Biodiesel production from alternative raw materials using a heterogeneous low ordered biosilicified enzyme as biocatalyst
Abstract Background Cumulative reported evidence has indicated that renewable feedstocks are a promising alternative source to fossil platforms for the production of fuels and chemicals. In that regard, the development of new, highly active, selective, and easy to recover and reuse catalysts for bio...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-03-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-021-01917-x |
| _version_ | 1817977826994814976 |
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| author | Gabriel Orlando Ferrero Edgar Maximiliano Sánchez Faba Griselda Alejandra Eimer |
| author_facet | Gabriel Orlando Ferrero Edgar Maximiliano Sánchez Faba Griselda Alejandra Eimer |
| author_sort | Gabriel Orlando Ferrero |
| collection | DOAJ |
| description | Abstract Background Cumulative reported evidence has indicated that renewable feedstocks are a promising alternative source to fossil platforms for the production of fuels and chemicals. In that regard, the development of new, highly active, selective, and easy to recover and reuse catalysts for biomass conversions is urgently needed. The combination of enzymatic and inorganic heterogeneous catalysis generates an unprecedented platform that combines the advantages of both, the catalytic efficiency and selectivity of enzymes with the ordered structure, high porosity, mechanical, thermal and chemical resistance of mesoporous materials to obtain enzymatic heterogeneous catalysts. Enzymatic mineralization with an organic silicon precursor (biosilicification) is a promising and emerging approach for the generation of solid hybrid biocatalysts with exceptional stability under severe use conditions. Herein, we assessed the putative advantages of the biosilicification technology for developing an improved efficient and stable biocatalyst for sustainable biofuel production. Results A series of solid enzymatic catalysts denominated LOBE (low ordered biosilicified enzyme) were synthesized from Pseudomonas fluorescens lipase and tetraethyl orthosilicate. The microscopic structure and physicochemical properties characterization revealed that the enzyme formed aggregates that were contained in the heart of silicon-covered micelles, providing active sites with the ability to process different raw materials (commercial sunflower and soybean oils, Jatropha excisa oil, waste frying oil, acid oil from soybean soapstock, and pork fat) to produce first- and second-generation biodiesel. Ester content ranged from 81 to 93% wt depending on the raw material used for biodiesel synthesis. Conclusions A heterogeneous enzymatic biocatalyst, LOBE4, for efficient biodiesel production was successfully developed in a single-step synthesis reaction using biosilicification technology. LOBE4 showed to be highly efficient in converting refined, non-edible and residual oils (with high water and free fatty acid contents) and ethanol into biodiesel. Thus, LOBE4 emerges as a promising tool to produce second-generation biofuels, with significant implications for establishing a circular economy and reducing the carbon footprint. |
| first_indexed | 2024-04-13T22:20:27Z |
| format | Article |
| id | doaj.art-e2f2688e660a4dd9af29fd81d122c9c3 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T22:20:27Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-e2f2688e660a4dd9af29fd81d122c9c32022-12-22T02:27:15ZengBMCBiotechnology for Biofuels1754-68342021-03-0114111110.1186/s13068-021-01917-xBiodiesel production from alternative raw materials using a heterogeneous low ordered biosilicified enzyme as biocatalystGabriel Orlando Ferrero0Edgar Maximiliano Sánchez Faba1Griselda Alejandra Eimer2CITeQ-UTN-CONICET, Facultad Regional Córdoba, Universidad Tecnológica NacionalCITeQ-UTN-CONICET, Facultad Regional Córdoba, Universidad Tecnológica NacionalCITeQ-UTN-CONICET, Facultad Regional Córdoba, Universidad Tecnológica NacionalAbstract Background Cumulative reported evidence has indicated that renewable feedstocks are a promising alternative source to fossil platforms for the production of fuels and chemicals. In that regard, the development of new, highly active, selective, and easy to recover and reuse catalysts for biomass conversions is urgently needed. The combination of enzymatic and inorganic heterogeneous catalysis generates an unprecedented platform that combines the advantages of both, the catalytic efficiency and selectivity of enzymes with the ordered structure, high porosity, mechanical, thermal and chemical resistance of mesoporous materials to obtain enzymatic heterogeneous catalysts. Enzymatic mineralization with an organic silicon precursor (biosilicification) is a promising and emerging approach for the generation of solid hybrid biocatalysts with exceptional stability under severe use conditions. Herein, we assessed the putative advantages of the biosilicification technology for developing an improved efficient and stable biocatalyst for sustainable biofuel production. Results A series of solid enzymatic catalysts denominated LOBE (low ordered biosilicified enzyme) were synthesized from Pseudomonas fluorescens lipase and tetraethyl orthosilicate. The microscopic structure and physicochemical properties characterization revealed that the enzyme formed aggregates that were contained in the heart of silicon-covered micelles, providing active sites with the ability to process different raw materials (commercial sunflower and soybean oils, Jatropha excisa oil, waste frying oil, acid oil from soybean soapstock, and pork fat) to produce first- and second-generation biodiesel. Ester content ranged from 81 to 93% wt depending on the raw material used for biodiesel synthesis. Conclusions A heterogeneous enzymatic biocatalyst, LOBE4, for efficient biodiesel production was successfully developed in a single-step synthesis reaction using biosilicification technology. LOBE4 showed to be highly efficient in converting refined, non-edible and residual oils (with high water and free fatty acid contents) and ethanol into biodiesel. Thus, LOBE4 emerges as a promising tool to produce second-generation biofuels, with significant implications for establishing a circular economy and reducing the carbon footprint.https://doi.org/10.1186/s13068-021-01917-xEnzymatic biosilicificationSecond-generation biodieselAlternative oilsMesoporous materialPseudomonas fluorescens lipase |
| spellingShingle | Gabriel Orlando Ferrero Edgar Maximiliano Sánchez Faba Griselda Alejandra Eimer Biodiesel production from alternative raw materials using a heterogeneous low ordered biosilicified enzyme as biocatalyst Biotechnology for Biofuels Enzymatic biosilicification Second-generation biodiesel Alternative oils Mesoporous material Pseudomonas fluorescens lipase |
| title | Biodiesel production from alternative raw materials using a heterogeneous low ordered biosilicified enzyme as biocatalyst |
| title_full | Biodiesel production from alternative raw materials using a heterogeneous low ordered biosilicified enzyme as biocatalyst |
| title_fullStr | Biodiesel production from alternative raw materials using a heterogeneous low ordered biosilicified enzyme as biocatalyst |
| title_full_unstemmed | Biodiesel production from alternative raw materials using a heterogeneous low ordered biosilicified enzyme as biocatalyst |
| title_short | Biodiesel production from alternative raw materials using a heterogeneous low ordered biosilicified enzyme as biocatalyst |
| title_sort | biodiesel production from alternative raw materials using a heterogeneous low ordered biosilicified enzyme as biocatalyst |
| topic | Enzymatic biosilicification Second-generation biodiesel Alternative oils Mesoporous material Pseudomonas fluorescens lipase |
| url | https://doi.org/10.1186/s13068-021-01917-x |
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