Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from <i>Brassica napus</i> L. Based on RNA-Seq and Metabolome
<i>Brassica napus</i> L. is a vital plant oil resource worldwide. The fatty acid biosynthesis and oil accumulation in its seeds are controlled by several genetic and environmental factors, including daytime and nighttime temperatures. We analyzed changes in oleic and erucic acid content...
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MDPI AG
2023-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/2/325 |
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| author | Chao Mi Chao Sun Yuting Yuan Fei Li Qian Wang Haiping Zhu Shuijin Hua Liangbin Lin |
| author_facet | Chao Mi Chao Sun Yuting Yuan Fei Li Qian Wang Haiping Zhu Shuijin Hua Liangbin Lin |
| author_sort | Chao Mi |
| collection | DOAJ |
| description | <i>Brassica napus</i> L. is a vital plant oil resource worldwide. The fatty acid biosynthesis and oil accumulation in its seeds are controlled by several genetic and environmental factors, including daytime and nighttime temperatures. We analyzed changes in oleic and erucic acid content in two double haploid (DH) lines, DH0729, a weakly temperature-sensitive line, and DH0815, a strongly temperature-sensitive line, derived from <i>B. napus</i> plants grown at different altitudes (1600, 1800, 2000, 2200, and 2400 m a.s.l., 28.85° N, 112.35° E) and nighttime temperatures (20/18, 20/16, 20/13 and 20/10 °C, daytime/nighttime temperature). Based on medium- and long-chain fatty acid metabolites, the total oleic acid content 35 and 43 days after flowering was significantly lower in low nighttime temperature (LNT, 20/13 °C) plants than in high nighttime temperature (HNT, 20/18 °C) plants (HNT: 58–62%; LNT: 49–54%; an average decrease of 9%), and the total erucic acid content was significantly lower in HNT than in LNT plants (HNT: 1–2%; LNT: 8–13%; an average increase of 10%). An RNA-seq analysis showed that the expression levels of <i>SAD</i> (LOC106366808), <i>ECR</i> (LOC106396280), <i>KCS</i> (LOC106419344), <i>KAR</i> (LOC106367337), <i>HB1</i>(LOC106430193), and <i>DOF5</i> (LOC111211868) in STSL seeds increased under LNT conditions. In STSL seeds, a base mutation in the <i>cis</i>-acting element involved in low-temperature responsiveness (LTR), the <i>HB1</i> and <i>KCS</i> promoter caused loss of sensitivity to low temperatures, whereas that of the <i>KCS</i> promoter caused increased sensitivity to low temperatures. |
| first_indexed | 2024-03-09T11:26:00Z |
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| id | doaj.art-e2cd8a0d0282406ab749c84e172e1849 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T11:26:00Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-e2cd8a0d0282406ab749c84e172e18492023-12-01T00:04:53ZengMDPI AGPlants2223-77472023-01-0112232510.3390/plants12020325Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from <i>Brassica napus</i> L. Based on RNA-Seq and MetabolomeChao Mi0Chao Sun1Yuting Yuan2Fei Li3Qian Wang4Haiping Zhu5Shuijin Hua6Liangbin Lin7College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaAgricultural Research Institute, Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa 850032, ChinaYunnan Key Laboratory for Wild Plant Resources, Department of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, ChinaHorticultural Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650200, ChinaFood Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650200, ChinaInstitute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, 17, Hangzhou 310021, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China<i>Brassica napus</i> L. is a vital plant oil resource worldwide. The fatty acid biosynthesis and oil accumulation in its seeds are controlled by several genetic and environmental factors, including daytime and nighttime temperatures. We analyzed changes in oleic and erucic acid content in two double haploid (DH) lines, DH0729, a weakly temperature-sensitive line, and DH0815, a strongly temperature-sensitive line, derived from <i>B. napus</i> plants grown at different altitudes (1600, 1800, 2000, 2200, and 2400 m a.s.l., 28.85° N, 112.35° E) and nighttime temperatures (20/18, 20/16, 20/13 and 20/10 °C, daytime/nighttime temperature). Based on medium- and long-chain fatty acid metabolites, the total oleic acid content 35 and 43 days after flowering was significantly lower in low nighttime temperature (LNT, 20/13 °C) plants than in high nighttime temperature (HNT, 20/18 °C) plants (HNT: 58–62%; LNT: 49–54%; an average decrease of 9%), and the total erucic acid content was significantly lower in HNT than in LNT plants (HNT: 1–2%; LNT: 8–13%; an average increase of 10%). An RNA-seq analysis showed that the expression levels of <i>SAD</i> (LOC106366808), <i>ECR</i> (LOC106396280), <i>KCS</i> (LOC106419344), <i>KAR</i> (LOC106367337), <i>HB1</i>(LOC106430193), and <i>DOF5</i> (LOC111211868) in STSL seeds increased under LNT conditions. In STSL seeds, a base mutation in the <i>cis</i>-acting element involved in low-temperature responsiveness (LTR), the <i>HB1</i> and <i>KCS</i> promoter caused loss of sensitivity to low temperatures, whereas that of the <i>KCS</i> promoter caused increased sensitivity to low temperatures.https://www.mdpi.com/2223-7747/12/2/325<i>Brassica napus</i> L.seedsnighttime temperaturefatty acid contentRNA-seqmetabolome |
| spellingShingle | Chao Mi Chao Sun Yuting Yuan Fei Li Qian Wang Haiping Zhu Shuijin Hua Liangbin Lin Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from <i>Brassica napus</i> L. Based on RNA-Seq and Metabolome Plants <i>Brassica napus</i> L. seeds nighttime temperature fatty acid content RNA-seq metabolome |
| title | Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from <i>Brassica napus</i> L. Based on RNA-Seq and Metabolome |
| title_full | Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from <i>Brassica napus</i> L. Based on RNA-Seq and Metabolome |
| title_fullStr | Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from <i>Brassica napus</i> L. Based on RNA-Seq and Metabolome |
| title_full_unstemmed | Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from <i>Brassica napus</i> L. Based on RNA-Seq and Metabolome |
| title_short | Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from <i>Brassica napus</i> L. Based on RNA-Seq and Metabolome |
| title_sort | effects of low nighttime temperature on fatty acid content in developing seeds from i brassica napus i l based on rna seq and metabolome |
| topic | <i>Brassica napus</i> L. seeds nighttime temperature fatty acid content RNA-seq metabolome |
| url | https://www.mdpi.com/2223-7747/12/2/325 |
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