Local intra-articular injection of rapamycin inhibits NLRP3 activity and prevents osteoarthritis in mouse DMM models
This study investigated the influence of autophagy on the expression of Collagen type II and light chain 3 (LC-3) in the articular cartilage of osteoarthritis (OA) models. The expression of OA associated biomarkers namely Matrix metalloproteinase (MMP-13), NOD-, LRR- and pyrin domain-containing 3 (N...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-05-01
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| Series: | Autoimmunity |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08916934.2019.1643844 |
| _version_ | 1797684770585444352 |
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| author | Gang Xu Jian Wang Long Ma Xin Zhao Wen Luo Qunhua Jin |
| author_facet | Gang Xu Jian Wang Long Ma Xin Zhao Wen Luo Qunhua Jin |
| author_sort | Gang Xu |
| collection | DOAJ |
| description | This study investigated the influence of autophagy on the expression of Collagen type II and light chain 3 (LC-3) in the articular cartilage of osteoarthritis (OA) models. The expression of OA associated biomarkers namely Matrix metalloproteinase (MMP-13), NOD-, LRR- and pyrin domain-containing 3 (NLRP3) induced by destabilizing the medial meniscus operation (DMM) were also investigated. A total of 60 C57BL/6 mice were divided into (1) control; (2) DMM2; (3) DMM8; (4) rapamycin 2 weeks; and (5) rapamycin 8 weeks groups. Saffranin O-Fast green staining, histomorphometry and immunohistochemical methods were used for analysis. In the DMM group, the expression of the OA biomarkers MMP-13, NLRP3 significantly increased, whilst Collagen II and LC-3B levels were significantly lower than other experimental groups. We hypothesized that NLRP3 inhibits autophagy activation and delays disease progression. |
| first_indexed | 2024-03-12T00:34:36Z |
| format | Article |
| id | doaj.art-e2da3554adb44bf1be0d3467409bee4d |
| institution | Directory Open Access Journal |
| issn | 0891-6934 1607-842X |
| language | English |
| last_indexed | 2024-03-12T00:34:36Z |
| publishDate | 2019-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autoimmunity |
| spelling | doaj.art-e2da3554adb44bf1be0d3467409bee4d2023-09-15T10:01:08ZengTaylor & Francis GroupAutoimmunity0891-69341607-842X2019-05-0152416817510.1080/08916934.2019.16438441643844Local intra-articular injection of rapamycin inhibits NLRP3 activity and prevents osteoarthritis in mouse DMM modelsGang Xu0Jian Wang1Long Ma2Xin Zhao3Wen Luo4Qunhua Jin5The School of Clinical Medicine, Ningxia Medical UniversityThe School of Clinical Medicine, Ningxia Medical UniversityThe School of Clinical Medicine, Ningxia Medical UniversityThe School of Clinical Medicine, Ningxia Medical UniversityThe School of Clinical Medicine, Ningxia Medical UniversityThe School of Clinical Medicine, Ningxia Medical UniversityThis study investigated the influence of autophagy on the expression of Collagen type II and light chain 3 (LC-3) in the articular cartilage of osteoarthritis (OA) models. The expression of OA associated biomarkers namely Matrix metalloproteinase (MMP-13), NOD-, LRR- and pyrin domain-containing 3 (NLRP3) induced by destabilizing the medial meniscus operation (DMM) were also investigated. A total of 60 C57BL/6 mice were divided into (1) control; (2) DMM2; (3) DMM8; (4) rapamycin 2 weeks; and (5) rapamycin 8 weeks groups. Saffranin O-Fast green staining, histomorphometry and immunohistochemical methods were used for analysis. In the DMM group, the expression of the OA biomarkers MMP-13, NLRP3 significantly increased, whilst Collagen II and LC-3B levels were significantly lower than other experimental groups. We hypothesized that NLRP3 inhibits autophagy activation and delays disease progression.http://dx.doi.org/10.1080/08916934.2019.1643844rapamycinautophagynlrp3oadmm |
| spellingShingle | Gang Xu Jian Wang Long Ma Xin Zhao Wen Luo Qunhua Jin Local intra-articular injection of rapamycin inhibits NLRP3 activity and prevents osteoarthritis in mouse DMM models Autoimmunity rapamycin autophagy nlrp3 oa dmm |
| title | Local intra-articular injection of rapamycin inhibits NLRP3 activity and prevents osteoarthritis in mouse DMM models |
| title_full | Local intra-articular injection of rapamycin inhibits NLRP3 activity and prevents osteoarthritis in mouse DMM models |
| title_fullStr | Local intra-articular injection of rapamycin inhibits NLRP3 activity and prevents osteoarthritis in mouse DMM models |
| title_full_unstemmed | Local intra-articular injection of rapamycin inhibits NLRP3 activity and prevents osteoarthritis in mouse DMM models |
| title_short | Local intra-articular injection of rapamycin inhibits NLRP3 activity and prevents osteoarthritis in mouse DMM models |
| title_sort | local intra articular injection of rapamycin inhibits nlrp3 activity and prevents osteoarthritis in mouse dmm models |
| topic | rapamycin autophagy nlrp3 oa dmm |
| url | http://dx.doi.org/10.1080/08916934.2019.1643844 |
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