Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A
Abstract Background Senecavirus A (SVA) is a pathogen that has recently caused porcine idiopathic vesicular disease (PIVD). The clinical signs are similar to those of foot-and-mouth disease, porcine vesicular disease, and vesicular stomatitis. Therefore, identification of SVA as a cause of PIVD is i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-022-01934-8 |
| _version_ | 1811190502495617024 |
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| author | Zhongyuan Ma Jianliang Lv Zhongwang Zhang Li Pan |
| author_facet | Zhongyuan Ma Jianliang Lv Zhongwang Zhang Li Pan |
| author_sort | Zhongyuan Ma |
| collection | DOAJ |
| description | Abstract Background Senecavirus A (SVA) is a pathogen that has recently caused porcine idiopathic vesicular disease (PIVD). The clinical signs are similar to those of foot-and-mouth disease, porcine vesicular disease, and vesicular stomatitis. Therefore, identification of SVA as a cause of PIVD is important to eliminate this emerging pathogen. Methods In this study, an indirect ELISA based on the VP2 epitope (VP2-epitp-ELISA) was developed to detect antibodies directed against SVA. Results A novel linear epitope (271GLRNRFTTGTDEEQ284) was first identified at the C-terminus of the VP2 protein by epitope mapping. The diagnostic performance of VP2-epitp-ELISA was estimated by testing a panel of known background sera from swine. Under the optimum test conditions, when the cutoff value was 37%, the diagnostic sensitivity (Dn) and diagnostic specificity (Dp) of the assay were 91.13% and 91.17%, respectively. The accuracy of VP2-epitp-ELISA was validated and further compared with that of commercial diagnostic kits. The diagnostic results showed that VP2-epitp-ELISA did not cross-react with serum positive for other idiopathic vesicular diseases and had a concordance rate of 90.41% with the Swinecheck® SVA bELISA. Conclusions These results indicate that VP2-epitp-ELISA is suitable for specific detection of antibodies against SVA in swine. |
| first_indexed | 2024-04-11T14:52:15Z |
| format | Article |
| id | doaj.art-e2cc3ec23a1f405680523481e8b8a673 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-11T14:52:15Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-e2cc3ec23a1f405680523481e8b8a6732022-12-22T04:17:25ZengBMCVirology Journal1743-422X2022-12-011911710.1186/s12985-022-01934-8Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus AZhongyuan Ma0Jianliang Lv1Zhongwang Zhang2Li Pan3State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou Veterinary Research InstituteState Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou Veterinary Research InstituteState Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou Veterinary Research InstituteState Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou Veterinary Research InstituteAbstract Background Senecavirus A (SVA) is a pathogen that has recently caused porcine idiopathic vesicular disease (PIVD). The clinical signs are similar to those of foot-and-mouth disease, porcine vesicular disease, and vesicular stomatitis. Therefore, identification of SVA as a cause of PIVD is important to eliminate this emerging pathogen. Methods In this study, an indirect ELISA based on the VP2 epitope (VP2-epitp-ELISA) was developed to detect antibodies directed against SVA. Results A novel linear epitope (271GLRNRFTTGTDEEQ284) was first identified at the C-terminus of the VP2 protein by epitope mapping. The diagnostic performance of VP2-epitp-ELISA was estimated by testing a panel of known background sera from swine. Under the optimum test conditions, when the cutoff value was 37%, the diagnostic sensitivity (Dn) and diagnostic specificity (Dp) of the assay were 91.13% and 91.17%, respectively. The accuracy of VP2-epitp-ELISA was validated and further compared with that of commercial diagnostic kits. The diagnostic results showed that VP2-epitp-ELISA did not cross-react with serum positive for other idiopathic vesicular diseases and had a concordance rate of 90.41% with the Swinecheck® SVA bELISA. Conclusions These results indicate that VP2-epitp-ELISA is suitable for specific detection of antibodies against SVA in swine.https://doi.org/10.1186/s12985-022-01934-8DiagnosisSenecavirus AVP2EpitopeELISA |
| spellingShingle | Zhongyuan Ma Jianliang Lv Zhongwang Zhang Li Pan Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A Virology Journal Diagnosis Senecavirus A VP2 Epitope ELISA |
| title | Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A |
| title_full | Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A |
| title_fullStr | Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A |
| title_full_unstemmed | Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A |
| title_short | Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A |
| title_sort | development of an indirect elisa using a novel linear epitope at the c terminal region of the vp2 protein to specifically detect antibodies against senecavirus a |
| topic | Diagnosis Senecavirus A VP2 Epitope ELISA |
| url | https://doi.org/10.1186/s12985-022-01934-8 |
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