Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense Response
Maize is one of the major crops in the world; however, diseases caused by various pathogens seriously affect its yield and quality. The maize Rp1-D21 mutant (mt) caused by the intragenic recombination between two nucleotide-binding, leucine-rich repeat (NLR) proteins, exhibits autoactive hypersensit...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.738261/full |
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| author | Chunxia Ge Chunxia Ge Yi-Ge Wang Shouping Lu Xiang Yu Zhao Bing-Kai Hou Peter J. Balint-Kurti Peter J. Balint-Kurti Guan-Feng Wang |
| author_facet | Chunxia Ge Chunxia Ge Yi-Ge Wang Shouping Lu Xiang Yu Zhao Bing-Kai Hou Peter J. Balint-Kurti Peter J. Balint-Kurti Guan-Feng Wang |
| author_sort | Chunxia Ge |
| collection | DOAJ |
| description | Maize is one of the major crops in the world; however, diseases caused by various pathogens seriously affect its yield and quality. The maize Rp1-D21 mutant (mt) caused by the intragenic recombination between two nucleotide-binding, leucine-rich repeat (NLR) proteins, exhibits autoactive hypersensitive response (HR). In this study, we integrated transcriptomic and metabolomic analyses to identify differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) in Rp1-D21 mt compared to the wild type (WT). Genes involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) were enriched among the DEGs. The salicylic acid (SA) pathway and the phenylpropanoid biosynthesis pathway were induced at both the transcriptional and metabolic levels. The DAMs identified included lipids, flavones, and phenolic acids, including 2,5-DHBA O-hexoside, the production of which is catalyzed by uridinediphosphate (UDP)-dependent glycosyltransferase (UGT). Four maize UGTs (ZmUGTs) homologous genes were among the DEGs. Functional analysis by transient co-expression in Nicotiana benthamiana showed that ZmUGT9250 and ZmUGT5174, but not ZmUGT9256 and ZmUGT8707, partially suppressed the HR triggered by Rp1-D21 or its N-terminal coiled-coil signaling domain (CCD21). None of the four ZmUGTs interacted physically with CCD21 in yeast two-hybrid or co-immunoprecipitation assays. We discuss the possibility that ZmUGTs might be involved in defense response by regulating SA homeostasis. |
| first_indexed | 2024-12-16T20:46:15Z |
| format | Article |
| id | doaj.art-e2f203264ce24b0b89d2ea9ae60785e6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T20:46:15Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e2f203264ce24b0b89d2ea9ae60785e62022-12-21T22:16:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-09-011210.3389/fpls.2021.738261738261Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense ResponseChunxia Ge0Chunxia Ge1Yi-Ge Wang2Shouping Lu3Xiang Yu Zhao4Bing-Kai Hou5Peter J. Balint-Kurti6Peter J. Balint-Kurti7Guan-Feng Wang8The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaSchool of Public Health and Management, Binzhou Medical University, Yantai, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaMaize Research Institute, Shandong Academy of Agricultural Sciences, Jinan, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaDepartment of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United StatesUS Department of Agriculture-Agricultural Research Service, Plant Science Research Unit, Raleigh, NC, United StatesThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, ChinaMaize is one of the major crops in the world; however, diseases caused by various pathogens seriously affect its yield and quality. The maize Rp1-D21 mutant (mt) caused by the intragenic recombination between two nucleotide-binding, leucine-rich repeat (NLR) proteins, exhibits autoactive hypersensitive response (HR). In this study, we integrated transcriptomic and metabolomic analyses to identify differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) in Rp1-D21 mt compared to the wild type (WT). Genes involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) were enriched among the DEGs. The salicylic acid (SA) pathway and the phenylpropanoid biosynthesis pathway were induced at both the transcriptional and metabolic levels. The DAMs identified included lipids, flavones, and phenolic acids, including 2,5-DHBA O-hexoside, the production of which is catalyzed by uridinediphosphate (UDP)-dependent glycosyltransferase (UGT). Four maize UGTs (ZmUGTs) homologous genes were among the DEGs. Functional analysis by transient co-expression in Nicotiana benthamiana showed that ZmUGT9250 and ZmUGT5174, but not ZmUGT9256 and ZmUGT8707, partially suppressed the HR triggered by Rp1-D21 or its N-terminal coiled-coil signaling domain (CCD21). None of the four ZmUGTs interacted physically with CCD21 in yeast two-hybrid or co-immunoprecipitation assays. We discuss the possibility that ZmUGTs might be involved in defense response by regulating SA homeostasis.https://www.frontiersin.org/articles/10.3389/fpls.2021.738261/fulldisease resistanceETIhypersensitive responsemaizeNLRsalicylic acid |
| spellingShingle | Chunxia Ge Chunxia Ge Yi-Ge Wang Shouping Lu Xiang Yu Zhao Bing-Kai Hou Peter J. Balint-Kurti Peter J. Balint-Kurti Guan-Feng Wang Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense Response Frontiers in Plant Science disease resistance ETI hypersensitive response maize NLR salicylic acid |
| title | Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense Response |
| title_full | Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense Response |
| title_fullStr | Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense Response |
| title_full_unstemmed | Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense Response |
| title_short | Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense Response |
| title_sort | multi omics analyses reveal the regulatory network and the function of zmugts in maize defense response |
| topic | disease resistance ETI hypersensitive response maize NLR salicylic acid |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.738261/full |
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