Study on the stability of internal quality control sera for HIV/AIDS immunodiagnostic tests
Introduction: The use of reference materials in order to assure and perform the quality control of analytical measurements is a requirement in clinical laboratories. Objectives: Stability of serum samples, kept frozen at -20°C for long-term storage and at varied temperatures during short period...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Patologia Clínica
2014-02-01
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| Series: | Jornal Brasileiro de Patologia e Medicina Laboratorial |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000100036&lng=en&tlng=en |
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| author | Márcia Jorge Castejon Rosemeire Yamashiro Camila Cardoso de Oliveira Daniel Granato Carmem Aparecida de Freitas Oliveira Mirthes Ueda |
| author_facet | Márcia Jorge Castejon Rosemeire Yamashiro Camila Cardoso de Oliveira Daniel Granato Carmem Aparecida de Freitas Oliveira Mirthes Ueda |
| author_sort | Márcia Jorge Castejon |
| collection | DOAJ |
| description | Introduction: The use of reference materials in order to assure and perform the quality control of analytical measurements is a requirement in clinical laboratories. Objectives: Stability of serum samples, kept frozen at -20°C for long-term storage and at varied temperatures during short periods, was evaluated by investigating the persistency of anti-human immunodeficiency virus (HIV) antibodies reactivity on enzyme-linked immunosorbent assay/enzyme immunoassay (ELISA/EIA), Western blot and indirect immunofluorescence assays. Method: The analyzed sera were part of serum panels (comprised of anti-HIV positive and negative samples), produced at the Immunology Center of Instituto Adolfo Lutz, which have been the reference specimens for producing the internal quality assurance sera of HIV/acquired immunodeficiency syndrome (AIDS) immunodiagnostic assays. Sera stability was assessed in samples stored at -20°C for 56 weeks, and at various temperature conditions: from 2°C to 8°C (refrigerator), from 15°C to 25°C (room temperature), at 37°C (incubator) and at -80°C (freezer) for 24 and 48 hours. The statistical analyses on HIV-negative serum samples (long-term storage) were significant (p < 0.05), and neither adverse effects on these samples as the occurrence of false-positive results nor false-negative results in HIV antibody positive sera were found in both studies. Conclusion: It was possible to conclude that the reference material remained stable for 48 hours at different temperatures (short-term) and it remained stable at -20°C for 56 weeks (long-term). |
| first_indexed | 2024-04-11T22:47:36Z |
| format | Article |
| id | doaj.art-e2c42937c24f48638165c67bf164ded7 |
| institution | Directory Open Access Journal |
| issn | 1678-4774 |
| language | English |
| last_indexed | 2024-04-11T22:47:36Z |
| publishDate | 2014-02-01 |
| publisher | Sociedade Brasileira de Patologia Clínica |
| record_format | Article |
| series | Jornal Brasileiro de Patologia e Medicina Laboratorial |
| spelling | doaj.art-e2c42937c24f48638165c67bf164ded72022-12-22T03:58:42ZengSociedade Brasileira de Patologia ClínicaJornal Brasileiro de Patologia e Medicina Laboratorial1678-47742014-02-01501364510.1590/S1676-24442014000100005S1676-24442014000100036Study on the stability of internal quality control sera for HIV/AIDS immunodiagnostic testsMárcia Jorge CastejonRosemeire YamashiroCamila Cardoso de OliveiraDaniel GranatoCarmem Aparecida de Freitas OliveiraMirthes UedaIntroduction: The use of reference materials in order to assure and perform the quality control of analytical measurements is a requirement in clinical laboratories. Objectives: Stability of serum samples, kept frozen at -20°C for long-term storage and at varied temperatures during short periods, was evaluated by investigating the persistency of anti-human immunodeficiency virus (HIV) antibodies reactivity on enzyme-linked immunosorbent assay/enzyme immunoassay (ELISA/EIA), Western blot and indirect immunofluorescence assays. Method: The analyzed sera were part of serum panels (comprised of anti-HIV positive and negative samples), produced at the Immunology Center of Instituto Adolfo Lutz, which have been the reference specimens for producing the internal quality assurance sera of HIV/acquired immunodeficiency syndrome (AIDS) immunodiagnostic assays. Sera stability was assessed in samples stored at -20°C for 56 weeks, and at various temperature conditions: from 2°C to 8°C (refrigerator), from 15°C to 25°C (room temperature), at 37°C (incubator) and at -80°C (freezer) for 24 and 48 hours. The statistical analyses on HIV-negative serum samples (long-term storage) were significant (p < 0.05), and neither adverse effects on these samples as the occurrence of false-positive results nor false-negative results in HIV antibody positive sera were found in both studies. Conclusion: It was possible to conclude that the reference material remained stable for 48 hours at different temperatures (short-term) and it remained stable at -20°C for 56 weeks (long-term).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000100036&lng=en&tlng=enestabilidadesoro controleanticorpos anti-HIVtemperaturacontrole de qualidade |
| spellingShingle | Márcia Jorge Castejon Rosemeire Yamashiro Camila Cardoso de Oliveira Daniel Granato Carmem Aparecida de Freitas Oliveira Mirthes Ueda Study on the stability of internal quality control sera for HIV/AIDS immunodiagnostic tests Jornal Brasileiro de Patologia e Medicina Laboratorial estabilidade soro controle anticorpos anti-HIV temperatura controle de qualidade |
| title | Study on the stability of internal quality control sera for HIV/AIDS immunodiagnostic tests |
| title_full | Study on the stability of internal quality control sera for HIV/AIDS immunodiagnostic tests |
| title_fullStr | Study on the stability of internal quality control sera for HIV/AIDS immunodiagnostic tests |
| title_full_unstemmed | Study on the stability of internal quality control sera for HIV/AIDS immunodiagnostic tests |
| title_short | Study on the stability of internal quality control sera for HIV/AIDS immunodiagnostic tests |
| title_sort | study on the stability of internal quality control sera for hiv aids immunodiagnostic tests |
| topic | estabilidade soro controle anticorpos anti-HIV temperatura controle de qualidade |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000100036&lng=en&tlng=en |
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