Unveiling the Anti-Biofilm Property of Hydroxyapatite on <i>Pseudomonas aeruginosa</i>: Synthesis and Strategy
Biofilm-related nosocomial infections may cause a wide range of life-threatening infections. In this regard, <i>Pseudomonas aeruginosa</i> biofilm is becoming a serious health burden due to its capability to develop resistance to natural and synthetic drugs. The utilization of nanopartic...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/2/463 |
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| author | Davoodbasha MubarakAli Kannappan Arunachalam Murugan Lakshmanan Bazigha Badar Jung-Wan Kim Sang-Yul Lee |
| author_facet | Davoodbasha MubarakAli Kannappan Arunachalam Murugan Lakshmanan Bazigha Badar Jung-Wan Kim Sang-Yul Lee |
| author_sort | Davoodbasha MubarakAli |
| collection | DOAJ |
| description | Biofilm-related nosocomial infections may cause a wide range of life-threatening infections. In this regard, <i>Pseudomonas aeruginosa</i> biofilm is becoming a serious health burden due to its capability to develop resistance to natural and synthetic drugs. The utilization of nanoparticles that inhibit biofilm formation is one of the major strategies to control infections caused by biofilm-forming pathogens. Hydroxyapatite (HA) is a synthetic ceramic material having properties similar to natural bones. Herein, a co-precipitation method followed by microwave treatment was used to synthesize HA nanoparticles (HANPs). The resulting HANPs were characterized using X-ray diffraction and transmission electron microscopy. Then, their antibiofilm properties against <i>P. aeruginosa</i> ATCC 10145 were examined in vitro. The needle-shaped HANPs were 30 and 90 nm long in width and length, respectively. The synthesized HANPs inhibited the biofilm formation of <i>P. aeruginosa</i> ATCC 10145 in a concentration-dependent manner, which was validated by light and confocal laser scanning microscopy. Hence, this study demonstrated that HANPs could be used to control the biofilm-related infections of <i>P. aeruginosa</i>. |
| first_indexed | 2024-03-11T08:17:25Z |
| format | Article |
| id | doaj.art-e2d62cab37dd41a98664b24455bab629 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T08:17:25Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-e2d62cab37dd41a98664b24455bab6292023-11-16T22:40:17ZengMDPI AGPharmaceutics1999-49232023-01-0115246310.3390/pharmaceutics15020463Unveiling the Anti-Biofilm Property of Hydroxyapatite on <i>Pseudomonas aeruginosa</i>: Synthesis and StrategyDavoodbasha MubarakAli0Kannappan Arunachalam1Murugan Lakshmanan2Bazigha Badar3Jung-Wan Kim4Sang-Yul Lee5School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai 620048, IndiaState Key Laboratory of Microbial Metabolism, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai 620048, IndiaDepartment of Environmental Science, Amar Singh College, Cluster University Srinagar, Srinagar 190008, IndiaCentre for Surface Coating and Technology, Department of Material Engineering, Korea Aerospace University, Goyang 10540, Republic of KoreaCentre for Surface Coating and Technology, Department of Material Engineering, Korea Aerospace University, Goyang 10540, Republic of KoreaBiofilm-related nosocomial infections may cause a wide range of life-threatening infections. In this regard, <i>Pseudomonas aeruginosa</i> biofilm is becoming a serious health burden due to its capability to develop resistance to natural and synthetic drugs. The utilization of nanoparticles that inhibit biofilm formation is one of the major strategies to control infections caused by biofilm-forming pathogens. Hydroxyapatite (HA) is a synthetic ceramic material having properties similar to natural bones. Herein, a co-precipitation method followed by microwave treatment was used to synthesize HA nanoparticles (HANPs). The resulting HANPs were characterized using X-ray diffraction and transmission electron microscopy. Then, their antibiofilm properties against <i>P. aeruginosa</i> ATCC 10145 were examined in vitro. The needle-shaped HANPs were 30 and 90 nm long in width and length, respectively. The synthesized HANPs inhibited the biofilm formation of <i>P. aeruginosa</i> ATCC 10145 in a concentration-dependent manner, which was validated by light and confocal laser scanning microscopy. Hence, this study demonstrated that HANPs could be used to control the biofilm-related infections of <i>P. aeruginosa</i>.https://www.mdpi.com/1999-4923/15/2/463hydroxyapatiteanti-biofilm<i>Pseudomonas aeruginosa</i>nanoparticles |
| spellingShingle | Davoodbasha MubarakAli Kannappan Arunachalam Murugan Lakshmanan Bazigha Badar Jung-Wan Kim Sang-Yul Lee Unveiling the Anti-Biofilm Property of Hydroxyapatite on <i>Pseudomonas aeruginosa</i>: Synthesis and Strategy Pharmaceutics hydroxyapatite anti-biofilm <i>Pseudomonas aeruginosa</i> nanoparticles |
| title | Unveiling the Anti-Biofilm Property of Hydroxyapatite on <i>Pseudomonas aeruginosa</i>: Synthesis and Strategy |
| title_full | Unveiling the Anti-Biofilm Property of Hydroxyapatite on <i>Pseudomonas aeruginosa</i>: Synthesis and Strategy |
| title_fullStr | Unveiling the Anti-Biofilm Property of Hydroxyapatite on <i>Pseudomonas aeruginosa</i>: Synthesis and Strategy |
| title_full_unstemmed | Unveiling the Anti-Biofilm Property of Hydroxyapatite on <i>Pseudomonas aeruginosa</i>: Synthesis and Strategy |
| title_short | Unveiling the Anti-Biofilm Property of Hydroxyapatite on <i>Pseudomonas aeruginosa</i>: Synthesis and Strategy |
| title_sort | unveiling the anti biofilm property of hydroxyapatite on i pseudomonas aeruginosa i synthesis and strategy |
| topic | hydroxyapatite anti-biofilm <i>Pseudomonas aeruginosa</i> nanoparticles |
| url | https://www.mdpi.com/1999-4923/15/2/463 |
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