Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatite
Abstract The present work studies the effect of Mn doping on the crystalline structure of the Hap synthesized by the hydrothermal method at 200 °C for 24 h, from Ca(OH)2 and (NH4)2HPO4, incorporating MnCl2 to 0.1, 0.5, 1.0, 1.5 and 2.0 %wt of Mn concentrations. Samples were characterized by the X-Ra...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2023-07-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-023-06744-0 |
| _version_ | 1797736441425428480 |
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| author | L. S. Villaseñor-Cerón D. Mendoza-Anaya S. López-Ortiz R. Rosales-Ibañez J. J. Rodríguez-Martínez M. I. Reyes-Valderrama V. Rodríguez-Lugo |
| author_facet | L. S. Villaseñor-Cerón D. Mendoza-Anaya S. López-Ortiz R. Rosales-Ibañez J. J. Rodríguez-Martínez M. I. Reyes-Valderrama V. Rodríguez-Lugo |
| author_sort | L. S. Villaseñor-Cerón |
| collection | DOAJ |
| description | Abstract The present work studies the effect of Mn doping on the crystalline structure of the Hap synthesized by the hydrothermal method at 200 °C for 24 h, from Ca(OH)2 and (NH4)2HPO4, incorporating MnCl2 to 0.1, 0.5, 1.0, 1.5 and 2.0 %wt of Mn concentrations. Samples were characterized by the X-Ray Diffraction technique, which revealed the diffraction peaks that corresponded to the hexagonal and monoclinic phase of the Hap; it was observed that the average size of crystallite decreased from 23.67 to 22.69 nm as the concentration of Mn increased. TEM shows that in all samples, there are two distributions of particle sizes; one corresponds to nanorods with several tens of nanometers in length, and the other in which the diameter and length are very close. FTIR analysis revealed absorption bands corresponding to the PO4 −3 and OH− groups characteristic of the Hap. It was possible to establish a substitution mechanism between the Mn and the ions of Ca+2 of the Hap. From the Alamar blue test, a cell viability of 86.88% ± 5 corresponding to the sample of Hap at 1.5 %wt Mn was obtained, considered non-cytotoxic according to ISO 10993-5. It also evaluated and demonstrated the good osteoinductive properties of the materials, which were verified by histology and immunofluorescence expression of osteogenic markers. Adhesion, viability, biocompatibility and osteoinductive properties, make these materials candidates for future applications in bone tissue engineering with likely uses in regenerative medicine. Graphical Abstract |
| first_indexed | 2024-03-12T13:13:58Z |
| format | Article |
| id | doaj.art-e304c5e68c974b3ba8c7ee69d5d6b69b |
| institution | Directory Open Access Journal |
| issn | 1573-4838 |
| language | English |
| last_indexed | 2024-03-12T13:13:58Z |
| publishDate | 2023-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj.art-e304c5e68c974b3ba8c7ee69d5d6b69b2023-08-27T11:07:59ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382023-07-0134811610.1007/s10856-023-06744-0Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatiteL. S. Villaseñor-Cerón0D. Mendoza-Anaya1S. López-Ortiz2R. Rosales-Ibañez3J. J. Rodríguez-Martínez4M. I. Reyes-Valderrama5V. Rodríguez-Lugo6Área Académica de Ciencias de la Tierra y Materiales, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5Instituto Nacional de Investigaciones Nucleares; Carr. México-Toluca s/n La MarquesaInstituto de Física, Universidad Autónoma de San Luis Potosí, Av. Parque Chapultepec1570, Privadas del PedregalLaboratorio de Ingeniería Tisular y Medicina Traslacional, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México. Avenida Tenayuca-Chalmita S/N, Cuautepec Barrio Bajo, Alcaldía Gustavo A. MaderoLaboratorio de Ingeniería Tisular y Medicina Traslacional, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México. Avenida Tenayuca-Chalmita S/N, Cuautepec Barrio Bajo, Alcaldía Gustavo A. MaderoÁrea Académica de Ciencias de la Tierra y Materiales, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5Área Académica de Ciencias de la Tierra y Materiales, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5Abstract The present work studies the effect of Mn doping on the crystalline structure of the Hap synthesized by the hydrothermal method at 200 °C for 24 h, from Ca(OH)2 and (NH4)2HPO4, incorporating MnCl2 to 0.1, 0.5, 1.0, 1.5 and 2.0 %wt of Mn concentrations. Samples were characterized by the X-Ray Diffraction technique, which revealed the diffraction peaks that corresponded to the hexagonal and monoclinic phase of the Hap; it was observed that the average size of crystallite decreased from 23.67 to 22.69 nm as the concentration of Mn increased. TEM shows that in all samples, there are two distributions of particle sizes; one corresponds to nanorods with several tens of nanometers in length, and the other in which the diameter and length are very close. FTIR analysis revealed absorption bands corresponding to the PO4 −3 and OH− groups characteristic of the Hap. It was possible to establish a substitution mechanism between the Mn and the ions of Ca+2 of the Hap. From the Alamar blue test, a cell viability of 86.88% ± 5 corresponding to the sample of Hap at 1.5 %wt Mn was obtained, considered non-cytotoxic according to ISO 10993-5. It also evaluated and demonstrated the good osteoinductive properties of the materials, which were verified by histology and immunofluorescence expression of osteogenic markers. Adhesion, viability, biocompatibility and osteoinductive properties, make these materials candidates for future applications in bone tissue engineering with likely uses in regenerative medicine. Graphical Abstracthttps://doi.org/10.1007/s10856-023-06744-0 |
| spellingShingle | L. S. Villaseñor-Cerón D. Mendoza-Anaya S. López-Ortiz R. Rosales-Ibañez J. J. Rodríguez-Martínez M. I. Reyes-Valderrama V. Rodríguez-Lugo Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatite Journal of Materials Science: Materials in Medicine |
| title | Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatite |
| title_full | Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatite |
| title_fullStr | Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatite |
| title_full_unstemmed | Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatite |
| title_short | Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatite |
| title_sort | biocompatibility analysis and chemical characterization of mn doped hydroxyapatite |
| url | https://doi.org/10.1007/s10856-023-06744-0 |
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