Stellate ganglion block relieves acute lung injury induced by severe acute pancreatitis via the miR-155-5p/SOCS5/JAK2/STAT3 axis
Abstract Acute lung injury (ALI), a prevalent complication of severe acute pancreatitis (SAP), is also a leading contributor to respiratory failure and even death of SAP patients. Here, we intended to investigate the function and mechanism of stellate ganglion block (SGB) in ameliorating SAP-induced...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-022-00860-3 |
| _version_ | 1797984642596339712 |
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| author | Lei Wang Na Yuan Yuanli Li Qinqin Ma Ying Zhou Zhifei Qiao Shutie Li Chunyan Liu Liqian Zhang Meng Yuan Jianjing Sun |
| author_facet | Lei Wang Na Yuan Yuanli Li Qinqin Ma Ying Zhou Zhifei Qiao Shutie Li Chunyan Liu Liqian Zhang Meng Yuan Jianjing Sun |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Abstract Acute lung injury (ALI), a prevalent complication of severe acute pancreatitis (SAP), is also a leading contributor to respiratory failure and even death of SAP patients. Here, we intended to investigate the function and mechanism of stellate ganglion block (SGB) in ameliorating SAP-induced ALI (SAP-ALI). We engineered an SAP-ALI model in rats and treated them with SGB. HE staining and the dry and wet method were implemented to evaluate pathological alterations in the tissues and pulmonary edema. The rats serum changes of the profiles of TNF-α, IL-6, IL-1β, and IL-10 were examined. The profiles of miR-155-5p and SOCS5/JAK2/STAT3 were detected. Functional assays were performed for confirming the role of miR-155-5p in modulating the SOCS5/JAK2/STAT3 pathway in pulmonary epithelial cells. Our findings revealed that SGB vigorously alleviated SAP rat lung tissue damage and lung edema and lessened the generation of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β. SGB enhanced SOCS5 expression, hampered miR-155-5p, and suppressed JAK2/STAT3 pathway activation. As evidenced by mechanism studies, miR-155-5p targeted the 3′UTR of SOCS5 and repressed its expression, hence resulting in JAK2/STAT3 pathway activation. During animal trials, we discovered that SGB ameliorated SAP-ALI, boosted SOCS5 expression, and mitigated the levels of pro-inflammatory factors and miR-155-5p in the plasma. In vitro, miR-155-5p overexpression substantially facilitated pulmonary epithelial cell apoptosis, inflammation, and JAK2/STAT3 pathway activation and restrained SOCS5 expression. All in all, our work hinted that SGB could modulate the miR-155-5p/SOCS5/JAK2/STAT3 axis to alleviate SAP-ALI. |
| first_indexed | 2024-04-11T07:05:02Z |
| format | Article |
| id | doaj.art-e2fddc78265c437bb2ce1666471cf054 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-04-11T07:05:02Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-e2fddc78265c437bb2ce1666471cf0542022-12-22T04:38:25ZengBMCEuropean Journal of Medical Research2047-783X2022-11-0127111410.1186/s40001-022-00860-3Stellate ganglion block relieves acute lung injury induced by severe acute pancreatitis via the miR-155-5p/SOCS5/JAK2/STAT3 axisLei Wang0Na Yuan1Yuanli Li2Qinqin Ma3Ying Zhou4Zhifei Qiao5Shutie Li6Chunyan Liu7Liqian Zhang8Meng Yuan9Jianjing Sun10Department of ICU, The First Affiliated Hospital of Hebei North University, Qiaoxi DistrictDepartment of Radiotherapy, The First Affiliated Hospital of Hebei North UniversityDepartment of ICU, The First Affiliated Hospital of Hebei North University, Qiaoxi DistrictDepartment of ICU, The First Affiliated Hospital of Hebei North University, Qiaoxi DistrictDepartment of ICU, The First Affiliated Hospital of Hebei North University, Qiaoxi DistrictDepartment of ICU, The First Affiliated Hospital of Hebei North University, Qiaoxi DistrictDepartment of ICU, The First Affiliated Hospital of Hebei North University, Qiaoxi DistrictDepartment of ICU, The First Affiliated Hospital of Hebei North University, Qiaoxi DistrictDepartment of ICU, The First Affiliated Hospital of Hebei North University, Qiaoxi DistrictDepartment of Emergency, The First Affiliated Hospital of Hebei North UniversityDepartment of Gastroenterology, The First Affiliated Hospital of Hebei North UniversityAbstract Acute lung injury (ALI), a prevalent complication of severe acute pancreatitis (SAP), is also a leading contributor to respiratory failure and even death of SAP patients. Here, we intended to investigate the function and mechanism of stellate ganglion block (SGB) in ameliorating SAP-induced ALI (SAP-ALI). We engineered an SAP-ALI model in rats and treated them with SGB. HE staining and the dry and wet method were implemented to evaluate pathological alterations in the tissues and pulmonary edema. The rats serum changes of the profiles of TNF-α, IL-6, IL-1β, and IL-10 were examined. The profiles of miR-155-5p and SOCS5/JAK2/STAT3 were detected. Functional assays were performed for confirming the role of miR-155-5p in modulating the SOCS5/JAK2/STAT3 pathway in pulmonary epithelial cells. Our findings revealed that SGB vigorously alleviated SAP rat lung tissue damage and lung edema and lessened the generation of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β. SGB enhanced SOCS5 expression, hampered miR-155-5p, and suppressed JAK2/STAT3 pathway activation. As evidenced by mechanism studies, miR-155-5p targeted the 3′UTR of SOCS5 and repressed its expression, hence resulting in JAK2/STAT3 pathway activation. During animal trials, we discovered that SGB ameliorated SAP-ALI, boosted SOCS5 expression, and mitigated the levels of pro-inflammatory factors and miR-155-5p in the plasma. In vitro, miR-155-5p overexpression substantially facilitated pulmonary epithelial cell apoptosis, inflammation, and JAK2/STAT3 pathway activation and restrained SOCS5 expression. All in all, our work hinted that SGB could modulate the miR-155-5p/SOCS5/JAK2/STAT3 axis to alleviate SAP-ALI.https://doi.org/10.1186/s40001-022-00860-3Stellate ganglion blockAcute lung injurySevere acute pancreatitismiR-155-5pSOCS5 |
| spellingShingle | Lei Wang Na Yuan Yuanli Li Qinqin Ma Ying Zhou Zhifei Qiao Shutie Li Chunyan Liu Liqian Zhang Meng Yuan Jianjing Sun Stellate ganglion block relieves acute lung injury induced by severe acute pancreatitis via the miR-155-5p/SOCS5/JAK2/STAT3 axis European Journal of Medical Research Stellate ganglion block Acute lung injury Severe acute pancreatitis miR-155-5p SOCS5 |
| title | Stellate ganglion block relieves acute lung injury induced by severe acute pancreatitis via the miR-155-5p/SOCS5/JAK2/STAT3 axis |
| title_full | Stellate ganglion block relieves acute lung injury induced by severe acute pancreatitis via the miR-155-5p/SOCS5/JAK2/STAT3 axis |
| title_fullStr | Stellate ganglion block relieves acute lung injury induced by severe acute pancreatitis via the miR-155-5p/SOCS5/JAK2/STAT3 axis |
| title_full_unstemmed | Stellate ganglion block relieves acute lung injury induced by severe acute pancreatitis via the miR-155-5p/SOCS5/JAK2/STAT3 axis |
| title_short | Stellate ganglion block relieves acute lung injury induced by severe acute pancreatitis via the miR-155-5p/SOCS5/JAK2/STAT3 axis |
| title_sort | stellate ganglion block relieves acute lung injury induced by severe acute pancreatitis via the mir 155 5p socs5 jak2 stat3 axis |
| topic | Stellate ganglion block Acute lung injury Severe acute pancreatitis miR-155-5p SOCS5 |
| url | https://doi.org/10.1186/s40001-022-00860-3 |
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