An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking
Most of the probiotics Bacterial cells, express native antibacterial genes, resulting in the production of, antimicrobial peptides, which have various applications in biotechnology and drug development. But the identification of antibacterial peptide, structural characterization of antimicrobial pep...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340920306399 |
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| author | Ramachandran Chelliah Kandasamy Saravanakumar Eric Banan-Mwine Daliri Joong-Hark Kim Jung-Kun Lee Hyeon-yeong Jo Inamul Hasan Madar Se-Hun Kim Sudha Rani Ramakrishnan Momna Rubab Kaliyan Barathikannan Fred Kwame Ofosu Hwang Subin Park Eun-ji Fazle Elahi Myeong-Hyeon Wang Deog-Hwan Oh |
| author_facet | Ramachandran Chelliah Kandasamy Saravanakumar Eric Banan-Mwine Daliri Joong-Hark Kim Jung-Kun Lee Hyeon-yeong Jo Inamul Hasan Madar Se-Hun Kim Sudha Rani Ramakrishnan Momna Rubab Kaliyan Barathikannan Fred Kwame Ofosu Hwang Subin Park Eun-ji Fazle Elahi Myeong-Hyeon Wang Deog-Hwan Oh |
| author_sort | Ramachandran Chelliah |
| collection | DOAJ |
| description | Most of the probiotics Bacterial cells, express native antibacterial genes, resulting in the production of, antimicrobial peptides, which have various applications in biotechnology and drug development. But the identification of antibacterial peptide, structural characterization of antimicrobial peptide and prediction on mode of action. Regardless of the significance of protein manufacturing, three individual factors are required for the production method: gene expression, stabilization and specific peptide purification. Our protocol describes a straightforward technique of detecting and characterizing particular extracellular peptides and enhancing the antimicrobial peptide expression we optimized using low molecular weight peptides. This protocol can be used to improve peptide detection and expression. The following are the benefits of this method, (DOI – https://doi.org/10.1016/j.ijbiomac.2019.10.196 [1]).The data briefly describe a simple method in detection identification, characterization of antimicrobial extracellular peptide, predicating the mode of action of peptide in targeting pathogens (In-silico method), brief method on profiling of antimicrobial peptide and its mode of action [1]. Further the protocol can be used to enhance the specific peptide expressions, detection of peptides. The advantages of this technique are presented below: • Characterization protocol of specific antimicrobial peptide • The folded antimicrobial peptide expression were less expressed or non-expressed peptides. • Besides being low cost, less time-consuming, easy to handle, universal and fast to execute, the suggested technique can be used for multiple proteins expressed in probiotics (Lactobacillus species) expression system. |
| first_indexed | 2024-12-23T10:56:04Z |
| format | Article |
| id | doaj.art-e31061f3d147400891af2b8e267dec35 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-23T10:56:04Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-e31061f3d147400891af2b8e267dec352022-12-21T17:49:47ZengElsevierData in Brief2352-34092020-08-0131105745An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular dockingRamachandran Chelliah0Kandasamy Saravanakumar1Eric Banan-Mwine Daliri2Joong-Hark Kim3Jung-Kun Lee4Hyeon-yeong Jo5Inamul Hasan Madar6Se-Hun Kim7Sudha Rani Ramakrishnan8Momna Rubab9Kaliyan Barathikannan10Fred Kwame Ofosu11Hwang Subin12Park Eun-ji13Fazle Elahi14Myeong-Hyeon Wang15Deog-Hwan Oh16Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea; Erom, Co., Ltd., Chuncheon, Gangwon-do 24427, KoreaDepartment of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea; Erom, Co., Ltd., Chuncheon, Gangwon-do 24427, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Biochemistry, School of Life Science, Bharathidasan, University, Thiruchirappalli, Tamilnadu, IndiaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu, South KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea; Corresponding author.Most of the probiotics Bacterial cells, express native antibacterial genes, resulting in the production of, antimicrobial peptides, which have various applications in biotechnology and drug development. But the identification of antibacterial peptide, structural characterization of antimicrobial peptide and prediction on mode of action. Regardless of the significance of protein manufacturing, three individual factors are required for the production method: gene expression, stabilization and specific peptide purification. Our protocol describes a straightforward technique of detecting and characterizing particular extracellular peptides and enhancing the antimicrobial peptide expression we optimized using low molecular weight peptides. This protocol can be used to improve peptide detection and expression. The following are the benefits of this method, (DOI – https://doi.org/10.1016/j.ijbiomac.2019.10.196 [1]).The data briefly describe a simple method in detection identification, characterization of antimicrobial extracellular peptide, predicating the mode of action of peptide in targeting pathogens (In-silico method), brief method on profiling of antimicrobial peptide and its mode of action [1]. Further the protocol can be used to enhance the specific peptide expressions, detection of peptides. The advantages of this technique are presented below: • Characterization protocol of specific antimicrobial peptide • The folded antimicrobial peptide expression were less expressed or non-expressed peptides. • Besides being low cost, less time-consuming, easy to handle, universal and fast to execute, the suggested technique can be used for multiple proteins expressed in probiotics (Lactobacillus species) expression system.http://www.sciencedirect.com/science/article/pii/S2352340920306399antimicrobial peptidemode of action, structural characterizationgene expressionprimers designed based on protein motifidentification of antimicrobial peptide or functional peptide |
| spellingShingle | Ramachandran Chelliah Kandasamy Saravanakumar Eric Banan-Mwine Daliri Joong-Hark Kim Jung-Kun Lee Hyeon-yeong Jo Inamul Hasan Madar Se-Hun Kim Sudha Rani Ramakrishnan Momna Rubab Kaliyan Barathikannan Fred Kwame Ofosu Hwang Subin Park Eun-ji Fazle Elahi Myeong-Hyeon Wang Deog-Hwan Oh An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking Data in Brief antimicrobial peptide mode of action, structural characterization gene expression primers designed based on protein motif identification of antimicrobial peptide or functional peptide |
| title | An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking |
| title_full | An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking |
| title_fullStr | An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking |
| title_full_unstemmed | An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking |
| title_short | An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking |
| title_sort | effective datasets describing antimicrobial peptide produced from pediococcus acidilactici purification and mode of action determined by molecular docking |
| topic | antimicrobial peptide mode of action, structural characterization gene expression primers designed based on protein motif identification of antimicrobial peptide or functional peptide |
| url | http://www.sciencedirect.com/science/article/pii/S2352340920306399 |
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