The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy
The search for effective neuroprotective agents for the treatment of neurotrauma has always been of great interest to researchers around the world. Extracellular heat shock protein 70 (eHsp70) is considered a promising agent to study, as it has been demonstrated to exert a significant neuroprotectiv...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/1/93 |
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| author | Svetlana V. Demyanenko Maria A. Pitinova Valentina A. Dzreyan Yuliya N. Kalyuzhnaya Moez A. Eid Andrey Y. Abramov Michael B. Evgen’ev David G. Garbuz |
| author_facet | Svetlana V. Demyanenko Maria A. Pitinova Valentina A. Dzreyan Yuliya N. Kalyuzhnaya Moez A. Eid Andrey Y. Abramov Michael B. Evgen’ev David G. Garbuz |
| author_sort | Svetlana V. Demyanenko |
| collection | DOAJ |
| description | The search for effective neuroprotective agents for the treatment of neurotrauma has always been of great interest to researchers around the world. Extracellular heat shock protein 70 (eHsp70) is considered a promising agent to study, as it has been demonstrated to exert a significant neuroprotective activity against various neurodegenerative diseases. We showed that eHsp70 can penetrate neurons and glial cells when added to the incubation medium, and can accumulate in the nuclei of neurons and satellite glial cells after axotomy. eHsp70 reduces apoptosis and necrosis of the glial cells, but not the neurons. At the same time, co-localization of eHsp70 with p53 protein, one of the key regulators of apoptosis, was noted. eHsp70 reduces the level of the p53 protein apoptosis promoter both in glial cells and in the nuclei and cytoplasm of neurons, which indicates its neuroprotective effect. The ability of eHsp70 to reverse the proapoptotic effect of the p53 activator WR1065 may indicate its ability to regulate p53 activity or its proteosome-dependent degradation. |
| first_indexed | 2024-03-10T03:46:51Z |
| format | Article |
| id | doaj.art-e2cd49df09f44470a661b15eb7fcadc1 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T03:46:51Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-e2cd49df09f44470a661b15eb7fcadc12023-11-23T11:20:05ZengMDPI AGCells2073-44092021-12-011119310.3390/cells11010093The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during AxotomySvetlana V. Demyanenko0Maria A. Pitinova1Valentina A. Dzreyan2Yuliya N. Kalyuzhnaya3Moez A. Eid4Andrey Y. Abramov5Michael B. Evgen’ev6David G. Garbuz7Laboratory of Molecular Neurobiology, Academy of Biology and Biotechnology, Southern Federal University, 344090 Rostov-on-Don, RussiaLaboratory of Molecular Neurobiology, Academy of Biology and Biotechnology, Southern Federal University, 344090 Rostov-on-Don, RussiaLaboratory of Molecular Neurobiology, Academy of Biology and Biotechnology, Southern Federal University, 344090 Rostov-on-Don, RussiaLaboratory of Molecular Neurobiology, Academy of Biology and Biotechnology, Southern Federal University, 344090 Rostov-on-Don, RussiaLaboratory of Molecular Neurobiology, Academy of Biology and Biotechnology, Southern Federal University, 344090 Rostov-on-Don, RussiaDepartment of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, Queen Square, London WC1N 3BG, UKEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaThe search for effective neuroprotective agents for the treatment of neurotrauma has always been of great interest to researchers around the world. Extracellular heat shock protein 70 (eHsp70) is considered a promising agent to study, as it has been demonstrated to exert a significant neuroprotective activity against various neurodegenerative diseases. We showed that eHsp70 can penetrate neurons and glial cells when added to the incubation medium, and can accumulate in the nuclei of neurons and satellite glial cells after axotomy. eHsp70 reduces apoptosis and necrosis of the glial cells, but not the neurons. At the same time, co-localization of eHsp70 with p53 protein, one of the key regulators of apoptosis, was noted. eHsp70 reduces the level of the p53 protein apoptosis promoter both in glial cells and in the nuclei and cytoplasm of neurons, which indicates its neuroprotective effect. The ability of eHsp70 to reverse the proapoptotic effect of the p53 activator WR1065 may indicate its ability to regulate p53 activity or its proteosome-dependent degradation.https://www.mdpi.com/2073-4409/11/1/93neurotraumaaxotomyexogenous Hsp70p53 |
| spellingShingle | Svetlana V. Demyanenko Maria A. Pitinova Valentina A. Dzreyan Yuliya N. Kalyuzhnaya Moez A. Eid Andrey Y. Abramov Michael B. Evgen’ev David G. Garbuz The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy Cells neurotrauma axotomy exogenous Hsp70 p53 |
| title | The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy |
| title_full | The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy |
| title_fullStr | The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy |
| title_full_unstemmed | The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy |
| title_short | The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy |
| title_sort | role of p53 protein in the realization of the exogenous heat shock protein 70 anti apoptotic effect during axotomy |
| topic | neurotrauma axotomy exogenous Hsp70 p53 |
| url | https://www.mdpi.com/2073-4409/11/1/93 |
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