Interactions of Tea-Derived Catechin Gallates with Bacterial Pathogens
Green tea-derived galloylated catechins have weak direct antibacterial activity against both Gram-positive and Gram-negative bacterial pathogens and are able to phenotypically transform, at moderate concentrations, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) clonal pathogen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/8/1986 |
| _version_ | 1797569813360410624 |
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| author | Peter W. Taylor |
| author_facet | Peter W. Taylor |
| author_sort | Peter W. Taylor |
| collection | DOAJ |
| description | Green tea-derived galloylated catechins have weak direct antibacterial activity against both Gram-positive and Gram-negative bacterial pathogens and are able to phenotypically transform, at moderate concentrations, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) clonal pathogens from full β-lactam resistance (minimum inhibitory concentration 256–512 mg/L) to complete susceptibility (~1 mg/L). Reversible conversion to susceptibility follows intercalation of these compounds into the bacterial cytoplasmic membrane, eliciting dispersal of the proteins associated with continued cell wall peptidoglycan synthesis in the presence of β-lactam antibiotics. The molecules penetrate deep within the hydrophobic core of the lipid palisade to force a reconfiguration of cytoplasmic membrane architecture. The catechin gallate-induced staphylococcal phenotype is complex, reflecting perturbation of an essential bacterial organelle, and includes prevention and inhibition of biofilm formation, disruption of secretion of virulence-related proteins, dissipation of halotolerance, cell wall thickening and cell aggregation and poor separation of daughter cells during cell division. These features are associated with the reduction of capacity of potential pathogens to cause lethal, difficult-to-treat infections and could, in combination with β-lactam agents that have lost therapeutic efficacy due to the emergence of antibiotic resistance, form the basis of a new approach to the treatment of staphylococcal infections. |
| first_indexed | 2024-03-10T20:16:52Z |
| format | Article |
| id | doaj.art-e2dda19dce6e45c8a5391d6b7db8c3d4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:16:52Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e2dda19dce6e45c8a5391d6b7db8c3d42023-11-19T22:32:38ZengMDPI AGMolecules1420-30492020-04-01258198610.3390/molecules25081986Interactions of Tea-Derived Catechin Gallates with Bacterial PathogensPeter W. Taylor0School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UKGreen tea-derived galloylated catechins have weak direct antibacterial activity against both Gram-positive and Gram-negative bacterial pathogens and are able to phenotypically transform, at moderate concentrations, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) clonal pathogens from full β-lactam resistance (minimum inhibitory concentration 256–512 mg/L) to complete susceptibility (~1 mg/L). Reversible conversion to susceptibility follows intercalation of these compounds into the bacterial cytoplasmic membrane, eliciting dispersal of the proteins associated with continued cell wall peptidoglycan synthesis in the presence of β-lactam antibiotics. The molecules penetrate deep within the hydrophobic core of the lipid palisade to force a reconfiguration of cytoplasmic membrane architecture. The catechin gallate-induced staphylococcal phenotype is complex, reflecting perturbation of an essential bacterial organelle, and includes prevention and inhibition of biofilm formation, disruption of secretion of virulence-related proteins, dissipation of halotolerance, cell wall thickening and cell aggregation and poor separation of daughter cells during cell division. These features are associated with the reduction of capacity of potential pathogens to cause lethal, difficult-to-treat infections and could, in combination with β-lactam agents that have lost therapeutic efficacy due to the emergence of antibiotic resistance, form the basis of a new approach to the treatment of staphylococcal infections.https://www.mdpi.com/1420-3049/25/8/1986catechin gallates<i>Staphylococcus aureus</i>β-lactam antibioticscytoplasmic membranepenicillin-binding proteinsbacterial cell wall |
| spellingShingle | Peter W. Taylor Interactions of Tea-Derived Catechin Gallates with Bacterial Pathogens Molecules catechin gallates <i>Staphylococcus aureus</i> β-lactam antibiotics cytoplasmic membrane penicillin-binding proteins bacterial cell wall |
| title | Interactions of Tea-Derived Catechin Gallates with Bacterial Pathogens |
| title_full | Interactions of Tea-Derived Catechin Gallates with Bacterial Pathogens |
| title_fullStr | Interactions of Tea-Derived Catechin Gallates with Bacterial Pathogens |
| title_full_unstemmed | Interactions of Tea-Derived Catechin Gallates with Bacterial Pathogens |
| title_short | Interactions of Tea-Derived Catechin Gallates with Bacterial Pathogens |
| title_sort | interactions of tea derived catechin gallates with bacterial pathogens |
| topic | catechin gallates <i>Staphylococcus aureus</i> β-lactam antibiotics cytoplasmic membrane penicillin-binding proteins bacterial cell wall |
| url | https://www.mdpi.com/1420-3049/25/8/1986 |
| work_keys_str_mv | AT peterwtaylor interactionsofteaderivedcatechingallateswithbacterialpathogens |