Impact of Prebiotics and Synbiotics Administered <i>in ovo</i> on the Immune Response against Experimental Antigens in Chicken Broilers
The effect of the <i>in ovo</i> application of selected prebiotics and synbiotics on the humoral immune response against T-dependent (SRBC) and T-independent (dextran) antigens and delayed-type hypersensitivity (DTH) to phytohemagglutinin was studied. On the 12th day of incubation, 800 e...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/10/4/643 |
| _version_ | 1797571223366926336 |
|---|---|
| author | Tadeusz Stefaniak Jan P. Madej Stanisław Graczyk Maria Siwek Ewa Łukaszewicz Artur Kowalczyk Marcin Sieńczyk Giuseppe Maiorano Marek Bednarczyk |
| author_facet | Tadeusz Stefaniak Jan P. Madej Stanisław Graczyk Maria Siwek Ewa Łukaszewicz Artur Kowalczyk Marcin Sieńczyk Giuseppe Maiorano Marek Bednarczyk |
| author_sort | Tadeusz Stefaniak |
| collection | DOAJ |
| description | The effect of the <i>in ovo</i> application of selected prebiotics and synbiotics on the humoral immune response against T-dependent (SRBC) and T-independent (dextran) antigens and delayed-type hypersensitivity (DTH) to phytohemagglutinin was studied. On the 12th day of incubation, 800 eggs (Ross 308) were divided into five groups and injected into the egg air chamber with prebiotic inulin (Pre1), Bi<sup>2</sup>tos (Pre2), a synbiotic composed of inulin and <i>Lactococcus lactis</i> subsp. <i>lactis</i> IBB SL1 (Syn1), a synbiotic composed of Bi<sup>2</sup>tos and <i>L. lactis</i> subsp. <i>cremoris</i> IBB SC1 (Syn2), and physiological saline (control group; C). The chickens were immunized twice at the 7th and 21st day of life with SRBC and dextran. A DTH test was performed on the 7th, 21st, and 35th day. The application of prebiotics and synbiotics had no significant effect on the humoral immune response. SRBC-immunized <i>in ovo</i> Pre1- and Pre2-treated chickens showed significantly higher serum IgG levels than the control. A significant effect on the DTH reaction was detected on the 7th (Pre1 < C) and 21st (Pre2 > Syn2) day. However; Bi<sup>2</sup>tos may transiently stimulate the cellular immune response on the 21st day. It may be concluded that the application of inulin in an egg air chamber on the 12th day of incubation may stimulate the secondary immune response. The inulin-treated group exhibited a lower mortality rate than the control group. |
| first_indexed | 2024-03-10T20:36:19Z |
| format | Article |
| id | doaj.art-e2fcee8eeae844d8938a203a001de883 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T20:36:19Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-e2fcee8eeae844d8938a203a001de8832023-11-19T21:02:17ZengMDPI AGAnimals2076-26152020-04-0110464310.3390/ani10040643Impact of Prebiotics and Synbiotics Administered <i>in ovo</i> on the Immune Response against Experimental Antigens in Chicken BroilersTadeusz Stefaniak0Jan P. Madej1Stanisław Graczyk2Maria Siwek3Ewa Łukaszewicz4Artur Kowalczyk5Marcin Sieńczyk6Giuseppe Maiorano7Marek Bednarczyk8Wroclaw University of Environmental and Life Sciences, Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, 50-375 Wroclaw, PolandWroclaw University of Environmental and Life Sciences, Division of Histology and Embryology, 50-375 Wroclaw, PolandWroclaw University of Environmental and Life Sciences, Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, 50-375 Wroclaw, PolandUTP University of Science and Technology, Department of Animal Biotechnology and Genetics, 85-796 Bydgoszcz, PolandWrocław University of Environmental and Life Sciences, Institute of Animal Breeding, 51-630 Wrocław, PolandWrocław University of Environmental and Life Sciences, Institute of Animal Breeding, 51-630 Wrocław, PolandWroclaw University of Technology, Division of Medicinal Chemistry & Microbiology, 50-372 Wroclaw, PolandUniversity of Molise, Department of Agricultural, Environmental and Food Sciences, 86100 Campobasso, ItalyUTP University of Science and Technology, Department of Animal Biotechnology and Genetics, 85-796 Bydgoszcz, PolandThe effect of the <i>in ovo</i> application of selected prebiotics and synbiotics on the humoral immune response against T-dependent (SRBC) and T-independent (dextran) antigens and delayed-type hypersensitivity (DTH) to phytohemagglutinin was studied. On the 12th day of incubation, 800 eggs (Ross 308) were divided into five groups and injected into the egg air chamber with prebiotic inulin (Pre1), Bi<sup>2</sup>tos (Pre2), a synbiotic composed of inulin and <i>Lactococcus lactis</i> subsp. <i>lactis</i> IBB SL1 (Syn1), a synbiotic composed of Bi<sup>2</sup>tos and <i>L. lactis</i> subsp. <i>cremoris</i> IBB SC1 (Syn2), and physiological saline (control group; C). The chickens were immunized twice at the 7th and 21st day of life with SRBC and dextran. A DTH test was performed on the 7th, 21st, and 35th day. The application of prebiotics and synbiotics had no significant effect on the humoral immune response. SRBC-immunized <i>in ovo</i> Pre1- and Pre2-treated chickens showed significantly higher serum IgG levels than the control. A significant effect on the DTH reaction was detected on the 7th (Pre1 < C) and 21st (Pre2 > Syn2) day. However; Bi<sup>2</sup>tos may transiently stimulate the cellular immune response on the 21st day. It may be concluded that the application of inulin in an egg air chamber on the 12th day of incubation may stimulate the secondary immune response. The inulin-treated group exhibited a lower mortality rate than the control group.https://www.mdpi.com/2076-2615/10/4/643<i>in ovo</i>delayed-type hypersensitivitySRBCdextranimmunoglobulin |
| spellingShingle | Tadeusz Stefaniak Jan P. Madej Stanisław Graczyk Maria Siwek Ewa Łukaszewicz Artur Kowalczyk Marcin Sieńczyk Giuseppe Maiorano Marek Bednarczyk Impact of Prebiotics and Synbiotics Administered <i>in ovo</i> on the Immune Response against Experimental Antigens in Chicken Broilers Animals <i>in ovo</i> delayed-type hypersensitivity SRBC dextran immunoglobulin |
| title | Impact of Prebiotics and Synbiotics Administered <i>in ovo</i> on the Immune Response against Experimental Antigens in Chicken Broilers |
| title_full | Impact of Prebiotics and Synbiotics Administered <i>in ovo</i> on the Immune Response against Experimental Antigens in Chicken Broilers |
| title_fullStr | Impact of Prebiotics and Synbiotics Administered <i>in ovo</i> on the Immune Response against Experimental Antigens in Chicken Broilers |
| title_full_unstemmed | Impact of Prebiotics and Synbiotics Administered <i>in ovo</i> on the Immune Response against Experimental Antigens in Chicken Broilers |
| title_short | Impact of Prebiotics and Synbiotics Administered <i>in ovo</i> on the Immune Response against Experimental Antigens in Chicken Broilers |
| title_sort | impact of prebiotics and synbiotics administered i in ovo i on the immune response against experimental antigens in chicken broilers |
| topic | <i>in ovo</i> delayed-type hypersensitivity SRBC dextran immunoglobulin |
| url | https://www.mdpi.com/2076-2615/10/4/643 |
| work_keys_str_mv | AT tadeuszstefaniak impactofprebioticsandsynbioticsadministerediinovoiontheimmuneresponseagainstexperimentalantigensinchickenbroilers AT janpmadej impactofprebioticsandsynbioticsadministerediinovoiontheimmuneresponseagainstexperimentalantigensinchickenbroilers AT stanisławgraczyk impactofprebioticsandsynbioticsadministerediinovoiontheimmuneresponseagainstexperimentalantigensinchickenbroilers AT mariasiwek impactofprebioticsandsynbioticsadministerediinovoiontheimmuneresponseagainstexperimentalantigensinchickenbroilers AT ewałukaszewicz impactofprebioticsandsynbioticsadministerediinovoiontheimmuneresponseagainstexperimentalantigensinchickenbroilers AT arturkowalczyk impactofprebioticsandsynbioticsadministerediinovoiontheimmuneresponseagainstexperimentalantigensinchickenbroilers AT marcinsienczyk impactofprebioticsandsynbioticsadministerediinovoiontheimmuneresponseagainstexperimentalantigensinchickenbroilers AT giuseppemaiorano impactofprebioticsandsynbioticsadministerediinovoiontheimmuneresponseagainstexperimentalantigensinchickenbroilers AT marekbednarczyk impactofprebioticsandsynbioticsadministerediinovoiontheimmuneresponseagainstexperimentalantigensinchickenbroilers |