Characterization of Codonopsis pilosula subsp. tangshen plastome and comparative analysis of Codonopsis species
Codonopsis pilosula subsp. tangshen is one of the most important medicinal herbs used in traditional Chinese medicine. Correct identification of materials from C. pilosula subsp. tangshen is critical to ensure the efficacy and safety of the associated medicines. Traditional DNA molecular markers cou...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342729/?tool=EBI |
| _version_ | 1811221436129345536 |
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| author | Jingwen Yue Yang Ni Mei Jiang Haimei Chen Pinghua Chen Chang Liu |
| author_facet | Jingwen Yue Yang Ni Mei Jiang Haimei Chen Pinghua Chen Chang Liu |
| author_sort | Jingwen Yue |
| collection | DOAJ |
| description | Codonopsis pilosula subsp. tangshen is one of the most important medicinal herbs used in traditional Chinese medicine. Correct identification of materials from C. pilosula subsp. tangshen is critical to ensure the efficacy and safety of the associated medicines. Traditional DNA molecular markers could distinguish Codonopsis species well, so we need to develop super or specific molecular markers. In this study, we reported the plastome of Codonopsis pilosula subsp. tangshen (Oliv.) D.Y. Hong conducted phylogenomic and comparative analyses in the Codonopsis genus for the first time. The entire length of the Codonopsis pilosula subsp. tangshen plastome was 170,672 bp. There were 108 genes in the plastome, including 76 protein-coding genes, 28 transfer RNA (tRNA), and four ribosomal RNA (rRNA) genes. Comparative analysis indicated that Codonopsis pilosula subsp. tangshen had an unusual large inversion in the large single-copy (LSC) region compared with the other three Codonopsis species. And there were two dispersed repeat sequences at both ends of the inverted regions, which might mediate the generation of this inversion. We found five hypervariable regions among the four Codonopsis species. PCR amplification and Sanger sequencing experiments demonstrated that two hypervariable regions could distinguish three medicinal Codonopsis species. Results obtained from this study will support taxonomic classification, discrimination, and molecular evolutionary studies of Codonopsis species. |
| first_indexed | 2024-04-12T07:59:18Z |
| format | Article |
| id | doaj.art-e2c3024e8c9d43eb81dcd26b8b7597cb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T07:59:18Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-e2c3024e8c9d43eb81dcd26b8b7597cb2022-12-22T03:41:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01178Characterization of Codonopsis pilosula subsp. tangshen plastome and comparative analysis of Codonopsis speciesJingwen YueYang NiMei JiangHaimei ChenPinghua ChenChang LiuCodonopsis pilosula subsp. tangshen is one of the most important medicinal herbs used in traditional Chinese medicine. Correct identification of materials from C. pilosula subsp. tangshen is critical to ensure the efficacy and safety of the associated medicines. Traditional DNA molecular markers could distinguish Codonopsis species well, so we need to develop super or specific molecular markers. In this study, we reported the plastome of Codonopsis pilosula subsp. tangshen (Oliv.) D.Y. Hong conducted phylogenomic and comparative analyses in the Codonopsis genus for the first time. The entire length of the Codonopsis pilosula subsp. tangshen plastome was 170,672 bp. There were 108 genes in the plastome, including 76 protein-coding genes, 28 transfer RNA (tRNA), and four ribosomal RNA (rRNA) genes. Comparative analysis indicated that Codonopsis pilosula subsp. tangshen had an unusual large inversion in the large single-copy (LSC) region compared with the other three Codonopsis species. And there were two dispersed repeat sequences at both ends of the inverted regions, which might mediate the generation of this inversion. We found five hypervariable regions among the four Codonopsis species. PCR amplification and Sanger sequencing experiments demonstrated that two hypervariable regions could distinguish three medicinal Codonopsis species. Results obtained from this study will support taxonomic classification, discrimination, and molecular evolutionary studies of Codonopsis species.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342729/?tool=EBI |
| spellingShingle | Jingwen Yue Yang Ni Mei Jiang Haimei Chen Pinghua Chen Chang Liu Characterization of Codonopsis pilosula subsp. tangshen plastome and comparative analysis of Codonopsis species PLoS ONE |
| title | Characterization of Codonopsis pilosula subsp. tangshen plastome and comparative analysis of Codonopsis species |
| title_full | Characterization of Codonopsis pilosula subsp. tangshen plastome and comparative analysis of Codonopsis species |
| title_fullStr | Characterization of Codonopsis pilosula subsp. tangshen plastome and comparative analysis of Codonopsis species |
| title_full_unstemmed | Characterization of Codonopsis pilosula subsp. tangshen plastome and comparative analysis of Codonopsis species |
| title_short | Characterization of Codonopsis pilosula subsp. tangshen plastome and comparative analysis of Codonopsis species |
| title_sort | characterization of codonopsis pilosula subsp tangshen plastome and comparative analysis of codonopsis species |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342729/?tool=EBI |
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