Evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico-vaginal pap smears
Background and Aim: The natural history of cervical cancer is unique that it is preceded by a precancerous condition for a long time. Morphometry as a tool can be used in early and accurate diagnosis of these precancerous and cancerous lesions. The present study aims at assessing the utility of cell...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Journal of Cytology |
| Subjects: | |
| Online Access: | http://www.jcytol.org/article.asp?issn=0970-9371;year=2023;volume=40;issue=1;spage=5;epage=11;aulast=Mishra |
| _version_ | 1797824207576367104 |
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| author | Satyam Mishra Ujjawal Khurana Neelkamal Kapoor Ankur Joshi Deepti Joshi |
| author_facet | Satyam Mishra Ujjawal Khurana Neelkamal Kapoor Ankur Joshi Deepti Joshi |
| author_sort | Satyam Mishra |
| collection | DOAJ |
| description | Background and Aim: The natural history of cervical cancer is unique that it is preceded by a precancerous condition for a long time. Morphometry as a tool can be used in early and accurate diagnosis of these precancerous and cancerous lesions. The present study aims at assessing the utility of cellular and nuclear morphometry in differentiating squamous cell abnormality from benign conditions and also differentiating the categories of squamous cell abnormalities. Materials and Methods: Forty-eight diagnosed cases of squamous epithelial cell abnormality, that is, 10 cases each of atypical squamous cell of undetermined significance ( ASC-US), low-grade squamous intraepithelial lesion (LSIL), high-grade SIL (HSIL), and squamous cell carcinoma (SCC) and eight cases of ASC-H (ASC cannot exclude HSIL), were made the sample population and compared with a control population of 10 cases of negative for intraepithelial lesion or malignancy (NILM). Parameters like nuclear area (NA), nuclear perimeter (NP), nuclear diameter (ND), nuclear compactness (NC), cellular area (CA), cellular diameter (CD), cellular perimeter (CP), and nucleocytoplasmic (N/C) ratio were used. Results: There was a significant difference in the six groups of squamous cell abnormality based on NA, NP, ND, CA, CP, and CD (P < 0.05) using one-way analysis of variance. Nuclear morphometry parameters like NA, NP, and ND were found to be the maximum for HSIL, followed by LSIL, ASC-H, ASC-US, SCC, and NILM groups in decreasing order. The mean CA, CP, and CD were found to be the maximum for NILM, followed by LSIL, ASC-US, HSIL, ASC-H, and SCC in decreasing order. On post hoc analysis, the lesions can be divided into three groups: NILM/normal; ASC-US and LSIL; and ASC-H, HSIL, and SCC, based on N/C ratio. Conclusion: In cervical lesions, holistic parameter of cytonucleomorphometry should be taken rather than taking nuclear morphometry only. N/C ratio is a highly statistically significant parameter that can differentiate between low-grade lesions and high-grade lesions. |
| first_indexed | 2024-03-13T10:35:32Z |
| format | Article |
| id | doaj.art-e2f84cff625b4d3fb133dff5845a0f61 |
| institution | Directory Open Access Journal |
| issn | 0970-9371 |
| language | English |
| last_indexed | 2024-03-13T10:35:32Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Cytology |
| spelling | doaj.art-e2f84cff625b4d3fb133dff5845a0f612023-05-18T06:54:58ZengWolters Kluwer Medknow PublicationsJournal of Cytology0970-93712023-01-0140151110.4103/joc.joc_73_22Evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico-vaginal pap smearsSatyam MishraUjjawal KhuranaNeelkamal KapoorAnkur JoshiDeepti JoshiBackground and Aim: The natural history of cervical cancer is unique that it is preceded by a precancerous condition for a long time. Morphometry as a tool can be used in early and accurate diagnosis of these precancerous and cancerous lesions. The present study aims at assessing the utility of cellular and nuclear morphometry in differentiating squamous cell abnormality from benign conditions and also differentiating the categories of squamous cell abnormalities. Materials and Methods: Forty-eight diagnosed cases of squamous epithelial cell abnormality, that is, 10 cases each of atypical squamous cell of undetermined significance ( ASC-US), low-grade squamous intraepithelial lesion (LSIL), high-grade SIL (HSIL), and squamous cell carcinoma (SCC) and eight cases of ASC-H (ASC cannot exclude HSIL), were made the sample population and compared with a control population of 10 cases of negative for intraepithelial lesion or malignancy (NILM). Parameters like nuclear area (NA), nuclear perimeter (NP), nuclear diameter (ND), nuclear compactness (NC), cellular area (CA), cellular diameter (CD), cellular perimeter (CP), and nucleocytoplasmic (N/C) ratio were used. Results: There was a significant difference in the six groups of squamous cell abnormality based on NA, NP, ND, CA, CP, and CD (P < 0.05) using one-way analysis of variance. Nuclear morphometry parameters like NA, NP, and ND were found to be the maximum for HSIL, followed by LSIL, ASC-H, ASC-US, SCC, and NILM groups in decreasing order. The mean CA, CP, and CD were found to be the maximum for NILM, followed by LSIL, ASC-US, HSIL, ASC-H, and SCC in decreasing order. On post hoc analysis, the lesions can be divided into three groups: NILM/normal; ASC-US and LSIL; and ASC-H, HSIL, and SCC, based on N/C ratio. Conclusion: In cervical lesions, holistic parameter of cytonucleomorphometry should be taken rather than taking nuclear morphometry only. N/C ratio is a highly statistically significant parameter that can differentiate between low-grade lesions and high-grade lesions.http://www.jcytol.org/article.asp?issn=0970-9371;year=2023;volume=40;issue=1;spage=5;epage=11;aulast=Mishracarcinoma cervixcervical cancer screeningcytonucleomorphometrymorphometrysquamous intraepithelial lesionthe bethesda system |
| spellingShingle | Satyam Mishra Ujjawal Khurana Neelkamal Kapoor Ankur Joshi Deepti Joshi Evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico-vaginal pap smears Journal of Cytology carcinoma cervix cervical cancer screening cytonucleomorphometry morphometry squamous intraepithelial lesion the bethesda system |
| title | Evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico-vaginal pap smears |
| title_full | Evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico-vaginal pap smears |
| title_fullStr | Evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico-vaginal pap smears |
| title_full_unstemmed | Evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico-vaginal pap smears |
| title_short | Evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico-vaginal pap smears |
| title_sort | evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico vaginal pap smears |
| topic | carcinoma cervix cervical cancer screening cytonucleomorphometry morphometry squamous intraepithelial lesion the bethesda system |
| url | http://www.jcytol.org/article.asp?issn=0970-9371;year=2023;volume=40;issue=1;spage=5;epage=11;aulast=Mishra |
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