Variable Distribution of DOCK-D Proteins between Cytosol and Nucleoplasm in Cell Lines, Effect of Interleukin-4 on DOCK10 in B-Cell Lymphoid Neoplasms, and Validation of a New DOCK10 Antiserum for Immunofluorescence Studies
Dedicator-of-cytokinesis (DOCK), a family of guanine-nucleotide exchange factors (GEFs), comprises four subfamilies, named from A to D. DOCK-D comprises DOCK9, DOCK10, and DOCK11. The GEF activity involves translocation from the cytoplasm to the plasma membrane (PM), as assessed by the transfection...
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MDPI AG
2021-08-01
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| Series: | Antibodies |
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| Online Access: | https://www.mdpi.com/2073-4468/10/3/33 |
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| author | Natalia Ruiz-Lafuente Alfredo Minguela Jose M. Moraleda Manuel Muro Antonio Parrado |
| author_facet | Natalia Ruiz-Lafuente Alfredo Minguela Jose M. Moraleda Manuel Muro Antonio Parrado |
| author_sort | Natalia Ruiz-Lafuente |
| collection | DOAJ |
| description | Dedicator-of-cytokinesis (DOCK), a family of guanine-nucleotide exchange factors (GEFs), comprises four subfamilies, named from A to D. DOCK-D comprises DOCK9, DOCK10, and DOCK11. The GEF activity involves translocation from the cytoplasm to the plasma membrane (PM), as assessed by the transfection of tagged proteins. However, the cellular localization of endogenous DOCK proteins is poorly understood. In this paper, to gain a better understanding of the role of the DOCK-D proteins, we studied their distribution between cytosol and nucleoplasm in 11 cell lines. DOCK-D proteins were distributed with variable cytosolic or nuclear predominance, although the latter was common for DOCK9 and DOCK11. These results suggest that the DOCK-D proteins may perform new nuclear functions, which remain to be discovered. Furthermore, we found that DOCK10 levels are increased by interleukin-4 (IL-4) in B-cell lymphoid neoplasms other than chronic lymphocytic leukemia (CLL) such as mantle cell lymphoma and diffuse large B-cell lymphoma. We also found evidence for an induction of the cytosolic levels of DOCK10 by IL-4 in CLL. Finally, we obtained a valid DOCK10 antiserum for immunofluorescence (IF) microscopy that, as an antibody against the hemagglutinin (HA) tag, marked PM ruffles and filopodia in HeLa cells with inducible expression of HA-DOCK10. |
| first_indexed | 2024-03-10T07:57:37Z |
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| id | doaj.art-e2fed27de01e42be90aa9d3c0076a919 |
| institution | Directory Open Access Journal |
| issn | 2073-4468 |
| language | English |
| last_indexed | 2024-03-10T07:57:37Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-e2fed27de01e42be90aa9d3c0076a9192023-11-22T11:47:01ZengMDPI AGAntibodies2073-44682021-08-011033310.3390/antib10030033Variable Distribution of DOCK-D Proteins between Cytosol and Nucleoplasm in Cell Lines, Effect of Interleukin-4 on DOCK10 in B-Cell Lymphoid Neoplasms, and Validation of a New DOCK10 Antiserum for Immunofluorescence StudiesNatalia Ruiz-Lafuente0Alfredo Minguela1Jose M. Moraleda2Manuel Muro3Antonio Parrado4Department of Immunology, University Clinical Hospital Virgen de la Arrixaca-Biomedical Research Institute of Murcia (IMIB-Arrixaca), 30120 Murcia, SpainDepartment of Immunology, University Clinical Hospital Virgen de la Arrixaca-Biomedical Research Institute of Murcia (IMIB-Arrixaca), 30120 Murcia, SpainDepartment of Hematology, University Clinical Hospital Virgen de la Arrixaca-Biomedical Research Institute of Murcia (IMIB-Arrixaca), 30120 Murcia, SpainDepartment of Immunology, University Clinical Hospital Virgen de la Arrixaca-Biomedical Research Institute of Murcia (IMIB-Arrixaca), 30120 Murcia, SpainDepartment of Immunology, University Clinical Hospital Virgen de la Arrixaca-Biomedical Research Institute of Murcia (IMIB-Arrixaca), 30120 Murcia, SpainDedicator-of-cytokinesis (DOCK), a family of guanine-nucleotide exchange factors (GEFs), comprises four subfamilies, named from A to D. DOCK-D comprises DOCK9, DOCK10, and DOCK11. The GEF activity involves translocation from the cytoplasm to the plasma membrane (PM), as assessed by the transfection of tagged proteins. However, the cellular localization of endogenous DOCK proteins is poorly understood. In this paper, to gain a better understanding of the role of the DOCK-D proteins, we studied their distribution between cytosol and nucleoplasm in 11 cell lines. DOCK-D proteins were distributed with variable cytosolic or nuclear predominance, although the latter was common for DOCK9 and DOCK11. These results suggest that the DOCK-D proteins may perform new nuclear functions, which remain to be discovered. Furthermore, we found that DOCK10 levels are increased by interleukin-4 (IL-4) in B-cell lymphoid neoplasms other than chronic lymphocytic leukemia (CLL) such as mantle cell lymphoma and diffuse large B-cell lymphoma. We also found evidence for an induction of the cytosolic levels of DOCK10 by IL-4 in CLL. Finally, we obtained a valid DOCK10 antiserum for immunofluorescence (IF) microscopy that, as an antibody against the hemagglutinin (HA) tag, marked PM ruffles and filopodia in HeLa cells with inducible expression of HA-DOCK10.https://www.mdpi.com/2073-4468/10/3/33DOCK10DOCK9DOCK11subcellular localizationB-cell lymphoid neoplasmsinterleukin-4 (IL-4) |
| spellingShingle | Natalia Ruiz-Lafuente Alfredo Minguela Jose M. Moraleda Manuel Muro Antonio Parrado Variable Distribution of DOCK-D Proteins between Cytosol and Nucleoplasm in Cell Lines, Effect of Interleukin-4 on DOCK10 in B-Cell Lymphoid Neoplasms, and Validation of a New DOCK10 Antiserum for Immunofluorescence Studies Antibodies DOCK10 DOCK9 DOCK11 subcellular localization B-cell lymphoid neoplasms interleukin-4 (IL-4) |
| title | Variable Distribution of DOCK-D Proteins between Cytosol and Nucleoplasm in Cell Lines, Effect of Interleukin-4 on DOCK10 in B-Cell Lymphoid Neoplasms, and Validation of a New DOCK10 Antiserum for Immunofluorescence Studies |
| title_full | Variable Distribution of DOCK-D Proteins between Cytosol and Nucleoplasm in Cell Lines, Effect of Interleukin-4 on DOCK10 in B-Cell Lymphoid Neoplasms, and Validation of a New DOCK10 Antiserum for Immunofluorescence Studies |
| title_fullStr | Variable Distribution of DOCK-D Proteins between Cytosol and Nucleoplasm in Cell Lines, Effect of Interleukin-4 on DOCK10 in B-Cell Lymphoid Neoplasms, and Validation of a New DOCK10 Antiserum for Immunofluorescence Studies |
| title_full_unstemmed | Variable Distribution of DOCK-D Proteins between Cytosol and Nucleoplasm in Cell Lines, Effect of Interleukin-4 on DOCK10 in B-Cell Lymphoid Neoplasms, and Validation of a New DOCK10 Antiserum for Immunofluorescence Studies |
| title_short | Variable Distribution of DOCK-D Proteins between Cytosol and Nucleoplasm in Cell Lines, Effect of Interleukin-4 on DOCK10 in B-Cell Lymphoid Neoplasms, and Validation of a New DOCK10 Antiserum for Immunofluorescence Studies |
| title_sort | variable distribution of dock d proteins between cytosol and nucleoplasm in cell lines effect of interleukin 4 on dock10 in b cell lymphoid neoplasms and validation of a new dock10 antiserum for immunofluorescence studies |
| topic | DOCK10 DOCK9 DOCK11 subcellular localization B-cell lymphoid neoplasms interleukin-4 (IL-4) |
| url | https://www.mdpi.com/2073-4468/10/3/33 |
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