Age group DNA methylation differences in lemon sharks (Negaprion brevirostris): Implications for future age estimation tools
Abstract Age information is often non‐existent for most shark populations due to a lack of measurable physiological and morphological traits that can be used to estimate age. Recently, epigenetic clocks have been found to accurately estimate age for mammals, birds, and fish. However, since these clo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-08-01
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| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.9226 |
| _version_ | 1798016372904558592 |
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| author | Andria Paige Beal Serena Hackerott Kevin Feldheim Samuel H. Gruber Jose M. Eirin‐Lopez |
| author_facet | Andria Paige Beal Serena Hackerott Kevin Feldheim Samuel H. Gruber Jose M. Eirin‐Lopez |
| author_sort | Andria Paige Beal |
| collection | DOAJ |
| description | Abstract Age information is often non‐existent for most shark populations due to a lack of measurable physiological and morphological traits that can be used to estimate age. Recently, epigenetic clocks have been found to accurately estimate age for mammals, birds, and fish. However, since these clocks rely, among other things, on the availability of reference genomes, their application is hampered in non‐traditional model organisms lacking such molecular resources. The technique known as Methyl‐Sensitive Amplified Polymorphism (MSAP) has emerged as a valid alternative for studying DNA methylation biomarkers when reference genome information is missing, and large numbers of samples need to be processed. Accordingly, the MSAP technique was used in the present study to characterize global DNA methylation patterns in lemon sharks from three different age groups (juveniles, subadults, and adults). The obtained results reveal that, while MSAP analyses lack enough resolution as a standalone approach to infer age in these organisms, the global DNA methylation patterns observed using this technique displayed significant differences between age groups. Overall, these results confer that DNA methylation does change with age in sharks like what has been seen for other vertebrates and that MSAP could be useful as part of an epigenetics pipeline to infer the broad range of ages found in large samples sizes. |
| first_indexed | 2024-04-11T15:49:39Z |
| format | Article |
| id | doaj.art-e2c9e0d67f574ac29080281ba36fc158 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-04-11T15:49:39Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-e2c9e0d67f574ac29080281ba36fc1582022-12-22T04:15:26ZengWileyEcology and Evolution2045-77582022-08-01128n/an/a10.1002/ece3.9226Age group DNA methylation differences in lemon sharks (Negaprion brevirostris): Implications for future age estimation toolsAndria Paige Beal0Serena Hackerott1Kevin Feldheim2Samuel H. Gruber3Jose M. Eirin‐Lopez4Environmental Epigenetics Laboratory, Institute of Environment Florida International University Miami Florida USAEnvironmental Epigenetics Laboratory, Institute of Environment Florida International University Miami Florida USAPritzker Laboratory for Molecular Systematics and Evolution Field Museum of Natural History Chicago Illinois USABimini Biological Field Station Foundation South Bimini BahamasEnvironmental Epigenetics Laboratory, Institute of Environment Florida International University Miami Florida USAAbstract Age information is often non‐existent for most shark populations due to a lack of measurable physiological and morphological traits that can be used to estimate age. Recently, epigenetic clocks have been found to accurately estimate age for mammals, birds, and fish. However, since these clocks rely, among other things, on the availability of reference genomes, their application is hampered in non‐traditional model organisms lacking such molecular resources. The technique known as Methyl‐Sensitive Amplified Polymorphism (MSAP) has emerged as a valid alternative for studying DNA methylation biomarkers when reference genome information is missing, and large numbers of samples need to be processed. Accordingly, the MSAP technique was used in the present study to characterize global DNA methylation patterns in lemon sharks from three different age groups (juveniles, subadults, and adults). The obtained results reveal that, while MSAP analyses lack enough resolution as a standalone approach to infer age in these organisms, the global DNA methylation patterns observed using this technique displayed significant differences between age groups. Overall, these results confer that DNA methylation does change with age in sharks like what has been seen for other vertebrates and that MSAP could be useful as part of an epigenetics pipeline to infer the broad range of ages found in large samples sizes.https://doi.org/10.1002/ece3.9226age estimationconservationDNA methylationMSAPsharks |
| spellingShingle | Andria Paige Beal Serena Hackerott Kevin Feldheim Samuel H. Gruber Jose M. Eirin‐Lopez Age group DNA methylation differences in lemon sharks (Negaprion brevirostris): Implications for future age estimation tools Ecology and Evolution age estimation conservation DNA methylation MSAP sharks |
| title | Age group DNA methylation differences in lemon sharks (Negaprion brevirostris): Implications for future age estimation tools |
| title_full | Age group DNA methylation differences in lemon sharks (Negaprion brevirostris): Implications for future age estimation tools |
| title_fullStr | Age group DNA methylation differences in lemon sharks (Negaprion brevirostris): Implications for future age estimation tools |
| title_full_unstemmed | Age group DNA methylation differences in lemon sharks (Negaprion brevirostris): Implications for future age estimation tools |
| title_short | Age group DNA methylation differences in lemon sharks (Negaprion brevirostris): Implications for future age estimation tools |
| title_sort | age group dna methylation differences in lemon sharks negaprion brevirostris implications for future age estimation tools |
| topic | age estimation conservation DNA methylation MSAP sharks |
| url | https://doi.org/10.1002/ece3.9226 |
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