Effect of Electrolyte Concentration on Cell Sensing by Measuring Ionic Current Waveform through Micropores
Immunostaining has been widely used in cancer prognosis for the quantitative detection of cancer cells present in the bloodstream. However, conventional detection methods based on the target membrane protein expression exhibit the risk of missing cancer cells owing to variable protein expressions. I...
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MDPI AG
2021-03-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/3/78 |
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| author | Kazumichi Yokota Muneaki Hashimoto Kazuaki Kajimoto Masato Tanaka Sanae Murayama Makusu Tsutsui Yoshihiro Nakajima Masateru Taniguchi Masatoshi Kataoka |
| author_facet | Kazumichi Yokota Muneaki Hashimoto Kazuaki Kajimoto Masato Tanaka Sanae Murayama Makusu Tsutsui Yoshihiro Nakajima Masateru Taniguchi Masatoshi Kataoka |
| author_sort | Kazumichi Yokota |
| collection | DOAJ |
| description | Immunostaining has been widely used in cancer prognosis for the quantitative detection of cancer cells present in the bloodstream. However, conventional detection methods based on the target membrane protein expression exhibit the risk of missing cancer cells owing to variable protein expressions. In this study, the resistive pulse method (RPM) was employed to discriminate between cultured cancer cells (NCI-H1650) and T lymphoblastoid leukemia cells (CCRF-CEM) by measuring the ionic current response of cells flowing through a micro-space. The height and shape of a pulse signal were used for the simultaneous measurement of size, deformability, and surface charge of individual cells. An accurate discrimination of cancer cells could not be obtained using 1.0 × phosphate-buffered saline (PBS) as an electrolyte solution to compare the size measurements by a microscopic observation. However, an accurate discrimination of cancer cells with a discrimination error rate of 4.5 ± 0.5% was achieved using 0.5 × PBS containing 2.77% glucose as the electrolyte solution. The potential application of RPM for the accurate discrimination of cancer cells from leukocytes was demonstrated through the measurement of the individual cell size, deformability, and surface charge in a solution with a low electrolyte concentration. |
| first_indexed | 2024-03-10T13:16:53Z |
| format | Article |
| id | doaj.art-e309ccc51b3a4c2cbb4ab1e581c7ef2e |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T13:16:53Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-e309ccc51b3a4c2cbb4ab1e581c7ef2e2023-11-21T10:19:45ZengMDPI AGBiosensors2079-63742021-03-011137810.3390/bios11030078Effect of Electrolyte Concentration on Cell Sensing by Measuring Ionic Current Waveform through MicroporesKazumichi Yokota0Muneaki Hashimoto1Kazuaki Kajimoto2Masato Tanaka3Sanae Murayama4Makusu Tsutsui5Yoshihiro Nakajima6Masateru Taniguchi7Masatoshi Kataoka8National Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa 761-0395, JapanNational Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa 761-0395, JapanNational Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa 761-0395, JapanNational Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa 761-0395, JapanThe Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, JapanThe Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, JapanNational Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa 761-0395, JapanThe Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, JapanNational Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa 761-0395, JapanImmunostaining has been widely used in cancer prognosis for the quantitative detection of cancer cells present in the bloodstream. However, conventional detection methods based on the target membrane protein expression exhibit the risk of missing cancer cells owing to variable protein expressions. In this study, the resistive pulse method (RPM) was employed to discriminate between cultured cancer cells (NCI-H1650) and T lymphoblastoid leukemia cells (CCRF-CEM) by measuring the ionic current response of cells flowing through a micro-space. The height and shape of a pulse signal were used for the simultaneous measurement of size, deformability, and surface charge of individual cells. An accurate discrimination of cancer cells could not be obtained using 1.0 × phosphate-buffered saline (PBS) as an electrolyte solution to compare the size measurements by a microscopic observation. However, an accurate discrimination of cancer cells with a discrimination error rate of 4.5 ± 0.5% was achieved using 0.5 × PBS containing 2.77% glucose as the electrolyte solution. The potential application of RPM for the accurate discrimination of cancer cells from leukocytes was demonstrated through the measurement of the individual cell size, deformability, and surface charge in a solution with a low electrolyte concentration.https://www.mdpi.com/2079-6374/11/3/78resistive pulse methodcell discriminationcancer cellleukocytemicropore |
| spellingShingle | Kazumichi Yokota Muneaki Hashimoto Kazuaki Kajimoto Masato Tanaka Sanae Murayama Makusu Tsutsui Yoshihiro Nakajima Masateru Taniguchi Masatoshi Kataoka Effect of Electrolyte Concentration on Cell Sensing by Measuring Ionic Current Waveform through Micropores Biosensors resistive pulse method cell discrimination cancer cell leukocyte micropore |
| title | Effect of Electrolyte Concentration on Cell Sensing by Measuring Ionic Current Waveform through Micropores |
| title_full | Effect of Electrolyte Concentration on Cell Sensing by Measuring Ionic Current Waveform through Micropores |
| title_fullStr | Effect of Electrolyte Concentration on Cell Sensing by Measuring Ionic Current Waveform through Micropores |
| title_full_unstemmed | Effect of Electrolyte Concentration on Cell Sensing by Measuring Ionic Current Waveform through Micropores |
| title_short | Effect of Electrolyte Concentration on Cell Sensing by Measuring Ionic Current Waveform through Micropores |
| title_sort | effect of electrolyte concentration on cell sensing by measuring ionic current waveform through micropores |
| topic | resistive pulse method cell discrimination cancer cell leukocyte micropore |
| url | https://www.mdpi.com/2079-6374/11/3/78 |
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