Analgesic and anti-inflammatory activity of Artemisia extracts on animal models of nociception
Introduction. The study aims to assess the antinociceptive and anti-inflammatory activity of extracts obtained from indigenous species of wormwood: Artemisia absinthium, A. annua, A. vulgaris and A. pontica. Materials and methods. For these experiments, we employed nociception models using thermal...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Romanian Association of Balneology, Editura Balneara
2021-03-01
|
| Series: | Balneo and PRM Research Journal |
| Subjects: | |
| Online Access: | http://bioclima.ro/Balneo416.pdf |
| _version_ | 1818880955940601856 |
|---|---|
| author | IVANESCU Bianca CORCIOVA Andreia VLASE Laurian GHELDIU Ana-Maria MIRON Anca ABABEI Daniela Carmen BILD Veronica |
| author_facet | IVANESCU Bianca CORCIOVA Andreia VLASE Laurian GHELDIU Ana-Maria MIRON Anca ABABEI Daniela Carmen BILD Veronica |
| author_sort | IVANESCU Bianca |
| collection | DOAJ |
| description | Introduction. The study aims to assess the antinociceptive and anti-inflammatory activity of extracts obtained from indigenous species of wormwood: Artemisia absinthium, A. annua, A. vulgaris and A. pontica.
Materials and methods. For these experiments, we employed nociception models using thermal stimulus (hot plate and tail immersion tests, t = 52.5 0C; 30, 60 and 90 minutes testing), chemical stimulus (Zymosan-induced abdominal constriction response test, using distinct lots and testing at 60, 90 and 120 minutes after administration of samples) and pressure stimulus (Randall Selitto test) and an inflammation model for the evaluation of inflammatory edema by Plethysmometer test. Groups of 6 Swiss mice / lot were used, receiving by oral administration the plant extracts suspended in 0.1% CMC-Na. The doses were administered in geometric progression. Chemical analyses were performed by HPLC-MS in order to identify bioactive substances present in extracts: methoxylated flavonoids, sesquiterpene lactones, phytosterols and hydroxycinnamic acids.
Results and discussion. All plant extracts showed antinociceptive action on the models with thermal stimulus, as demonstrated by the ED50 values obtained at different test times. In the models of nociception with chemical and mechanical stimulus, models based on inflammatory mediation, the studied fractions have partially proved their antinociceptive action. Regarding the degree of inhibition of inflammatory edema, the highest potency was exhibited by Artemisia pontica extract (86.5% inhibition for the dose of 100 mg/kg). Hispidulin and eupatorin, known anti-inflammatory compounds, were identified in all extracts, along with caffeic and chlorogenic acids, stigmasterol, campesterol and β-sitosterol.
Conclusions. The obtained results support the use of these plant extracts in moderate intensity pain, triggered by both central and peripheral mechanisms. |
| first_indexed | 2024-12-19T14:54:12Z |
| format | Article |
| id | doaj.art-e2cfa05729e24955a8b8c457558eae5f |
| institution | Directory Open Access Journal |
| issn | 2734-8458 2734-8458 |
| language | English |
| last_indexed | 2024-12-19T14:54:12Z |
| publishDate | 2021-03-01 |
| publisher | Romanian Association of Balneology, Editura Balneara |
| record_format | Article |
| series | Balneo and PRM Research Journal |
| spelling | doaj.art-e2cfa05729e24955a8b8c457558eae5f2022-12-21T20:16:45ZengRomanian Association of Balneology, Editura BalnearaBalneo and PRM Research Journal2734-84582734-84582021-03-01121343910.12680/balneo.2021.416Analgesic and anti-inflammatory activity of Artemisia extracts on animal models of nociceptionIVANESCU Bianca0CORCIOVA Andreia1VLASE Laurian2GHELDIU Ana-Maria3MIRON Anca4ABABEI Daniela Carmen5BILD Veronica6“Grigore T. Popa” University of Medicine and Pharmacy, Faculty of Pharmacy, Iași, Romania“Grigore T. Popa” University of Medicine and Pharmacy, Faculty of Pharmacy, Iași, Romania“Iuliu Hatieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Cluj-Napoca, Romania“Iuliu Hatieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Cluj-Napoca, Romania“Grigore T. Popa” University of Medicine and Pharmacy, Faculty of Pharmacy, Iași, Romania“Grigore T. Popa” University of Medicine and Pharmacy, Faculty of Pharmacy, Iași, Romania“Grigore T. Popa” University of Medicine and Pharmacy, Faculty of Pharmacy, Iași, RomaniaIntroduction. The study aims to assess the antinociceptive and anti-inflammatory activity of extracts obtained from indigenous species of wormwood: Artemisia absinthium, A. annua, A. vulgaris and A. pontica. Materials and methods. For these experiments, we employed nociception models using thermal stimulus (hot plate and tail immersion tests, t = 52.5 0C; 30, 60 and 90 minutes testing), chemical stimulus (Zymosan-induced abdominal constriction response test, using distinct lots and testing at 60, 90 and 120 minutes after administration of samples) and pressure stimulus (Randall Selitto test) and an inflammation model for the evaluation of inflammatory edema by Plethysmometer test. Groups of 6 Swiss mice / lot were used, receiving by oral administration the plant extracts suspended in 0.1% CMC-Na. The doses were administered in geometric progression. Chemical analyses were performed by HPLC-MS in order to identify bioactive substances present in extracts: methoxylated flavonoids, sesquiterpene lactones, phytosterols and hydroxycinnamic acids. Results and discussion. All plant extracts showed antinociceptive action on the models with thermal stimulus, as demonstrated by the ED50 values obtained at different test times. In the models of nociception with chemical and mechanical stimulus, models based on inflammatory mediation, the studied fractions have partially proved their antinociceptive action. Regarding the degree of inhibition of inflammatory edema, the highest potency was exhibited by Artemisia pontica extract (86.5% inhibition for the dose of 100 mg/kg). Hispidulin and eupatorin, known anti-inflammatory compounds, were identified in all extracts, along with caffeic and chlorogenic acids, stigmasterol, campesterol and β-sitosterol. Conclusions. The obtained results support the use of these plant extracts in moderate intensity pain, triggered by both central and peripheral mechanisms.http://bioclima.ro/Balneo416.pdfhot-plate testtail immersion testabdominal constrictive responserandall-selitto testinflammatory edema |
| spellingShingle | IVANESCU Bianca CORCIOVA Andreia VLASE Laurian GHELDIU Ana-Maria MIRON Anca ABABEI Daniela Carmen BILD Veronica Analgesic and anti-inflammatory activity of Artemisia extracts on animal models of nociception Balneo and PRM Research Journal hot-plate test tail immersion test abdominal constrictive response randall-selitto test inflammatory edema |
| title | Analgesic and anti-inflammatory activity of Artemisia extracts on animal models of nociception |
| title_full | Analgesic and anti-inflammatory activity of Artemisia extracts on animal models of nociception |
| title_fullStr | Analgesic and anti-inflammatory activity of Artemisia extracts on animal models of nociception |
| title_full_unstemmed | Analgesic and anti-inflammatory activity of Artemisia extracts on animal models of nociception |
| title_short | Analgesic and anti-inflammatory activity of Artemisia extracts on animal models of nociception |
| title_sort | analgesic and anti inflammatory activity of artemisia extracts on animal models of nociception |
| topic | hot-plate test tail immersion test abdominal constrictive response randall-selitto test inflammatory edema |
| url | http://bioclima.ro/Balneo416.pdf |
| work_keys_str_mv | AT ivanescubianca analgesicandantiinflammatoryactivityofartemisiaextractsonanimalmodelsofnociception AT corciovaandreia analgesicandantiinflammatoryactivityofartemisiaextractsonanimalmodelsofnociception AT vlaselaurian analgesicandantiinflammatoryactivityofartemisiaextractsonanimalmodelsofnociception AT gheldiuanamaria analgesicandantiinflammatoryactivityofartemisiaextractsonanimalmodelsofnociception AT mironanca analgesicandantiinflammatoryactivityofartemisiaextractsonanimalmodelsofnociception AT ababeidanielacarmen analgesicandantiinflammatoryactivityofartemisiaextractsonanimalmodelsofnociception AT bildveronica analgesicandantiinflammatoryactivityofartemisiaextractsonanimalmodelsofnociception |