Bioassay for investigation of auxin transport in single cell layers
Auxin was collected from the cambial region of Pinus sylvestris by applying agar strips to the cut surfaces of stem sections which comprised a single layer of 2 to 4-mm long, mainly intact fusiform cells. Sections of the agar strips were either bioassayed immediately to determine their auxin content...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2014-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/896 |
| _version_ | 1818239108460314624 |
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| author | Alina B. Wodzicki Sylwia Gessel Tomasz J. Wodzicki |
| author_facet | Alina B. Wodzicki Sylwia Gessel Tomasz J. Wodzicki |
| author_sort | Alina B. Wodzicki |
| collection | DOAJ |
| description | Auxin was collected from the cambial region of Pinus sylvestris by applying agar strips to the cut surfaces of stem sections which comprised a single layer of 2 to 4-mm long, mainly intact fusiform cells. Sections of the agar strips were either bioassayed immediately to determine their auxin content or stored for several months at -80oC, extracted with 80% MeOH and redissolved in hot agar prior to bioassay. Auxin concentrations were determined by Went's oat coleoptile test, as described by Funke, which was modified considerably to give highly reproducible results. The modifications proved essential for good replication of results and are described in detail together with the use of the bioassay to determine changes in cambial cell polarity during ageing and senescence in P. sylvestris. |
| first_indexed | 2024-12-12T12:52:18Z |
| format | Article |
| id | doaj.art-e30681b69631438387863dc05e5d8b81 |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-12T12:52:18Z |
| publishDate | 2014-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-e30681b69631438387863dc05e5d8b812022-12-22T00:23:58ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-01663-435536410.5586/asbp.1997.044669Bioassay for investigation of auxin transport in single cell layersAlina B. Wodzicki0Sylwia Gessel1Tomasz J. Wodzicki2Agricultural University of WarsawAgricultural University of WarsawAgricultural University of WarsawAuxin was collected from the cambial region of Pinus sylvestris by applying agar strips to the cut surfaces of stem sections which comprised a single layer of 2 to 4-mm long, mainly intact fusiform cells. Sections of the agar strips were either bioassayed immediately to determine their auxin content or stored for several months at -80oC, extracted with 80% MeOH and redissolved in hot agar prior to bioassay. Auxin concentrations were determined by Went's oat coleoptile test, as described by Funke, which was modified considerably to give highly reproducible results. The modifications proved essential for good replication of results and are described in detail together with the use of the bioassay to determine changes in cambial cell polarity during ageing and senescence in P. sylvestris.https://pbsociety.org.pl/journals/index.php/asbp/article/view/896bioassayauxintransport polaritycambiumPinus sylvestris |
| spellingShingle | Alina B. Wodzicki Sylwia Gessel Tomasz J. Wodzicki Bioassay for investigation of auxin transport in single cell layers Acta Societatis Botanicorum Poloniae bioassay auxin transport polarity cambium Pinus sylvestris |
| title | Bioassay for investigation of auxin transport in single cell layers |
| title_full | Bioassay for investigation of auxin transport in single cell layers |
| title_fullStr | Bioassay for investigation of auxin transport in single cell layers |
| title_full_unstemmed | Bioassay for investigation of auxin transport in single cell layers |
| title_short | Bioassay for investigation of auxin transport in single cell layers |
| title_sort | bioassay for investigation of auxin transport in single cell layers |
| topic | bioassay auxin transport polarity cambium Pinus sylvestris |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/896 |
| work_keys_str_mv | AT alinabwodzicki bioassayforinvestigationofauxintransportinsinglecelllayers AT sylwiagessel bioassayforinvestigationofauxintransportinsinglecelllayers AT tomaszjwodzicki bioassayforinvestigationofauxintransportinsinglecelllayers |