IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract
Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. How...
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Associação Brasileira de Divulgação Científica
2004-06-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600006 |
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| author | M. Maciel A.E. Fusaro C.R. Oliveira E.A. Futata A.J.S. Duarte M.N. Sato |
| author_facet | M. Maciel A.E. Fusaro C.R. Oliveira E.A. Futata A.J.S. Duarte M.N. Sato |
| author_sort | M. Maciel |
| collection | DOAJ |
| description | Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp) to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-ß secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old) fed by gavage a total weight of 1.0-mg Dp extract on the 6th, 7th and 8th days post-immunization presented normal secretion of IL-4 and IL-10 in gut-associated lymphoid tissue and a decreased production of interferon gamma induced by Dp in the draining lymph nodes (13,340 ± 3,519 vs 29,280 ± 2,971 pg/ml). Mice fed the Dp extract also showed higher levels of serum anti-Dp IgA antibodies and an increase of IgA-secreting cells in mesenteric lymph nodes (N = 10), reflecting an increase in total fecal IgA antibodies (N = 10). The levels of secretory anti-Dp IgA antibodies increased after re-immunization regardless of Dp extract feeding. Oral tolerance did not interfere with serum or secretory IgA antibody reactivity related to self and non-self antigens. These results suggest that induction of oral tolerance to a Dp extract in sensitized mice triggered different regulatory mechanisms which inhibited the IgE response and stimulated systemic and secretory IgA responses, preserving the natural polyreactive IgA antibody production. |
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| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-19T08:02:03Z |
| publishDate | 2004-06-01 |
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| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-e30fba2a12dc4ce983aa8ffed8529cd22022-12-21T20:29:50ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2004-06-0137681782610.1590/S0100-879X2004000600006IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extractM. MacielA.E. FusaroC.R. OliveiraE.A. FutataA.J.S. DuarteM.N. SatoInduced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp) to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-ß secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old) fed by gavage a total weight of 1.0-mg Dp extract on the 6th, 7th and 8th days post-immunization presented normal secretion of IL-4 and IL-10 in gut-associated lymphoid tissue and a decreased production of interferon gamma induced by Dp in the draining lymph nodes (13,340 ± 3,519 vs 29,280 ± 2,971 pg/ml). Mice fed the Dp extract also showed higher levels of serum anti-Dp IgA antibodies and an increase of IgA-secreting cells in mesenteric lymph nodes (N = 10), reflecting an increase in total fecal IgA antibodies (N = 10). The levels of secretory anti-Dp IgA antibodies increased after re-immunization regardless of Dp extract feeding. Oral tolerance did not interfere with serum or secretory IgA antibody reactivity related to self and non-self antigens. These results suggest that induction of oral tolerance to a Dp extract in sensitized mice triggered different regulatory mechanisms which inhibited the IgE response and stimulated systemic and secretory IgA responses, preserving the natural polyreactive IgA antibody production.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600006Mucosaatural IgA antibodiesOral toleranceCytokinesAllergyDermatophagoides pteronyssinus |
| spellingShingle | M. Maciel A.E. Fusaro C.R. Oliveira E.A. Futata A.J.S. Duarte M.N. Sato IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract Brazilian Journal of Medical and Biological Research Mucosa atural IgA antibodies Oral tolerance Cytokines Allergy Dermatophagoides pteronyssinus |
| title | IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract |
| title_full | IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract |
| title_fullStr | IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract |
| title_full_unstemmed | IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract |
| title_short | IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract |
| title_sort | iga response in serum and gut secretion in sensitized mice fed with the dust mite dermatophagoides pteronyssinus extract |
| topic | Mucosa atural IgA antibodies Oral tolerance Cytokines Allergy Dermatophagoides pteronyssinus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600006 |
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