Postbiotic potential of Bacillus velezensis KMU01 cell-free supernatant for the alleviation of obesity in mice
Attention toward the preventive effects of postbiotics on metabolic diseases has increased because of greater stability and safety over probiotics. However, studies regarding the bioactive effects of postbiotics, especially from probiotic Bacillus strains, are relatively limited. The anti-obesity ef...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024012945 |
| _version_ | 1797259968924090368 |
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| author | Hee Hyun Shin Jong-Hoon Kim Ye-Jin Jung Mi-Sun Kwak Moon-Hee Sung Jee-Young Imm |
| author_facet | Hee Hyun Shin Jong-Hoon Kim Ye-Jin Jung Mi-Sun Kwak Moon-Hee Sung Jee-Young Imm |
| author_sort | Hee Hyun Shin |
| collection | DOAJ |
| description | Attention toward the preventive effects of postbiotics on metabolic diseases has increased because of greater stability and safety over probiotics. However, studies regarding the bioactive effects of postbiotics, especially from probiotic Bacillus strains, are relatively limited. The anti-obesity effects of the cell-free culture supernatant of Bacillus velezensis KMU01 (CFS–B.vele) were evaluated using high-fat-diet (HFD)-induced mice. HFD-induced mice (n = 8 per group) received equal volumes of (1) CFS-B.vele (114 mg/kg) in PBS, (2) Xenical in PBS, or (3) PBS alone by oral gavage daily for 13 weeks. The results demonstrated that CFS-B.vele changed the gut microbiota and showed anti-obesity effects in HFD-induced obese mice. The elevated Firmicutes/Bacteroidota ratio induced by HFD was decreased in the CFS-B.vele group compared to the other groups (p < 0.05). The CFS-B.vele intervention led to the enrichment of SCFA-producers, such as Roseburia and Eubacterium, in the cecum, suggesting their potential involvement in the amelioration of obesity. Due to these changes, the various obesity-related biomarkers (body weight, fat in tissue, white adipose tissue weight and size, serum LDL-cholesterol level, hepatic lipid accumulation, and adipogenesis/lipogenesis-related gene/protein expression) were improved. Our findings suggest that CFS-B.vele has potential as a novel anti-obesity agent through modulation of the gut microbiota. |
| first_indexed | 2024-04-24T23:17:52Z |
| format | Article |
| id | doaj.art-e2f98812508044e59b769632b3d68e62 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:17:52Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-e2f98812508044e59b769632b3d68e622024-03-17T07:55:01ZengElsevierHeliyon2405-84402024-03-01105e25263Postbiotic potential of Bacillus velezensis KMU01 cell-free supernatant for the alleviation of obesity in miceHee Hyun Shin0Jong-Hoon Kim1Ye-Jin Jung2Mi-Sun Kwak3Moon-Hee Sung4Jee-Young Imm5Department of Foods and Nutrition, Kookmin University, Seoul, 02707, South KoreaKookminBio Corporation, Seoul, 02826, South KoreaKookminBio Corporation, Seoul, 02826, South KoreaKookminBio Corporation, Seoul, 02826, South KoreaKookminBio Corporation, Seoul, 02826, South KoreaDepartment of Foods and Nutrition, Kookmin University, Seoul, 02707, South Korea; Corresponding author.Attention toward the preventive effects of postbiotics on metabolic diseases has increased because of greater stability and safety over probiotics. However, studies regarding the bioactive effects of postbiotics, especially from probiotic Bacillus strains, are relatively limited. The anti-obesity effects of the cell-free culture supernatant of Bacillus velezensis KMU01 (CFS–B.vele) were evaluated using high-fat-diet (HFD)-induced mice. HFD-induced mice (n = 8 per group) received equal volumes of (1) CFS-B.vele (114 mg/kg) in PBS, (2) Xenical in PBS, or (3) PBS alone by oral gavage daily for 13 weeks. The results demonstrated that CFS-B.vele changed the gut microbiota and showed anti-obesity effects in HFD-induced obese mice. The elevated Firmicutes/Bacteroidota ratio induced by HFD was decreased in the CFS-B.vele group compared to the other groups (p < 0.05). The CFS-B.vele intervention led to the enrichment of SCFA-producers, such as Roseburia and Eubacterium, in the cecum, suggesting their potential involvement in the amelioration of obesity. Due to these changes, the various obesity-related biomarkers (body weight, fat in tissue, white adipose tissue weight and size, serum LDL-cholesterol level, hepatic lipid accumulation, and adipogenesis/lipogenesis-related gene/protein expression) were improved. Our findings suggest that CFS-B.vele has potential as a novel anti-obesity agent through modulation of the gut microbiota.http://www.sciencedirect.com/science/article/pii/S2405844024012945ObesityBacillus velezensis KMU01Cell-free supernatantGut microbiome |
| spellingShingle | Hee Hyun Shin Jong-Hoon Kim Ye-Jin Jung Mi-Sun Kwak Moon-Hee Sung Jee-Young Imm Postbiotic potential of Bacillus velezensis KMU01 cell-free supernatant for the alleviation of obesity in mice Heliyon Obesity Bacillus velezensis KMU01 Cell-free supernatant Gut microbiome |
| title | Postbiotic potential of Bacillus velezensis KMU01 cell-free supernatant for the alleviation of obesity in mice |
| title_full | Postbiotic potential of Bacillus velezensis KMU01 cell-free supernatant for the alleviation of obesity in mice |
| title_fullStr | Postbiotic potential of Bacillus velezensis KMU01 cell-free supernatant for the alleviation of obesity in mice |
| title_full_unstemmed | Postbiotic potential of Bacillus velezensis KMU01 cell-free supernatant for the alleviation of obesity in mice |
| title_short | Postbiotic potential of Bacillus velezensis KMU01 cell-free supernatant for the alleviation of obesity in mice |
| title_sort | postbiotic potential of bacillus velezensis kmu01 cell free supernatant for the alleviation of obesity in mice |
| topic | Obesity Bacillus velezensis KMU01 Cell-free supernatant Gut microbiome |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024012945 |
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