Role of Th17 Cytokines in the Liver’s Immune Response during Fatal Yellow Fever: Triggering Cell Damage Mechanisms
Yellow fever (YF) is an infectious and acute viral haemorrhagic disease that triggers a cascade of host immune responses. We investigated the Th17 cytokine profile in the liver tissue of patients with fatal YF. Liver tissue samples were collected from 26 deceased patients, including 21 YF-positive a...
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/13/2053 |
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| author | Marcos Luiz Gaia Carvalho Luiz Fábio Magno Falcão Jeferson da Costa Lopes Caio Cesar Henriques Mendes Fábio Alves Olímpio Vanessa do Socorro Cabral Miranda Lais Carneiro dos Santos Daniel Dias Pinheiro de Moraes Marcos Virgilio Bertonsin Filho Luccas Delgado da Costa Raimunda do Socorro da Silva Azevedo Ana Cecília Ribeiro Cruz Vanessa Costa Alves Galúcio Lívia Caricio Martins Maria Irma Seixas Duarte Arnaldo Jorge Martins Filho Jorge Rodrigues de Sousa Pedro Fernando da Costa Vasconcelos Juarez Antônio Simões Quaresma |
| author_facet | Marcos Luiz Gaia Carvalho Luiz Fábio Magno Falcão Jeferson da Costa Lopes Caio Cesar Henriques Mendes Fábio Alves Olímpio Vanessa do Socorro Cabral Miranda Lais Carneiro dos Santos Daniel Dias Pinheiro de Moraes Marcos Virgilio Bertonsin Filho Luccas Delgado da Costa Raimunda do Socorro da Silva Azevedo Ana Cecília Ribeiro Cruz Vanessa Costa Alves Galúcio Lívia Caricio Martins Maria Irma Seixas Duarte Arnaldo Jorge Martins Filho Jorge Rodrigues de Sousa Pedro Fernando da Costa Vasconcelos Juarez Antônio Simões Quaresma |
| author_sort | Marcos Luiz Gaia Carvalho |
| collection | DOAJ |
| description | Yellow fever (YF) is an infectious and acute viral haemorrhagic disease that triggers a cascade of host immune responses. We investigated the Th17 cytokine profile in the liver tissue of patients with fatal YF. Liver tissue samples were collected from 26 deceased patients, including 21 YF-positive and 5 flavivirus-negative patients, with preserved hepatic parenchyma architecture, who died of other causes. Histopathological and immunohistochemical analysis were performed on the liver samples to evaluate the Th17 profiles (ROR-γ, STAT3, IL-6, TGF-β, IL-17A, and IL-23). Substantial differences were found in the expression levels of these markers between the patients with fatal YF and controls. A predominant expression of Th17 cytokine markers was observed in the midzonal region of the YF cases, the most affected area in the liver acinus, compared with the controls. Histopathological changes in the hepatic parenchyma revealed cellular damage characterised mainly by the presence of inflammatory cell infiltrates, Councilman bodies (apoptotic cells), micro/macrovesicular steatosis, and lytic and coagulative necrosis. Hence, Th17 cytokines play a pivotal role in the immunopathogenesis of YF and contribute markedly to triggering cell damage in patients with fatal disease outcomes. |
| first_indexed | 2024-03-09T22:02:03Z |
| format | Article |
| id | doaj.art-e2e7466fee0d4aa1a9c64064a95845da |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T22:02:03Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-e2e7466fee0d4aa1a9c64064a95845da2023-11-23T19:48:42ZengMDPI AGCells2073-44092022-06-011113205310.3390/cells11132053Role of Th17 Cytokines in the Liver’s Immune Response during Fatal Yellow Fever: Triggering Cell Damage MechanismsMarcos Luiz Gaia Carvalho0Luiz Fábio Magno Falcão1Jeferson da Costa Lopes2Caio Cesar Henriques Mendes3Fábio Alves Olímpio4Vanessa do Socorro Cabral Miranda5Lais Carneiro dos Santos6Daniel Dias Pinheiro de Moraes7Marcos Virgilio Bertonsin Filho8Luccas Delgado da Costa9Raimunda do Socorro da Silva Azevedo10Ana Cecília Ribeiro Cruz11Vanessa Costa Alves Galúcio12Lívia Caricio Martins13Maria Irma Seixas Duarte14Arnaldo Jorge Martins Filho15Jorge Rodrigues de Sousa16Pedro Fernando da Costa Vasconcelos17Juarez Antônio Simões Quaresma18Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, PA, BrazilCentro de Ciências Biológicas e da Saúde, Universidade do Estado do Pará, Belém 66087-662, PA, BrazilInstituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, PA, BrazilCentro de Ciências Biológicas e da Saúde, Universidade do Estado do Pará, Belém 66087-662, PA, BrazilNúcleo de Medicina Tropical, Universidade Federal do Pará, Belém 66055-240, PA, BrazilInstituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, PA, BrazilInstituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, PA, BrazilNúcleo de Medicina Tropical, Universidade Federal do Pará, Belém 66055-240, PA, BrazilNúcleo de Medicina Tropical, Universidade Federal do Pará, Belém 66055-240, PA, BrazilNúcleo de Medicina Tropical, Universidade Federal do Pará, Belém 66055-240, PA, BrazilInstituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, PA, BrazilInstituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, PA, BrazilCurso de Biomedicina, Faculdade Cosmopolita, Belém 66615-005, PA, BrazilInstituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, PA, BrazilFaculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, SP, BrazilInstituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, PA, BrazilInstituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, PA, BrazilInstituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, PA, BrazilCentro de Ciências Biológicas e da Saúde, Universidade do Estado do Pará, Belém 66087-662, PA, BrazilYellow fever (YF) is an infectious and acute viral haemorrhagic disease that triggers a cascade of host immune responses. We investigated the Th17 cytokine profile in the liver tissue of patients with fatal YF. Liver tissue samples were collected from 26 deceased patients, including 21 YF-positive and 5 flavivirus-negative patients, with preserved hepatic parenchyma architecture, who died of other causes. Histopathological and immunohistochemical analysis were performed on the liver samples to evaluate the Th17 profiles (ROR-γ, STAT3, IL-6, TGF-β, IL-17A, and IL-23). Substantial differences were found in the expression levels of these markers between the patients with fatal YF and controls. A predominant expression of Th17 cytokine markers was observed in the midzonal region of the YF cases, the most affected area in the liver acinus, compared with the controls. Histopathological changes in the hepatic parenchyma revealed cellular damage characterised mainly by the presence of inflammatory cell infiltrates, Councilman bodies (apoptotic cells), micro/macrovesicular steatosis, and lytic and coagulative necrosis. Hence, Th17 cytokines play a pivotal role in the immunopathogenesis of YF and contribute markedly to triggering cell damage in patients with fatal disease outcomes.https://www.mdpi.com/2073-4409/11/13/2053yellow feverTh17 profilecell damageliver immune response |
| spellingShingle | Marcos Luiz Gaia Carvalho Luiz Fábio Magno Falcão Jeferson da Costa Lopes Caio Cesar Henriques Mendes Fábio Alves Olímpio Vanessa do Socorro Cabral Miranda Lais Carneiro dos Santos Daniel Dias Pinheiro de Moraes Marcos Virgilio Bertonsin Filho Luccas Delgado da Costa Raimunda do Socorro da Silva Azevedo Ana Cecília Ribeiro Cruz Vanessa Costa Alves Galúcio Lívia Caricio Martins Maria Irma Seixas Duarte Arnaldo Jorge Martins Filho Jorge Rodrigues de Sousa Pedro Fernando da Costa Vasconcelos Juarez Antônio Simões Quaresma Role of Th17 Cytokines in the Liver’s Immune Response during Fatal Yellow Fever: Triggering Cell Damage Mechanisms Cells yellow fever Th17 profile cell damage liver immune response |
| title | Role of Th17 Cytokines in the Liver’s Immune Response during Fatal Yellow Fever: Triggering Cell Damage Mechanisms |
| title_full | Role of Th17 Cytokines in the Liver’s Immune Response during Fatal Yellow Fever: Triggering Cell Damage Mechanisms |
| title_fullStr | Role of Th17 Cytokines in the Liver’s Immune Response during Fatal Yellow Fever: Triggering Cell Damage Mechanisms |
| title_full_unstemmed | Role of Th17 Cytokines in the Liver’s Immune Response during Fatal Yellow Fever: Triggering Cell Damage Mechanisms |
| title_short | Role of Th17 Cytokines in the Liver’s Immune Response during Fatal Yellow Fever: Triggering Cell Damage Mechanisms |
| title_sort | role of th17 cytokines in the liver s immune response during fatal yellow fever triggering cell damage mechanisms |
| topic | yellow fever Th17 profile cell damage liver immune response |
| url | https://www.mdpi.com/2073-4409/11/13/2053 |
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