR, is thought to participate in feedback regulation. The characteristics of TCRζ 3'-UTR alternative splicing may be a novel immunological marker for the evaluation of the CML immune status.</p>http://www.jhoonline.org/content/5/1/74ASF/SF-2geneTCRζ3<sup>′</sup>-UTRTCRζ geneFcεRIγ geneChronic myeloid leukemiaReal-time PCR |
| spellingShingle | Zha Xianfeng Yan Xiaojuan Shen Qi Zhang Yuping Wu Xiuli Chen Shaohua Li Bo Yang Lijian Geng Suxia Weng Jianyu Du Xin Li Yangqiu Alternative expression of TCRζ related genes in patients with chronic myeloid leukemia Journal of Hematology & Oncology ASF/SF-2gene TCRζ3<sup>′</sup>-UTR TCRζ gene FcεRIγ gene Chronic myeloid leukemia Real-time PCR |
| title | Alternative expression of TCRζ related genes in patients with chronic myeloid leukemia |
| title_full | Alternative expression of TCRζ related genes in patients with chronic myeloid leukemia |
| title_fullStr | Alternative expression of TCRζ related genes in patients with chronic myeloid leukemia |
| title_full_unstemmed | Alternative expression of TCRζ related genes in patients with chronic myeloid leukemia |
| title_short | Alternative expression of TCRζ related genes in patients with chronic myeloid leukemia |
| title_sort | alternative expression of tcrζ related genes in patients with chronic myeloid leukemia |
| topic | ASF/SF-2gene TCRζ3<sup>′</sup>-UTR TCRζ gene FcεRIγ gene Chronic myeloid leukemia Real-time PCR |
| url | http://www.jhoonline.org/content/5/1/74 |
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