Painful Terminal Neuroma Prevention by Capping PRGD/PDLLA Conduit in Rat Sciatic Nerves
Abstract Neuroma formation after amputation as a long‐term deficiency leads to spontaneous neuropathic pain that reduces quality of life of patients. To prevent neuroma formation, capping techniques are implemented as effective treatments. However, an ideal, biocompatible material covering the nerve...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-06-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.201700876 |
| _version_ | 1797754270904221696 |
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| author | Jiling Yi Nan Jiang Binbin Li Qiongjiao Yan Tong Qiu Killugudi Swaminatha Iyer Yixia Yin Honglian Dai Ali K. Yetisen Shipu Li |
| author_facet | Jiling Yi Nan Jiang Binbin Li Qiongjiao Yan Tong Qiu Killugudi Swaminatha Iyer Yixia Yin Honglian Dai Ali K. Yetisen Shipu Li |
| author_sort | Jiling Yi |
| collection | DOAJ |
| description | Abstract Neuroma formation after amputation as a long‐term deficiency leads to spontaneous neuropathic pain that reduces quality of life of patients. To prevent neuroma formation, capping techniques are implemented as effective treatments. However, an ideal, biocompatible material covering the nerves is an unmet clinical need. In this study, biocompatible characteristics presented by the poly(D,L‐lactic acid)/arginylglycylaspartic acid (RGD peptide) modification of poly{(lactic acid)‐co‐ [(glycolic acid)‐alt‐(L‐lysine)]} (PRGD/PDLLA) are evaluated as a nerve conduit. After being capped on the rat sciatic nerve stump in vivo, rodent behaviors and tissue structures are compared via autotomy scoring and histological analyses. The PRGD/PDLLA capped group gains lower autotomy score and improves the recovery, where inflammatory infiltrations and excessive collagen deposition are defeated. Transmission electron microscopy images of the regeneration of myelin sheath in both groups show that abnormal myelination is only present in the uncapped rats. Changes in related genes (MPZ, MBP, MAG, and Krox20) are monitored quantitative real‐time polymerase chain reaction (qRT‐PCR) for mechanism investigation. The PRGD/PDLLA capping conduits not only act as physical barriers to inhibit the invasion of inflammatory infiltration in the scar tissue but also provide a suitable microenvironment for promoting nerve repairing and avoiding neuroma formation during nerve recovery. |
| first_indexed | 2024-03-12T17:30:06Z |
| format | Article |
| id | doaj.art-e30cef5c2d2b4c8bbadc58bf2dc8d60f |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-12T17:30:06Z |
| publishDate | 2018-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-e30cef5c2d2b4c8bbadc58bf2dc8d60f2023-08-05T03:41:20ZengWileyAdvanced Science2198-38442018-06-0156n/an/a10.1002/advs.201700876Painful Terminal Neuroma Prevention by Capping PRGD/PDLLA Conduit in Rat Sciatic NervesJiling Yi0Nan Jiang1Binbin Li2Qiongjiao Yan3Tong Qiu4Killugudi Swaminatha Iyer5Yixia Yin6Honglian Dai7Ali K. Yetisen8Shipu Li9State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 ChinaState Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 ChinaState Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 ChinaState Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 ChinaState Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 ChinaSchool of Molecular Sciences University of Western Australia 35 Stirling Hwy Crawley WA 6009 AustraliaState Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 ChinaState Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 ChinaSchool of Chemical Engineering University of Birmingham Edgbaston Birmingham B15 2TT UKState Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 ChinaAbstract Neuroma formation after amputation as a long‐term deficiency leads to spontaneous neuropathic pain that reduces quality of life of patients. To prevent neuroma formation, capping techniques are implemented as effective treatments. However, an ideal, biocompatible material covering the nerves is an unmet clinical need. In this study, biocompatible characteristics presented by the poly(D,L‐lactic acid)/arginylglycylaspartic acid (RGD peptide) modification of poly{(lactic acid)‐co‐ [(glycolic acid)‐alt‐(L‐lysine)]} (PRGD/PDLLA) are evaluated as a nerve conduit. After being capped on the rat sciatic nerve stump in vivo, rodent behaviors and tissue structures are compared via autotomy scoring and histological analyses. The PRGD/PDLLA capped group gains lower autotomy score and improves the recovery, where inflammatory infiltrations and excessive collagen deposition are defeated. Transmission electron microscopy images of the regeneration of myelin sheath in both groups show that abnormal myelination is only present in the uncapped rats. Changes in related genes (MPZ, MBP, MAG, and Krox20) are monitored quantitative real‐time polymerase chain reaction (qRT‐PCR) for mechanism investigation. The PRGD/PDLLA capping conduits not only act as physical barriers to inhibit the invasion of inflammatory infiltration in the scar tissue but also provide a suitable microenvironment for promoting nerve repairing and avoiding neuroma formation during nerve recovery.https://doi.org/10.1002/advs.201700876inflammationnerve conduitsneuroma preventionpainful scar neuropathyscar deposition |
| spellingShingle | Jiling Yi Nan Jiang Binbin Li Qiongjiao Yan Tong Qiu Killugudi Swaminatha Iyer Yixia Yin Honglian Dai Ali K. Yetisen Shipu Li Painful Terminal Neuroma Prevention by Capping PRGD/PDLLA Conduit in Rat Sciatic Nerves Advanced Science inflammation nerve conduits neuroma prevention painful scar neuropathy scar deposition |
| title | Painful Terminal Neuroma Prevention by Capping PRGD/PDLLA Conduit in Rat Sciatic Nerves |
| title_full | Painful Terminal Neuroma Prevention by Capping PRGD/PDLLA Conduit in Rat Sciatic Nerves |
| title_fullStr | Painful Terminal Neuroma Prevention by Capping PRGD/PDLLA Conduit in Rat Sciatic Nerves |
| title_full_unstemmed | Painful Terminal Neuroma Prevention by Capping PRGD/PDLLA Conduit in Rat Sciatic Nerves |
| title_short | Painful Terminal Neuroma Prevention by Capping PRGD/PDLLA Conduit in Rat Sciatic Nerves |
| title_sort | painful terminal neuroma prevention by capping prgd pdlla conduit in rat sciatic nerves |
| topic | inflammation nerve conduits neuroma prevention painful scar neuropathy scar deposition |
| url | https://doi.org/10.1002/advs.201700876 |
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