Poxvirus Infections in Dairy Farms and Transhumance Cattle Herds in Nigeria
Lumpy Skin disease (LSD) is an economically important disease in cattle caused by the LSD virus (LSDV) of the genus <i>Capripoxvirus</i>, while pseudocowpox (PCP) is a widely distributed zoonotic cattle disease caused by the PCP virus (PCPV) of the genus <i>Parapoxvirus</i>....
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MDPI AG
2023-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/5/1051 |
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| author | David Oludare Omoniwa Irene Kasindi Meki Caleb Ayuba Kudi Anthony Kojo Sackey Maryam Aminu Adeyinka Jeremy Adedeji Clement Adebajo Meseko Pam Dachung Luka Olayinka Oluwafemi Asala Jolly Amoche Adole Rebecca Bitiyong Atai Yakubu Joel Atuman Tirumala Bharani Kumar Settypalli Giovanni Cattoli Charles Euloge Lamien |
| author_facet | David Oludare Omoniwa Irene Kasindi Meki Caleb Ayuba Kudi Anthony Kojo Sackey Maryam Aminu Adeyinka Jeremy Adedeji Clement Adebajo Meseko Pam Dachung Luka Olayinka Oluwafemi Asala Jolly Amoche Adole Rebecca Bitiyong Atai Yakubu Joel Atuman Tirumala Bharani Kumar Settypalli Giovanni Cattoli Charles Euloge Lamien |
| author_sort | David Oludare Omoniwa |
| collection | DOAJ |
| description | Lumpy Skin disease (LSD) is an economically important disease in cattle caused by the LSD virus (LSDV) of the genus <i>Capripoxvirus</i>, while pseudocowpox (PCP) is a widely distributed zoonotic cattle disease caused by the PCP virus (PCPV) of the genus <i>Parapoxvirus</i>. Though both viral pox infections are reportedly present in Nigeria, similarities in their clinical presentation and limited access to laboratories often lead to misdiagnosis in the field. This study investigated suspected LSD outbreaks in organized and transhumance cattle herds in Nigeria in 2020. A total of 42 scab/skin biopsy samples were collected from 16 outbreaks of suspected LSD in five northern States of Nigeria. The samples were analyzed using a high-resolution multiplex melting (HRM) assay to differentiate poxviruses belonging to <i>Orthopoxvirus</i>, <i>Capripoxvirus</i>, and <i>Parapoxvirus</i> genera. LSDV was characterized using four gene segments, namely the RNA polymerase 30 kDa subunit (RPO30), G-protein-coupled receptor (GPCR), the extracellular enveloped virus (EEV) glycoprotein and CaPV homolog of the variola virus B22R. Likewise, the partial B2L gene of PCPV was also analyzed. Nineteen samples (45.2%) were positive according to the HRM assay for LSDV, and five (11.9%) were co-infected with LSDV and PCPV. The multiple sequence alignments of the GPCR, EEV, and B22R showed 100% similarity among the Nigerian LSDV samples, unlike the RPO30 phylogeny, which showed two clusters. Some of the Nigerian LSDVs clustered within LSDV SG II were with commonly circulating LSDV field isolates in Africa, the Middle East, and Europe, while the remaining Nigerian LSDVs produced a unique sub-group. The B2L sequences of Nigerian PCPVs were 100% identical and clustered within the PCPV group containing cattle/Reindeer isolates, close to PCPVs from Zambia and Botswana. The results show the diversity of Nigerian LSDV strains. This paper also reports the first documented co-infection of LSDV and PCPV in Nigeria. |
| first_indexed | 2024-03-11T03:14:53Z |
| format | Article |
| id | doaj.art-e3075252c09b4c0583cf1ab60042a806 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T03:14:53Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e3075252c09b4c0583cf1ab60042a8062023-11-18T03:38:13ZengMDPI AGViruses1999-49152023-04-01155105110.3390/v15051051Poxvirus Infections in Dairy Farms and Transhumance Cattle Herds in NigeriaDavid Oludare Omoniwa0Irene Kasindi Meki1Caleb Ayuba Kudi2Anthony Kojo Sackey3Maryam Aminu4Adeyinka Jeremy Adedeji5Clement Adebajo Meseko6Pam Dachung Luka7Olayinka Oluwafemi Asala8Jolly Amoche Adole9Rebecca Bitiyong Atai10Yakubu Joel Atuman11Tirumala Bharani Kumar Settypalli12Giovanni Cattoli13Charles Euloge Lamien14Department of Veterinary Medicine, Surgery and Radiology, University of Jos, Jos 930001, Plateau State, NigeriaAnimal Production and Health Laboratory, Animal Production and Health Section, Joint FAO/IAEA Division, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, AustriaDepartment of Veterinary Medicine, Ahmadu Bello University, Zaria 810211, Kaduna State, NigeriaDepartment of Veterinary Medicine, Ahmadu Bello University, Zaria 810211, Kaduna State, NigeriaDepartment of Microbiology, Ahmadu Bello University, Zaria 810211, Kaduna State, NigeriaNational Veterinary Research Institute, Vom 930103, Plateau State, NigeriaNational Veterinary Research Institute, Vom 930103, Plateau State, NigeriaNational Veterinary Research Institute, Vom 930103, Plateau State, NigeriaNational Veterinary Research Institute, Vom 930103, Plateau State, NigeriaNational Veterinary Research Institute, Vom 930103, Plateau State, NigeriaNational Veterinary Research Institute, Vom 930103, Plateau State, NigeriaNational Veterinary Research Institute, Vom 930103, Plateau State, NigeriaAnimal Production and Health Laboratory, Animal Production and Health Section, Joint FAO/IAEA Division, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, AustriaAnimal Production and Health Laboratory, Animal Production and Health Section, Joint FAO/IAEA Division, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, AustriaAnimal Production and Health Laboratory, Animal Production and Health Section, Joint FAO/IAEA Division, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, P.O. Box 100, 1400 Vienna, AustriaLumpy Skin disease (LSD) is an economically important disease in cattle caused by the LSD virus (LSDV) of the genus <i>Capripoxvirus</i>, while pseudocowpox (PCP) is a widely distributed zoonotic cattle disease caused by the PCP virus (PCPV) of the genus <i>Parapoxvirus</i>. Though both viral pox infections are reportedly present in Nigeria, similarities in their clinical presentation and limited access to laboratories often lead to misdiagnosis in the field. This study investigated suspected LSD outbreaks in organized and transhumance cattle herds in Nigeria in 2020. A total of 42 scab/skin biopsy samples were collected from 16 outbreaks of suspected LSD in five northern States of Nigeria. The samples were analyzed using a high-resolution multiplex melting (HRM) assay to differentiate poxviruses belonging to <i>Orthopoxvirus</i>, <i>Capripoxvirus</i>, and <i>Parapoxvirus</i> genera. LSDV was characterized using four gene segments, namely the RNA polymerase 30 kDa subunit (RPO30), G-protein-coupled receptor (GPCR), the extracellular enveloped virus (EEV) glycoprotein and CaPV homolog of the variola virus B22R. Likewise, the partial B2L gene of PCPV was also analyzed. Nineteen samples (45.2%) were positive according to the HRM assay for LSDV, and five (11.9%) were co-infected with LSDV and PCPV. The multiple sequence alignments of the GPCR, EEV, and B22R showed 100% similarity among the Nigerian LSDV samples, unlike the RPO30 phylogeny, which showed two clusters. Some of the Nigerian LSDVs clustered within LSDV SG II were with commonly circulating LSDV field isolates in Africa, the Middle East, and Europe, while the remaining Nigerian LSDVs produced a unique sub-group. The B2L sequences of Nigerian PCPVs were 100% identical and clustered within the PCPV group containing cattle/Reindeer isolates, close to PCPVs from Zambia and Botswana. The results show the diversity of Nigerian LSDV strains. This paper also reports the first documented co-infection of LSDV and PCPV in Nigeria.https://www.mdpi.com/1999-4915/15/5/1051poxvirusdairy farmstranshumancescattleco-infectionpseudocowpox |
| spellingShingle | David Oludare Omoniwa Irene Kasindi Meki Caleb Ayuba Kudi Anthony Kojo Sackey Maryam Aminu Adeyinka Jeremy Adedeji Clement Adebajo Meseko Pam Dachung Luka Olayinka Oluwafemi Asala Jolly Amoche Adole Rebecca Bitiyong Atai Yakubu Joel Atuman Tirumala Bharani Kumar Settypalli Giovanni Cattoli Charles Euloge Lamien Poxvirus Infections in Dairy Farms and Transhumance Cattle Herds in Nigeria Viruses poxvirus dairy farms transhumances cattle co-infection pseudocowpox |
| title | Poxvirus Infections in Dairy Farms and Transhumance Cattle Herds in Nigeria |
| title_full | Poxvirus Infections in Dairy Farms and Transhumance Cattle Herds in Nigeria |
| title_fullStr | Poxvirus Infections in Dairy Farms and Transhumance Cattle Herds in Nigeria |
| title_full_unstemmed | Poxvirus Infections in Dairy Farms and Transhumance Cattle Herds in Nigeria |
| title_short | Poxvirus Infections in Dairy Farms and Transhumance Cattle Herds in Nigeria |
| title_sort | poxvirus infections in dairy farms and transhumance cattle herds in nigeria |
| topic | poxvirus dairy farms transhumances cattle co-infection pseudocowpox |
| url | https://www.mdpi.com/1999-4915/15/5/1051 |
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