Misidentification of prostamides as prostaglandins
Prostaglandins and endogenous cannabinoid metabolites share the same lipid backbone with differing polar head groups at exactly the position through which a large molecule is attached to provide antigenicity and thus raise antisera. Hence, we hypothesized that antisera raised against prostaglandins...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2005-07-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520329862 |
| _version_ | 1818646816747421696 |
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| author | Michelle Glass Jiwon Hong Timothy A. Sato Murray D. Mitchell |
| author_facet | Michelle Glass Jiwon Hong Timothy A. Sato Murray D. Mitchell |
| author_sort | Michelle Glass |
| collection | DOAJ |
| description | Prostaglandins and endogenous cannabinoid metabolites share the same lipid backbone with differing polar head groups at exactly the position through which a large molecule is attached to provide antigenicity and thus raise antisera. Hence, we hypothesized that antisera raised against prostaglandins linked to a large molecule such as BSA at the carboxyl functional group would also recognize endogenous cannabinoid metabolites and lead to highly misleading interpretations of data. We found major cross-reactivity of commercial antisera raised to prostaglandins with endocannabinoid metabolites. Furthermore, in a well-characterized cell line (WISH) or primary amnion tissue explants, endocannabinoid treatment led to increased production of endocannabinoid metabolites as opposed to primary prostaglandins. This was apparent only after separation of products by thin-layer chromatography, because they measured as prostaglandins by radioimmunoassay.These findings have major implications for our interpretation of data in situations in which these prostaglandin-like molecules are formed, and they stress the need for chromatographic or spectrometric confirmation of prostaglandin production detected by antibody-based methods. |
| first_indexed | 2024-12-17T00:52:39Z |
| format | Article |
| id | doaj.art-e31127ffc8dd4526943cc0b4ac95f094 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T00:52:39Z |
| publishDate | 2005-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-e31127ffc8dd4526943cc0b4ac95f0942022-12-21T22:09:44ZengElsevierJournal of Lipid Research0022-22752005-07-0146713641368Misidentification of prostamides as prostaglandinsMichelle Glass0Jiwon Hong1Timothy A. Sato2Murray D. Mitchell3To whom correspondence should be addressed.; Department of Pharmacology, Auckland, New ZealandDepartment of Pharmacology, Auckland, New ZealandLiggins Institute, Auckland, New ZealandDepartment of Pharmacology, Auckland, New Zealand; Liggins Institute, Auckland, New Zealand; University of Auckland, and National Research Centre for Growth and Development, Auckland, New ZealandProstaglandins and endogenous cannabinoid metabolites share the same lipid backbone with differing polar head groups at exactly the position through which a large molecule is attached to provide antigenicity and thus raise antisera. Hence, we hypothesized that antisera raised against prostaglandins linked to a large molecule such as BSA at the carboxyl functional group would also recognize endogenous cannabinoid metabolites and lead to highly misleading interpretations of data. We found major cross-reactivity of commercial antisera raised to prostaglandins with endocannabinoid metabolites. Furthermore, in a well-characterized cell line (WISH) or primary amnion tissue explants, endocannabinoid treatment led to increased production of endocannabinoid metabolites as opposed to primary prostaglandins. This was apparent only after separation of products by thin-layer chromatography, because they measured as prostaglandins by radioimmunoassay.These findings have major implications for our interpretation of data in situations in which these prostaglandin-like molecules are formed, and they stress the need for chromatographic or spectrometric confirmation of prostaglandin production detected by antibody-based methods.http://www.sciencedirect.com/science/article/pii/S0022227520329862ethanolamidesprostamidesendocannabinoidsanandamide |
| spellingShingle | Michelle Glass Jiwon Hong Timothy A. Sato Murray D. Mitchell Misidentification of prostamides as prostaglandins Journal of Lipid Research ethanolamides prostamides endocannabinoids anandamide |
| title | Misidentification of prostamides as prostaglandins |
| title_full | Misidentification of prostamides as prostaglandins |
| title_fullStr | Misidentification of prostamides as prostaglandins |
| title_full_unstemmed | Misidentification of prostamides as prostaglandins |
| title_short | Misidentification of prostamides as prostaglandins |
| title_sort | misidentification of prostamides as prostaglandins |
| topic | ethanolamides prostamides endocannabinoids anandamide |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520329862 |
| work_keys_str_mv | AT michelleglass misidentificationofprostamidesasprostaglandins AT jiwonhong misidentificationofprostamidesasprostaglandins AT timothyasato misidentificationofprostamidesasprostaglandins AT murraydmitchell misidentificationofprostamidesasprostaglandins |