Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney
The aim of this study was to evaluate KCNQ1 K+ channel expression in the frog kidney of Rana esculenta. KCNQ1 K+ channel, also known as KvLQT1, is the pore forming a-subunit of the IKs K+ channel, a delayed rectifier voltage-gated K+ channel, which has an important role in water and salt transport i...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2010-02-01
|
| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/1672 |
| _version_ | 1811331305853419520 |
|---|---|
| author | S. Ćirović J. Marković-Lipkovski J. Todorović J. Nešović-Ostojić M. Jović S. Ilić S. Tatić D. Čemerikić |
| author_facet | S. Ćirović J. Marković-Lipkovski J. Todorović J. Nešović-Ostojić M. Jović S. Ilić S. Tatić D. Čemerikić |
| author_sort | S. Ćirović |
| collection | DOAJ |
| description | The aim of this study was to evaluate KCNQ1 K+ channel expression in the frog kidney of Rana esculenta. KCNQ1 K+ channel, also known as KvLQT1, is the pore forming a-subunit of the IKs K+ channel, a delayed rectifier voltage-gated K+ channel, which has an important role in water and salt transport in the kidney and gastrointestinal tract. The expression of KCNQ1 K+ channel along tubular epithelium differs from species to species. In the present study the expression of KCNQ1 K+ channel in the frog kidney has been demonstrated by immunohistochemistry. The presence of KCNQ1 K+ channel was demonstrated in the epithelial cells of distal convoluted tubule and collecting duct. However, the pattern of expression of KCNQ1 K+ channel differs between distal convoluted tubules and collecting duct. All epithelial cells of distal convoluted tubules revealed basolateral expression of KCNQ1 K+ channel. On the contrary, only the single cells of collecting duct, probably intercalated cells, showed diffuse cell surface staining with antibodies against KCNQ1 K+ channel. These findings suggest that KCNQ1 K+ channel has cell-specific roles in renal potassium ion transport. |
| first_indexed | 2024-04-13T16:17:37Z |
| format | Article |
| id | doaj.art-e2fc02f848044336bb73ac5e23835278 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-13T16:17:37Z |
| publishDate | 2010-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-e2fc02f848044336bb73ac5e238352782022-12-22T02:40:01ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062010-02-01541e7e710.4081/ejh.2010.e71099Differential expression of KCNQ1 K+ channel in tubular cells of frog kidneyS. Ćirović0J. Marković-Lipkovski1J. Todorović2J. Nešović-Ostojić3M. Jović4S. Ilić5S. Tatić6D. Čemerikić7University of BelgradeUniversity of BelgradeUniversity of BelgradeUniversity of BelgradeMilitary Medical Academy, BelgradeMilitary Medical Academy, BelgradeUniversity of BelgradeUniversity of BelgradeThe aim of this study was to evaluate KCNQ1 K+ channel expression in the frog kidney of Rana esculenta. KCNQ1 K+ channel, also known as KvLQT1, is the pore forming a-subunit of the IKs K+ channel, a delayed rectifier voltage-gated K+ channel, which has an important role in water and salt transport in the kidney and gastrointestinal tract. The expression of KCNQ1 K+ channel along tubular epithelium differs from species to species. In the present study the expression of KCNQ1 K+ channel in the frog kidney has been demonstrated by immunohistochemistry. The presence of KCNQ1 K+ channel was demonstrated in the epithelial cells of distal convoluted tubule and collecting duct. However, the pattern of expression of KCNQ1 K+ channel differs between distal convoluted tubules and collecting duct. All epithelial cells of distal convoluted tubules revealed basolateral expression of KCNQ1 K+ channel. On the contrary, only the single cells of collecting duct, probably intercalated cells, showed diffuse cell surface staining with antibodies against KCNQ1 K+ channel. These findings suggest that KCNQ1 K+ channel has cell-specific roles in renal potassium ion transport.http://www.ejh.it/index.php/ejh/article/view/1672KCNQ1 K+ channelRana esculentafrog kidneyimmunohistochemistry |
| spellingShingle | S. Ćirović J. Marković-Lipkovski J. Todorović J. Nešović-Ostojić M. Jović S. Ilić S. Tatić D. Čemerikić Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney European Journal of Histochemistry KCNQ1 K+ channel Rana esculenta frog kidney immunohistochemistry |
| title | Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney |
| title_full | Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney |
| title_fullStr | Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney |
| title_full_unstemmed | Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney |
| title_short | Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney |
| title_sort | differential expression of kcnq1 k channel in tubular cells of frog kidney |
| topic | KCNQ1 K+ channel Rana esculenta frog kidney immunohistochemistry |
| url | http://www.ejh.it/index.php/ejh/article/view/1672 |
| work_keys_str_mv | AT scirovic differentialexpressionofkcnq1kchannelintubularcellsoffrogkidney AT jmarkoviclipkovski differentialexpressionofkcnq1kchannelintubularcellsoffrogkidney AT jtodorovic differentialexpressionofkcnq1kchannelintubularcellsoffrogkidney AT jnesovicostojic differentialexpressionofkcnq1kchannelintubularcellsoffrogkidney AT mjovic differentialexpressionofkcnq1kchannelintubularcellsoffrogkidney AT silic differentialexpressionofkcnq1kchannelintubularcellsoffrogkidney AT static differentialexpressionofkcnq1kchannelintubularcellsoffrogkidney AT dcemerikic differentialexpressionofkcnq1kchannelintubularcellsoffrogkidney |