A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer
Multiplex assays often rely on expensive sensors incorporating covalently linked fluorescent dyes. Herein, we developed a self-assembling aptamer-based multiplex assay. This multiplex approach utilizes a previously established split aptamer sensor in conjugation with a novel split aptamer sensor bas...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Sensors and Actuators Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053922000522 |
| _version_ | 1828120589387169792 |
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| author | Martin R． O'Steen Dmitry M．Kolpashchikov |
| author_facet | Martin R． O'Steen Dmitry M．Kolpashchikov |
| author_sort | Martin R． O'Steen |
| collection | DOAJ |
| description | Multiplex assays often rely on expensive sensors incorporating covalently linked fluorescent dyes. Herein, we developed a self-assembling aptamer-based multiplex assay. This multiplex approach utilizes a previously established split aptamer sensor in conjugation with a novel split aptamer sensor based upon a malachite green DNA aptamer. This system was capable of simultaneous fluorescent detection of two SARS COVID-19-related sequences in one sample with individual sensors that possesses a limit of detection (LOD) in the low nM range. Optimization of the Split Malachite Green (SMG) sensor yielded a minimized aptamer construct, Mini-MG, capable of inducing fluorescence of malachite green in both a DNA hairpin and sensor format. |
| first_indexed | 2024-04-11T14:07:28Z |
| format | Article |
| id | doaj.art-e2d08cb92b54456d92b5fe5f76f8e2f8 |
| institution | Directory Open Access Journal |
| issn | 2666-0539 |
| language | English |
| last_indexed | 2024-04-11T14:07:28Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj.art-e2d08cb92b54456d92b5fe5f76f8e2f82022-12-22T04:19:49ZengElsevierSensors and Actuators Reports2666-05392022-11-014100125A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamerMartin R． O'Steen0Dmitry M．Kolpashchikov1Chemistry Department, University of Central Florida, Orlando, FL, USA; Corresponding author.Chemistry Department, University of Central Florida, Orlando, FL, USA; Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA; National Center for Forensic Science, University of Central Florida, Orlando, FL, USAMultiplex assays often rely on expensive sensors incorporating covalently linked fluorescent dyes. Herein, we developed a self-assembling aptamer-based multiplex assay. This multiplex approach utilizes a previously established split aptamer sensor in conjugation with a novel split aptamer sensor based upon a malachite green DNA aptamer. This system was capable of simultaneous fluorescent detection of two SARS COVID-19-related sequences in one sample with individual sensors that possesses a limit of detection (LOD) in the low nM range. Optimization of the Split Malachite Green (SMG) sensor yielded a minimized aptamer construct, Mini-MG, capable of inducing fluorescence of malachite green in both a DNA hairpin and sensor format.http://www.sciencedirect.com/science/article/pii/S2666053922000522AptamerBiosensorsSplit probeMiniaturizationMalachite greenDNA |
| spellingShingle | Martin R． O'Steen Dmitry M．Kolpashchikov A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer Sensors and Actuators Reports Aptamer Biosensors Split probe Miniaturization Malachite green DNA |
| title | A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer |
| title_full | A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer |
| title_fullStr | A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer |
| title_full_unstemmed | A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer |
| title_short | A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer |
| title_sort | self assembling split aptamer multiplex assay for sars covid19 and miniaturization of a malachite green dna based aptamer |
| topic | Aptamer Biosensors Split probe Miniaturization Malachite green DNA |
| url | http://www.sciencedirect.com/science/article/pii/S2666053922000522 |
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