The expression profiling of serum miR-92a, miR-375, and miR-760 in colorectal cancer: An Egyptian study
Dysregulation in microRNA expression is a common feature in colorectal cancer. Due to the inconsistent results regarding serum miR-92a expression pattern and the insufficient studies on serum miR-375 and miR-760, we aimed in this study to investigate their expression profile and diagnostic and progn...
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| Format: | Article |
| Language: | English |
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IOS Press
2017-05-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317705765 |
| _version_ | 1819121711337963520 |
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| author | Ahmed Elshafei Olfat Shaker Ossama Abd El-motaal Tarek Salman |
| author_facet | Ahmed Elshafei Olfat Shaker Ossama Abd El-motaal Tarek Salman |
| author_sort | Ahmed Elshafei |
| collection | DOAJ |
| description | Dysregulation in microRNA expression is a common feature in colorectal cancer. Due to the inconsistent results regarding serum miR-92a expression pattern and the insufficient studies on serum miR-375 and miR-760, we aimed in this study to investigate their expression profile and diagnostic and prognostic power in Egyptian colorectal cancer patients. The expression profile of miR-92a, miR-375, and miR-760 was determined in the sera of 64 colorectal cancer patients using quantitative real-time reverse transcription polymerase chain reaction in comparison to 27 healthy control subjects. The expression fold change of the studied microRNAs was correlated with patients’ clinicopathological features. Receiver operating characteristic curve analysis was done to determine the role of these microRNAs in colorectal cancer diagnosis and follow-up according to the yielded area under the curve. The expression pattern of miR-92a was significantly upregulated (3.38 ± 2.52, p < 0.0001), while both of miR-375 and 760 were significantly downregulated (−1.250 ± 1.80, p < 0.0001; −1.710 ± 1.88, p < 0.0001, respectively) in colorectal cancer than the control. MiR-92a was positively correlated ( r = 0.671, p = 0.0001), while miR-375 and miR-760 were inversely correlated ( r = −0.414, p = 0.001; r = −0.644, p = 0.0001) with advanced colorectal cancer stages. Receiver operating characteristic curve analysis disclosed the highest diagnostic potential for miR-760 to discriminate colorectal cancer patients and early-stage colorectal cancer from the control (area under the curve = 0.922 and 0.875, respectively), while the highest prognostic potential for discrimination between colorectal cancer stages was for miR-92a. In conclusion, serum level of miR-92a, miR-375, and miR-760 may serve as biomarkers of colorectal cancer in Egyptian patients with high diagnostic power for miR-760 and high prognostic power for miR-92a. |
| first_indexed | 2024-12-22T06:40:54Z |
| format | Article |
| id | doaj.art-e2fc223eaed745a9ae5c040953c518c1 |
| institution | Directory Open Access Journal |
| issn | 1423-0380 |
| language | English |
| last_indexed | 2024-12-22T06:40:54Z |
| publishDate | 2017-05-01 |
| publisher | IOS Press |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj.art-e2fc223eaed745a9ae5c040953c518c12022-12-21T18:35:26ZengIOS PressTumor Biology1423-03802017-05-013910.1177/1010428317705765The expression profiling of serum miR-92a, miR-375, and miR-760 in colorectal cancer: An Egyptian studyAhmed Elshafei0Olfat Shaker1Ossama Abd El-motaal2Tarek Salman3Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Al-Azhar University, Cairo, EgyptDepartment of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, EgyptDepartment of Biochemistry and Molecular Biology, Faculty of Pharmacy, Al-Azhar University, Cairo, EgyptDepartment of Biochemistry and Molecular Biology, Faculty of Pharmacy, Al-Azhar University, Cairo, EgyptDysregulation in microRNA expression is a common feature in colorectal cancer. Due to the inconsistent results regarding serum miR-92a expression pattern and the insufficient studies on serum miR-375 and miR-760, we aimed in this study to investigate their expression profile and diagnostic and prognostic power in Egyptian colorectal cancer patients. The expression profile of miR-92a, miR-375, and miR-760 was determined in the sera of 64 colorectal cancer patients using quantitative real-time reverse transcription polymerase chain reaction in comparison to 27 healthy control subjects. The expression fold change of the studied microRNAs was correlated with patients’ clinicopathological features. Receiver operating characteristic curve analysis was done to determine the role of these microRNAs in colorectal cancer diagnosis and follow-up according to the yielded area under the curve. The expression pattern of miR-92a was significantly upregulated (3.38 ± 2.52, p < 0.0001), while both of miR-375 and 760 were significantly downregulated (−1.250 ± 1.80, p < 0.0001; −1.710 ± 1.88, p < 0.0001, respectively) in colorectal cancer than the control. MiR-92a was positively correlated ( r = 0.671, p = 0.0001), while miR-375 and miR-760 were inversely correlated ( r = −0.414, p = 0.001; r = −0.644, p = 0.0001) with advanced colorectal cancer stages. Receiver operating characteristic curve analysis disclosed the highest diagnostic potential for miR-760 to discriminate colorectal cancer patients and early-stage colorectal cancer from the control (area under the curve = 0.922 and 0.875, respectively), while the highest prognostic potential for discrimination between colorectal cancer stages was for miR-92a. In conclusion, serum level of miR-92a, miR-375, and miR-760 may serve as biomarkers of colorectal cancer in Egyptian patients with high diagnostic power for miR-760 and high prognostic power for miR-92a.https://doi.org/10.1177/1010428317705765 |
| spellingShingle | Ahmed Elshafei Olfat Shaker Ossama Abd El-motaal Tarek Salman The expression profiling of serum miR-92a, miR-375, and miR-760 in colorectal cancer: An Egyptian study Tumor Biology |
| title | The expression profiling of serum miR-92a, miR-375, and miR-760 in colorectal cancer: An Egyptian study |
| title_full | The expression profiling of serum miR-92a, miR-375, and miR-760 in colorectal cancer: An Egyptian study |
| title_fullStr | The expression profiling of serum miR-92a, miR-375, and miR-760 in colorectal cancer: An Egyptian study |
| title_full_unstemmed | The expression profiling of serum miR-92a, miR-375, and miR-760 in colorectal cancer: An Egyptian study |
| title_short | The expression profiling of serum miR-92a, miR-375, and miR-760 in colorectal cancer: An Egyptian study |
| title_sort | expression profiling of serum mir 92a mir 375 and mir 760 in colorectal cancer an egyptian study |
| url | https://doi.org/10.1177/1010428317705765 |
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