Mechanisms of Fibroblast Growth Factor 21 Adsorption on Macroion Layers: Molecular Dynamics Modeling and Kinetic Measurements
Molecular dynamic modeling and various experimental techniques, including multi-angle dynamic light scattering (MADLS), streaming potential, optical waveguide light spectroscopy (OWLS), quartz crystal microbalance with dissipation (QCM), and atomic force microscopy (AFM), were applied to determine t...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/12/1709 |
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| author | Monika Wasilewska Maria Dąbkowska Agata Pomorska Piotr Batys Bogusław Kowalski Aneta Michna Zbigniew Adamczyk |
| author_facet | Monika Wasilewska Maria Dąbkowska Agata Pomorska Piotr Batys Bogusław Kowalski Aneta Michna Zbigniew Adamczyk |
| author_sort | Monika Wasilewska |
| collection | DOAJ |
| description | Molecular dynamic modeling and various experimental techniques, including multi-angle dynamic light scattering (MADLS), streaming potential, optical waveguide light spectroscopy (OWLS), quartz crystal microbalance with dissipation (QCM), and atomic force microscopy (AFM), were applied to determine the basic physicochemical parameters of fibroblast growth factor 21 in electrolyte solutions. The protein size and shape, cross-section area, dependence of the nominal charge on pH, and isoelectric point of 5.3 were acquired. These data enabled the interpretation of the adsorption kinetics of FGF 21 on bare and macrocation-covered silica investigated by OWLS and QCM. It was confirmed that the protein molecules irreversibly adsorbed on the latter substrate, forming layers with controlled coverage up to 0.8 mg m<sup>−2</sup>, while their adsorption on bare silica was much smaller. The viability of two cell lines, CHO-K1 and L-929, on both bare and macrocation/FGF 21-covered substrates was also determined. It is postulated that the acquired results can serve as useful reference systems for designing complexes that can extend the half-life of FGF 21 in its active state. |
| first_indexed | 2024-03-08T20:58:12Z |
| format | Article |
| id | doaj.art-e2d01bd3e4d24dff99697aeba60e6bd1 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-08T20:58:12Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-e2d01bd3e4d24dff99697aeba60e6bd12023-12-22T13:55:51ZengMDPI AGBiomolecules2218-273X2023-11-011312170910.3390/biom13121709Mechanisms of Fibroblast Growth Factor 21 Adsorption on Macroion Layers: Molecular Dynamics Modeling and Kinetic MeasurementsMonika Wasilewska0Maria Dąbkowska1Agata Pomorska2Piotr Batys3Bogusław Kowalski4Aneta Michna5Zbigniew Adamczyk6Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, PolandIndependent Laboratory of Pharmacokinetic and Clinical Pharmacy, Pomeranian Medical University, Rybacka 1, 70-204 Szczecin, PolandJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, PolandJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, PolandIndependent Laboratory of Pharmacokinetic and Clinical Pharmacy, Pomeranian Medical University, Rybacka 1, 70-204 Szczecin, PolandJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, PolandJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, PolandMolecular dynamic modeling and various experimental techniques, including multi-angle dynamic light scattering (MADLS), streaming potential, optical waveguide light spectroscopy (OWLS), quartz crystal microbalance with dissipation (QCM), and atomic force microscopy (AFM), were applied to determine the basic physicochemical parameters of fibroblast growth factor 21 in electrolyte solutions. The protein size and shape, cross-section area, dependence of the nominal charge on pH, and isoelectric point of 5.3 were acquired. These data enabled the interpretation of the adsorption kinetics of FGF 21 on bare and macrocation-covered silica investigated by OWLS and QCM. It was confirmed that the protein molecules irreversibly adsorbed on the latter substrate, forming layers with controlled coverage up to 0.8 mg m<sup>−2</sup>, while their adsorption on bare silica was much smaller. The viability of two cell lines, CHO-K1 and L-929, on both bare and macrocation/FGF 21-covered substrates was also determined. It is postulated that the acquired results can serve as useful reference systems for designing complexes that can extend the half-life of FGF 21 in its active state.https://www.mdpi.com/2218-273X/13/12/1709fibroblast growth factor 21poly(diallyldimethylammonium chloride)molecular dynamics modelingadsorption of growth factoroptical waveguide lightmode spectroscopyquartz crystal microbalance |
| spellingShingle | Monika Wasilewska Maria Dąbkowska Agata Pomorska Piotr Batys Bogusław Kowalski Aneta Michna Zbigniew Adamczyk Mechanisms of Fibroblast Growth Factor 21 Adsorption on Macroion Layers: Molecular Dynamics Modeling and Kinetic Measurements Biomolecules fibroblast growth factor 21 poly(diallyldimethylammonium chloride) molecular dynamics modeling adsorption of growth factor optical waveguide lightmode spectroscopy quartz crystal microbalance |
| title | Mechanisms of Fibroblast Growth Factor 21 Adsorption on Macroion Layers: Molecular Dynamics Modeling and Kinetic Measurements |
| title_full | Mechanisms of Fibroblast Growth Factor 21 Adsorption on Macroion Layers: Molecular Dynamics Modeling and Kinetic Measurements |
| title_fullStr | Mechanisms of Fibroblast Growth Factor 21 Adsorption on Macroion Layers: Molecular Dynamics Modeling and Kinetic Measurements |
| title_full_unstemmed | Mechanisms of Fibroblast Growth Factor 21 Adsorption on Macroion Layers: Molecular Dynamics Modeling and Kinetic Measurements |
| title_short | Mechanisms of Fibroblast Growth Factor 21 Adsorption on Macroion Layers: Molecular Dynamics Modeling and Kinetic Measurements |
| title_sort | mechanisms of fibroblast growth factor 21 adsorption on macroion layers molecular dynamics modeling and kinetic measurements |
| topic | fibroblast growth factor 21 poly(diallyldimethylammonium chloride) molecular dynamics modeling adsorption of growth factor optical waveguide lightmode spectroscopy quartz crystal microbalance |
| url | https://www.mdpi.com/2218-273X/13/12/1709 |
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