<i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> Virulence Factors Are Involved in Resistance to Plant-Derived Antimicrobials during Infection
Bacteria are exposed to and tolerate diverse and potentially toxic compounds in the natural environment. While efflux transporters are generally thought to involve bacterial antibiotic resistance in vitro, their contributions to plant bacterial virulence have so far been poorly understood. <i>...
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MDPI AG
2022-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/13/1742 |
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| author | Nanami Sakata Takumi Haraguchi Shunsuke Masuo Takako Ishiga Yasuhiro Ishiga |
| author_facet | Nanami Sakata Takumi Haraguchi Shunsuke Masuo Takako Ishiga Yasuhiro Ishiga |
| author_sort | Nanami Sakata |
| collection | DOAJ |
| description | Bacteria are exposed to and tolerate diverse and potentially toxic compounds in the natural environment. While efflux transporters are generally thought to involve bacterial antibiotic resistance in vitro, their contributions to plant bacterial virulence have so far been poorly understood. <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> (<i>Pcal</i>) is a causal agent of bacterial blight of Brassicaceae. We here demonstrated that NU19, which is mutated in the resistance-nodulation-cell division (RND) transporter encoded gene, showed reduced virulence on cabbage compared to WT, indicating that the RND transporter contributes to <i>Pcal</i> virulence on cabbage. We also demonstrated that brassinin biosynthesis was induced after <i>Pcal</i> infection. Additionally, the RND transporter was involved in resistance to plant-derived antimicrobials and antibiotics, including the cabbage phytoalexin brassinin. These results suggest that the RND transporter extrudes plant-derived antimicrobials and contributes to <i>Pcal</i> virulence. We also found that the RND transporter contributes to <i>Pcal</i> virulence on Brassicaceae and tomato, but not on oat. These results suggest that the RND transporter contributes to <i>Pcal</i> virulence differentially depending on the host-plant species. Lastly, our expression-profile analysis indicated that the type-three secretion system (TTSS), which is essential for pathogenesis, is also involved in suppressing brassinin biosynthesis. Taken together, our results suggest that several <i>Pcal</i> virulence factors are involved in resistance to plant-derived antimicrobials and bacterial survival during infection. |
| first_indexed | 2024-03-09T12:40:17Z |
| format | Article |
| id | doaj.art-e2efcb0fb1f54973972b10ad3d51051f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T12:40:17Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-e2efcb0fb1f54973972b10ad3d51051f2023-11-30T22:19:48ZengMDPI AGPlants2223-77472022-06-011113174210.3390/plants11131742<i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> Virulence Factors Are Involved in Resistance to Plant-Derived Antimicrobials during InfectionNanami Sakata0Takumi Haraguchi1Shunsuke Masuo2Takako Ishiga3Yasuhiro Ishiga4Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Ibaraki, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Ibaraki, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Ibaraki, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Ibaraki, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Ibaraki, JapanBacteria are exposed to and tolerate diverse and potentially toxic compounds in the natural environment. While efflux transporters are generally thought to involve bacterial antibiotic resistance in vitro, their contributions to plant bacterial virulence have so far been poorly understood. <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> (<i>Pcal</i>) is a causal agent of bacterial blight of Brassicaceae. We here demonstrated that NU19, which is mutated in the resistance-nodulation-cell division (RND) transporter encoded gene, showed reduced virulence on cabbage compared to WT, indicating that the RND transporter contributes to <i>Pcal</i> virulence on cabbage. We also demonstrated that brassinin biosynthesis was induced after <i>Pcal</i> infection. Additionally, the RND transporter was involved in resistance to plant-derived antimicrobials and antibiotics, including the cabbage phytoalexin brassinin. These results suggest that the RND transporter extrudes plant-derived antimicrobials and contributes to <i>Pcal</i> virulence. We also found that the RND transporter contributes to <i>Pcal</i> virulence on Brassicaceae and tomato, but not on oat. These results suggest that the RND transporter contributes to <i>Pcal</i> virulence differentially depending on the host-plant species. Lastly, our expression-profile analysis indicated that the type-three secretion system (TTSS), which is essential for pathogenesis, is also involved in suppressing brassinin biosynthesis. Taken together, our results suggest that several <i>Pcal</i> virulence factors are involved in resistance to plant-derived antimicrobials and bacterial survival during infection.https://www.mdpi.com/2223-7747/11/13/1742<i>Pseudomonas cannabina</i> pv. <i>alisalensis</i>resistance-nodulation-cell division transportertype-three secretion systemphytoalexinbrassininglucosinolate |
| spellingShingle | Nanami Sakata Takumi Haraguchi Shunsuke Masuo Takako Ishiga Yasuhiro Ishiga <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> Virulence Factors Are Involved in Resistance to Plant-Derived Antimicrobials during Infection Plants <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> resistance-nodulation-cell division transporter type-three secretion system phytoalexin brassinin glucosinolate |
| title | <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> Virulence Factors Are Involved in Resistance to Plant-Derived Antimicrobials during Infection |
| title_full | <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> Virulence Factors Are Involved in Resistance to Plant-Derived Antimicrobials during Infection |
| title_fullStr | <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> Virulence Factors Are Involved in Resistance to Plant-Derived Antimicrobials during Infection |
| title_full_unstemmed | <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> Virulence Factors Are Involved in Resistance to Plant-Derived Antimicrobials during Infection |
| title_short | <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> Virulence Factors Are Involved in Resistance to Plant-Derived Antimicrobials during Infection |
| title_sort | i pseudomonas cannabina i pv i alisalensis i virulence factors are involved in resistance to plant derived antimicrobials during infection |
| topic | <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> resistance-nodulation-cell division transporter type-three secretion system phytoalexin brassinin glucosinolate |
| url | https://www.mdpi.com/2223-7747/11/13/1742 |
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