β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds
Alperujo—a two-phase olive mill waste that is composed of olive vegetation water and solid skin, pulp, and seed fragments - is a highly valuable olive by-product due to its high content in phenolic compounds. In this study, we assessed whether β-cyclodextrin (β-CD), which...
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| Format: | Article |
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MDPI AG
2018-11-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/10/11/1653 |
| _version_ | 1811291046652411904 |
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| author | Aurélia Malapert Valérie Tomao Marielle Margier Marion Nowicki Béatrice Gleize Olivier Dangles Emmanuelle Reboul |
| author_facet | Aurélia Malapert Valérie Tomao Marielle Margier Marion Nowicki Béatrice Gleize Olivier Dangles Emmanuelle Reboul |
| author_sort | Aurélia Malapert |
| collection | DOAJ |
| description | Alperujo—a two-phase olive mill waste that is composed of olive vegetation water and solid skin, pulp, and seed fragments - is a highly valuable olive by-product due to its high content in phenolic compounds. In this study, we assessed whether β-cyclodextrin (β-CD), which is used to extract and protect alpejuro phenolic compounds (hydroxytyrosol-<i>O</i>-glucoside, tyrosol, caffeic, and <i>p</i>-coumaric acids) could impact on their bioaccessibility (i.e., the percentage of molecule found in the aqueous phase of the digesta) and uptake by intestinal cells, by using an in vitro digestion model and Caco-2 TC7 cells in culture, respectively. Our results showed that β-CD did not change the bioaccessibility of the selected phenols. Hydroxytyrosol-<i>O</i>-glucoside and caffeic did not cross Caco-2 cell monolayers. Conversely ferulic acid, identified as the main caffeic acid intestinal metabolite, was absorbed through intestinal cell monolayers (~20%). Interestingly, β-CD moderately but significantly improved the local absorption of tyrosol and <i>p</i>-coumaric acid (2.3 + 1.4% and 8.5 ± 4.2%, respectively, <i>p</i> < 0.05), even if their final bioavailability (expressed as bioaccessibility × absorption by Caco-2 cells) was not modified (16.2 ± 0.6% vs. 16.8 ± 0.5% for tyrosol and 32.0 ± 3.2% vs. 37.2 ± 3.2% for <i>p</i>-coumaric acid, from pure alperujo and alperujo complexed with β-CD, respectively). Overall, our results show that β-CD is an interesting extraction and storage agent for phenolic compounds that does not alter their in vitro bioavailability. |
| first_indexed | 2024-04-13T04:22:36Z |
| format | Article |
| id | doaj.art-e30990d367784e19b7dd81a6cc562127 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-04-13T04:22:36Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-e30990d367784e19b7dd81a6cc5621272022-12-22T03:02:40ZengMDPI AGNutrients2072-66432018-11-011011165310.3390/nu10111653nu10111653β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic CompoundsAurélia Malapert0Valérie Tomao1Marielle Margier2Marion Nowicki3Béatrice Gleize4Olivier Dangles5Emmanuelle Reboul6Avignon University, INRA, UMR408 SQPOV, F-84000 Avignon, FranceAvignon University, INRA, UMR408 SQPOV, F-84000 Avignon, FranceAix-Marseille University, INRA, INSERM, C2VN, 13385 Marseille, FranceAix-Marseille University, INRA, INSERM, C2VN, 13385 Marseille, FranceAvignon University, INRA, UMR408 SQPOV, F-84000 Avignon, FranceAvignon University, INRA, UMR408 SQPOV, F-84000 Avignon, FranceAix-Marseille University, INRA, INSERM, C2VN, 13385 Marseille, FranceAlperujo—a two-phase olive mill waste that is composed of olive vegetation water and solid skin, pulp, and seed fragments - is a highly valuable olive by-product due to its high content in phenolic compounds. In this study, we assessed whether β-cyclodextrin (β-CD), which is used to extract and protect alpejuro phenolic compounds (hydroxytyrosol-<i>O</i>-glucoside, tyrosol, caffeic, and <i>p</i>-coumaric acids) could impact on their bioaccessibility (i.e., the percentage of molecule found in the aqueous phase of the digesta) and uptake by intestinal cells, by using an in vitro digestion model and Caco-2 TC7 cells in culture, respectively. Our results showed that β-CD did not change the bioaccessibility of the selected phenols. Hydroxytyrosol-<i>O</i>-glucoside and caffeic did not cross Caco-2 cell monolayers. Conversely ferulic acid, identified as the main caffeic acid intestinal metabolite, was absorbed through intestinal cell monolayers (~20%). Interestingly, β-CD moderately but significantly improved the local absorption of tyrosol and <i>p</i>-coumaric acid (2.3 + 1.4% and 8.5 ± 4.2%, respectively, <i>p</i> < 0.05), even if their final bioavailability (expressed as bioaccessibility × absorption by Caco-2 cells) was not modified (16.2 ± 0.6% vs. 16.8 ± 0.5% for tyrosol and 32.0 ± 3.2% vs. 37.2 ± 3.2% for <i>p</i>-coumaric acid, from pure alperujo and alperujo complexed with β-CD, respectively). Overall, our results show that β-CD is an interesting extraction and storage agent for phenolic compounds that does not alter their in vitro bioavailability.https://www.mdpi.com/2072-6643/10/11/1653hydroxytyrosol-<i>O</i>-glucosidetyrosolcaffeic acid<i>p</i>-coumaric acidalperujoolive pomacepolyphenolsbioavailabilityin vitro digestionenterocytes |
| spellingShingle | Aurélia Malapert Valérie Tomao Marielle Margier Marion Nowicki Béatrice Gleize Olivier Dangles Emmanuelle Reboul β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds Nutrients hydroxytyrosol-<i>O</i>-glucoside tyrosol caffeic acid <i>p</i>-coumaric acid alperujo olive pomace polyphenols bioavailability in vitro digestion enterocytes |
| title | β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds |
| title_full | β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds |
| title_fullStr | β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds |
| title_full_unstemmed | β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds |
| title_short | β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds |
| title_sort | β cyclodextrin does not alter the bioaccessibility and the uptake by caco 2 cells of olive by product phenolic compounds |
| topic | hydroxytyrosol-<i>O</i>-glucoside tyrosol caffeic acid <i>p</i>-coumaric acid alperujo olive pomace polyphenols bioavailability in vitro digestion enterocytes |
| url | https://www.mdpi.com/2072-6643/10/11/1653 |
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