Characterization of a New Chitosanase from a Marine <i>Bacillus</i> sp. and the Anti-Oxidant Activity of Its Hydrolysate
Chitooligosaccharide (COS) has been recognized to exhibit efficient anti-oxidant activity. Enzymatic hydrolysis using chitosanases can retain all the amino and hydroxyl groups of chitosan, which are necessary for its activity. In this study, a new chitosanase encoding gene, <i>csnQ</i>,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/2/126 |
| _version_ | 1798003865881149440 |
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| author | Chunrui Ma Xiao Li Kun Yang Shangyong Li |
| author_facet | Chunrui Ma Xiao Li Kun Yang Shangyong Li |
| author_sort | Chunrui Ma |
| collection | DOAJ |
| description | Chitooligosaccharide (COS) has been recognized to exhibit efficient anti-oxidant activity. Enzymatic hydrolysis using chitosanases can retain all the amino and hydroxyl groups of chitosan, which are necessary for its activity. In this study, a new chitosanase encoding gene, <i>csnQ</i>, was cloned from the marine <i>Bacillus</i> sp. Q1098 and expressed in <i>Escherichia coli</i>. The recombinant chitosanase, CsnQ, showed maximal activity at pH 5.31 and 60 °C. Determination of CsnQ pH-stability showed that CsnQ could retain more than 50% of its activity over a wide pH, from 3.60 to 9.80. CsnQ is an endo-type chitosanase, yielding chitodisaccharide as the main product. Additionally, in vitro and in vivo analyses indicated that chitodisaccharide possesses much more effective anti-oxidant activity than glucosamine and low molecular weight chitosan (LMW-CS) (~5 kDa). Notably, to our knowledge, this is the first evidence that chitodisaccharide is the minimal COS fragment required for free radical scavenging. |
| first_indexed | 2024-04-11T12:14:24Z |
| format | Article |
| id | doaj.art-e2ca0c3ce4674deeb62411b47beae582 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T12:14:24Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e2ca0c3ce4674deeb62411b47beae5822022-12-22T04:24:27ZengMDPI AGMarine Drugs1660-33972020-02-0118212610.3390/md18020126md18020126Characterization of a New Chitosanase from a Marine <i>Bacillus</i> sp. and the Anti-Oxidant Activity of Its HydrolysateChunrui Ma0Xiao Li1Kun Yang2Shangyong Li3College of Basic Medicine, Qingdao University, Qingdao 266071, ChinaCollege of Basic Medicine, Qingdao University, Qingdao 266071, ChinaCollege of Basic Medicine, Qingdao University, Qingdao 266071, ChinaCollege of Basic Medicine, Qingdao University, Qingdao 266071, ChinaChitooligosaccharide (COS) has been recognized to exhibit efficient anti-oxidant activity. Enzymatic hydrolysis using chitosanases can retain all the amino and hydroxyl groups of chitosan, which are necessary for its activity. In this study, a new chitosanase encoding gene, <i>csnQ</i>, was cloned from the marine <i>Bacillus</i> sp. Q1098 and expressed in <i>Escherichia coli</i>. The recombinant chitosanase, CsnQ, showed maximal activity at pH 5.31 and 60 °C. Determination of CsnQ pH-stability showed that CsnQ could retain more than 50% of its activity over a wide pH, from 3.60 to 9.80. CsnQ is an endo-type chitosanase, yielding chitodisaccharide as the main product. Additionally, in vitro and in vivo analyses indicated that chitodisaccharide possesses much more effective anti-oxidant activity than glucosamine and low molecular weight chitosan (LMW-CS) (~5 kDa). Notably, to our knowledge, this is the first evidence that chitodisaccharide is the minimal COS fragment required for free radical scavenging.https://www.mdpi.com/1660-3397/18/2/126chitosanaseph-stability<i>bacillus</i> sp. q1098chitooligosaccharideanti-oxidant activity |
| spellingShingle | Chunrui Ma Xiao Li Kun Yang Shangyong Li Characterization of a New Chitosanase from a Marine <i>Bacillus</i> sp. and the Anti-Oxidant Activity of Its Hydrolysate Marine Drugs chitosanase ph-stability <i>bacillus</i> sp. q1098 chitooligosaccharide anti-oxidant activity |
| title | Characterization of a New Chitosanase from a Marine <i>Bacillus</i> sp. and the Anti-Oxidant Activity of Its Hydrolysate |
| title_full | Characterization of a New Chitosanase from a Marine <i>Bacillus</i> sp. and the Anti-Oxidant Activity of Its Hydrolysate |
| title_fullStr | Characterization of a New Chitosanase from a Marine <i>Bacillus</i> sp. and the Anti-Oxidant Activity of Its Hydrolysate |
| title_full_unstemmed | Characterization of a New Chitosanase from a Marine <i>Bacillus</i> sp. and the Anti-Oxidant Activity of Its Hydrolysate |
| title_short | Characterization of a New Chitosanase from a Marine <i>Bacillus</i> sp. and the Anti-Oxidant Activity of Its Hydrolysate |
| title_sort | characterization of a new chitosanase from a marine i bacillus i sp and the anti oxidant activity of its hydrolysate |
| topic | chitosanase ph-stability <i>bacillus</i> sp. q1098 chitooligosaccharide anti-oxidant activity |
| url | https://www.mdpi.com/1660-3397/18/2/126 |
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