Modulation of Nrf2 by quercetin in doxorubicin-treated rats
Doxorubicin (DOXO), a potent and widely used chemotherapeutic agent, causes irreversible heart failure by increasing oxidative stress, which limits its clinical utility. Nuclear factor erythroid-derived 2 -like 2 (Nrf2) is a prominent central regulator of cellular impenetrable to oxidants. The purpo...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020306484 |
| _version_ | 1811337420502728704 |
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| author | Anish Sharma Mihir Parikh Hital Shah Tejal Gandhi |
| author_facet | Anish Sharma Mihir Parikh Hital Shah Tejal Gandhi |
| author_sort | Anish Sharma |
| collection | DOAJ |
| description | Doxorubicin (DOXO), a potent and widely used chemotherapeutic agent, causes irreversible heart failure by increasing oxidative stress, which limits its clinical utility. Nuclear factor erythroid-derived 2 -like 2 (Nrf2) is a prominent central regulator of cellular impenetrable to oxidants. The purpose of the study is to assess the ameliorative outcome of quercetin in cardiomyopathic rats induced by doxorubicin. Cardiomyopathy was produced in rats by single intraperitoneal weekly with DOXO (2 mg/kg) for 4 weeks. The rats were divided into five groups: (I) control group; (II) DOXO (2 mg/kg, i.p.) group; (III–V) DOXO + quercetin (10 mg/kg, 25 mg/kg and 50 mg/kg, orally), and were treated for 7 weeks. At the end of the treatment duration, cardiac function and biochemical parameters were assessed. Quercetin (10 mg/kg, 25 mg/kg and 50 mg/kg, orally) treatment reduced the raised blood pressure (BP) and left ventricular dysfunction. Withal, it prevented the rise in CKMB and LDH, suggesting the effect of quercetin in the maintaining the integrity of the cell membrane Besides, it also prevented the alteration in electrolyte levels, the activity of ATPase, and antioxidant status. Quercetin increased Nrf2 mRNA expression and reduced histological abnormalities compared 11 Anish Sharma et al., MODULATION OF NRF2 BY QUERCETIN IN DOXORUBICIN TREATED RATS.to the DOXO control group. In conclusion, quercetin protected against DOXO- induced cardiomyopathy, by increasing expression of NRF2, and thereby increasing antioxidant defense and restoring biochemical and histological abnormalities. |
| first_indexed | 2024-04-13T17:54:38Z |
| format | Article |
| id | doaj.art-e2f92b1babf14a0e843187a24942a623 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-13T17:54:38Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-e2f92b1babf14a0e843187a24942a6232022-12-22T02:36:33ZengElsevierHeliyon2405-84402020-04-0164e03803Modulation of Nrf2 by quercetin in doxorubicin-treated ratsAnish Sharma0Mihir Parikh1Hital Shah2Tejal Gandhi3Corresponding author.; Pharmacology Department, Anand Pharmacy College, Anand, 388001, Gujarat, IndiaPharmacology Department, Anand Pharmacy College, Anand, 388001, Gujarat, IndiaPharmacology Department, Anand Pharmacy College, Anand, 388001, Gujarat, IndiaPharmacology Department, Anand Pharmacy College, Anand, 388001, Gujarat, IndiaDoxorubicin (DOXO), a potent and widely used chemotherapeutic agent, causes irreversible heart failure by increasing oxidative stress, which limits its clinical utility. Nuclear factor erythroid-derived 2 -like 2 (Nrf2) is a prominent central regulator of cellular impenetrable to oxidants. The purpose of the study is to assess the ameliorative outcome of quercetin in cardiomyopathic rats induced by doxorubicin. Cardiomyopathy was produced in rats by single intraperitoneal weekly with DOXO (2 mg/kg) for 4 weeks. The rats were divided into five groups: (I) control group; (II) DOXO (2 mg/kg, i.p.) group; (III–V) DOXO + quercetin (10 mg/kg, 25 mg/kg and 50 mg/kg, orally), and were treated for 7 weeks. At the end of the treatment duration, cardiac function and biochemical parameters were assessed. Quercetin (10 mg/kg, 25 mg/kg and 50 mg/kg, orally) treatment reduced the raised blood pressure (BP) and left ventricular dysfunction. Withal, it prevented the rise in CKMB and LDH, suggesting the effect of quercetin in the maintaining the integrity of the cell membrane Besides, it also prevented the alteration in electrolyte levels, the activity of ATPase, and antioxidant status. Quercetin increased Nrf2 mRNA expression and reduced histological abnormalities compared 11 Anish Sharma et al., MODULATION OF NRF2 BY QUERCETIN IN DOXORUBICIN TREATED RATS.to the DOXO control group. In conclusion, quercetin protected against DOXO- induced cardiomyopathy, by increasing expression of NRF2, and thereby increasing antioxidant defense and restoring biochemical and histological abnormalities.http://www.sciencedirect.com/science/article/pii/S2405844020306484DoxorubicinCardiomyopathyQuercetinNRF2SD ratsAntioxidant parameters |
| spellingShingle | Anish Sharma Mihir Parikh Hital Shah Tejal Gandhi Modulation of Nrf2 by quercetin in doxorubicin-treated rats Heliyon Doxorubicin Cardiomyopathy Quercetin NRF2 SD rats Antioxidant parameters |
| title | Modulation of Nrf2 by quercetin in doxorubicin-treated rats |
| title_full | Modulation of Nrf2 by quercetin in doxorubicin-treated rats |
| title_fullStr | Modulation of Nrf2 by quercetin in doxorubicin-treated rats |
| title_full_unstemmed | Modulation of Nrf2 by quercetin in doxorubicin-treated rats |
| title_short | Modulation of Nrf2 by quercetin in doxorubicin-treated rats |
| title_sort | modulation of nrf2 by quercetin in doxorubicin treated rats |
| topic | Doxorubicin Cardiomyopathy Quercetin NRF2 SD rats Antioxidant parameters |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020306484 |
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